{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,16]],"date-time":"2026-10-16T11:52:13Z","timestamp":1792151533255,"version":"4.4.0"},"reference-count":87,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,9,30]],"date-time":"2023-09-30T00:00:00Z","timestamp":1696032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"DOI":"10.13039\/100002243","name":"National Ataxia Foundation","doi-asserted-by":"publisher","award":["NIH K99 NS124994"],"award-info":[{"award-number":["NIH K99 NS124994"]}],"id":[{"id":"10.13039\/100002243","id-type":"DOI","asserted-by":"publisher"}]},{"name":"RNA Institute Summer Bioinformatics Fellowships"},{"name":"Albany Summer Research Program Fellowship","award":["NIH R21 NS114794"],"award-info":[{"award-number":["NIH R21 NS114794"]}]},{"name":"Albany Summer Research Program Fellowship","award":["NIH P50 NS04843"],"award-info":[{"award-number":["NIH P50 NS04843"]}]},{"name":"Albany Summer Research Program Fellowship","award":["R01 NS120485"],"award-info":[{"award-number":["R01 NS120485"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,2,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The spinocerebellar ataxias (SCAs) are a group of dominantly inherited neurodegenerative diseases, several of which are caused by CAG expansion mutations (SCAs 1, 2, 3, 6, 7 and 12) and more broadly belong to the large family of over 40 microsatellite expansion diseases. While dysregulation of alternative splicing is a well defined driver of disease pathogenesis across several microsatellite diseases, the contribution of alternative splicing in CAG expansion SCAs is poorly understood. Furthermore, despite extensive studies on differential gene expression, there remains a gap in our understanding of presymptomatic transcriptomic drivers of disease. We sought to address these knowledge gaps through a comprehensive study of 29 publicly available RNA-sequencing datasets.<\/jats:p>\n                  <jats:p>We identified that dysregulation of alternative splicing is widespread across CAG expansion mouse models of SCAs 1, 3 and 7. These changes were detected presymptomatically, persisted throughout disease progression, were repeat length-dependent, and were present in brain regions implicated in SCA pathogenesis including the cerebellum, pons and medulla. Across disease progression, changes in alternative splicing occurred in genes that function in pathways and processes known to be impaired in SCAs, such as ion channels, synaptic signalling, transcriptional regulation and the cytoskeleton. We validated several key alternative splicing events with known functional consequences, including Trpc3 exon 9 and Kcnma1 exon 23b, in the Atxn1154Q\/2Q mouse model. Finally, we demonstrated that alternative splicing dysregulation is responsive to therapeutic intervention in CAG expansion SCAs with Atxn1 targeting antisense oligonucleotide rescuing key splicing events.<\/jats:p>\n                  <jats:p>Taken together, these data demonstrate that widespread presymptomatic dysregulation of alternative splicing in CAG expansion SCAs may contribute to disease onset, early neuronal dysfunction and may represent novel biomarkers across this devastating group of neurodegenerative disorders.<\/jats:p>","DOI":"10.1093\/brain\/awad329","type":"journal-article","created":{"date-parts":[[2023,9,28]],"date-time":"2023-09-28T16:28:17Z","timestamp":1695918497000},"page":"486-504","source":"Crossref","is-referenced-by-count":19,"title":["Widespread alternative splicing dysregulation occurs presymptomatically in CAG expansion spinocerebellar ataxias"],"prefix":"10.1093","volume":"147","author":[{"given":"Hannah K","family":"Shorrock","sequence":"first","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Claudia D","family":"Lennon","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Asmer","family":"Aliyeva","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"},{"name":"Department of Biology, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Emily E","family":"Davey","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cristina C","family":"DeMeo","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caroline E","family":"Pritchard","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lori","family":"Planco","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose M","family":"Velez","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"},{"name":"Department of Biology, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Mascorro-Huamancaja","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Damian S","family":"Shin","sequence":"additional","affiliation":[{"name":"Department of Neuroscience and Experimental Therapeutics, Albany Medical College , Albany, NY 12208 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"John D","family":"Cleary","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5198-2724","authenticated-orcid":false,"given":"J Andrew","family":"Berglund","sequence":"additional","affiliation":[{"name":"The RNA Institute, University at Albany\u2014SUNY , Albany, NY 12222 , USA"},{"name":"Department of Biology, University at Albany\u2014SUNY , Albany, NY 12222 , USA"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,9,30]]},"reference":[{"key":"2024020201482224600_awad329-B1","doi-asserted-by":"crossref","first-page":"590","DOI":"10.1038\/s41582-018-0051-6","article-title":"Spinocerebellar ataxias: Prospects and challenges for therapy development","volume":"14","author":"Ashizawa","year":"2018","journal-title":"Nat Rev Neurol"},{"key":"2024020201482224600_awad329-B2","doi-asserted-by":"crossref","first-page":"24","DOI":"10.1038\/s41572-019-0074-3","article-title":"Spinocerebellar ataxia","volume":"5","author":"Klockgether","year":"2019","journal-title":"Nat Rev Dis Primers"},{"key":"2024020201482224600_awad329-B3","doi-asserted-by":"crossref","first-page":"613","DOI":"10.1038\/nrn.2017.92","article-title":"Polyglutamine spinocerebellar ataxias\u2014From genes to potential 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