{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,24]],"date-time":"2026-10-24T01:40:07Z","timestamp":1792806007130,"version":"4.5.0"},"reference-count":112,"publisher":"Oxford University Press (OUP)","issue":"11","license":[{"start":{"date-parts":[[2023,9,7]],"date-time":"2023-09-07T00:00:00Z","timestamp":1694044800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100000065","name":"NINDS","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000065","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","award":["R01-NS078375"],"award-info":[{"award-number":["R01-NS078375"]}],"id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Neuroscience Research","award":["R01-AA027079"],"award-info":[{"award-number":["R01-AA027079"]}]},{"DOI":"10.13039\/100000894","name":"SMA Foundation","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000894","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004923","name":"AFM T\u00e9l\u00e9thon","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004923","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,11,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Neuromodulation by serotonin regulates the activity of neuronal networks responsible for a wide variety of essential behaviours. Serotonin (or 5-HT) typically activates metabotropic G protein-coupled receptors, which in turn initiate second messenger signalling cascades and induce short and long-lasting behavioural effects. Serotonin is intricately involved in the production of locomotor activity and gait control for different motor behaviours. Although dysfunction of serotonergic neurotransmission has been associated with mood disorders and spasticity after spinal cord injury, whether and to what extent such dysregulation is implicated in movement disorders has not been firmly established.<\/jats:p>\n                  <jats:p>Here, we investigated whether serotonergic neuromodulation is affected in spinal muscular atrophy (SMA), a neurodegenerative disease caused by ubiquitous deficiency of the SMN protein. The hallmarks of SMA are death of spinal motor neurons, muscle atrophy and impaired motor control, both in human patients and mouse models of disease. We used a severe mouse model of SMA, that closely recapitulates the severe symptoms exhibited by type I SMA patients, the most common and most severe form of the disease. Together, with mouse genetics, optogenetics, physiology, morphology and behavioural analysis, we report severe dysfunction of serotonergic neurotransmission in the spinal cord of SMA mice, both at early and late stages of the disease. This dysfunction is followed by reduction of 5-HT synapses on vulnerable motor neurons.<\/jats:p>\n                  <jats:p>We demonstrate that motor neurons innervating axial and trunk musculature are preferentially affected, suggesting a possible cause for the proximo-distal progression of disease, and raising the possibility that it may underlie scoliosis in SMA patients. We also demonstrate that the 5-HT dysfunction is caused by SMN deficiency in serotonergic neurons in the raphe nuclei of the brainstem. The behavioural significance of the dysfunction in serotonergic neuromodulation is underlined by inter-limb discoordination in SMA mice, which is ameliorated when selective restoration of SMN in 5-HT neurons is achieved by genetic means.<\/jats:p>\n                  <jats:p>Our study uncovers an unexpected dysfunction of serotonergic neuromodulation in SMA and indicates that, if normal function is to be restored under disease conditions, 5-HT neuromodulation should be a key target for therapeutic approaches.<\/jats:p>","DOI":"10.1093\/brain\/awad221","type":"journal-article","created":{"date-parts":[[2023,7,3]],"date-time":"2023-07-03T16:51:56Z","timestamp":1688403116000},"page":"4574-4593","source":"Crossref","is-referenced-by-count":14,"title":["Serotonergic dysfunction impairs locomotor coordination in spinal muscular atrophy"],"prefix":"10.1093","volume":"146","author":[{"given":"Nicolas","family":"Delestr\u00e9e","sequence":"first","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Neurology, Columbia University , New York, NY 10032 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9753-6124","authenticated-orcid":false,"given":"Evangelia","family":"Semizoglou","sequence":"additional","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Neurology, Columbia University , New York, NY 10032 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John G","family":"Pagiazitis","sequence":"additional","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Neurology, Columbia University , New York, NY 10032 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aleksandra","family":"Vukojicic","sequence":"additional","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Neurology, Columbia University , New York, NY 10032 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Estelle","family":"Drobac","sequence":"additional","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Pathology and Cell Biology, Columbia University , New York, NY 10032 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasilissa","family":"Paushkin","sequence":"additional","affiliation":[{"name":"Center for Motor Neuron Biology and Disease, Columbia University , New York, NY 10032 , USA"},{"name":"Department of Neurology, Columbia University , New York, NY 10032 , 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