{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,19]],"date-time":"2026-10-19T12:43:16Z","timestamp":1792413796674,"version":"4.4.0"},"reference-count":51,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,9,15]],"date-time":"2026-09-15T00:00:00Z","timestamp":1789430400000},"content-version":"am","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"CReATe Consortium","award":["U54 NS092091"],"award-info":[{"award-number":["U54 NS092091"]}]},{"DOI":"10.13039\/100000002","name":"NIH","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100000002","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100016650","name":"Rare Diseases Clinical Research Network","doi-asserted-by":"publisher","award":["RF1 NS105479"],"award-info":[{"award-number":["RF1 NS105479"]}],"id":[{"id":"10.13039\/100016650","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>In the effort to reduce the long diagnostic delay in amyotrophic lateral sclerosis, an often overlooked problem derives from confusion around the concept of diagnostic biomarkers and the type of data required to develop such tools. A useful diagnostic biomarker is one that can correctly differentiate individuals with, from those without, the disease of interest\u2014and importantly, at a point in time when the diagnosis is otherwise uncertain. This requires a test with a high positive predictive value (the proportion of positive test results that are true positives), a high negative predictive value (the proportion of negative test results that are true negatives) or ideally both. Critically, positive and negative predictive values depend on the context of use for the diagnostic, which determines the prevalence of disease in the population in which the diagnostic test will be used. Almost all diagnostic biomarker studies in amyotrophic lateral sclerosis get this wrong\u2014focusing on data and samples from patients for whom there is no diagnostic uncertainty.<\/jats:p>\n                  <jats:p>This update distinguishes disease state biomarkers from diagnostic biomarkers; highlights the data required to clinically validate a diagnostic biomarker; underscores the critical importance of understanding the clinical context in which the diagnostic biomarker will be used; and outlines potential strategies for developing diagnostic biomarkers that might be clinically useful.<\/jats:p>","DOI":"10.1093\/brain\/awag313","type":"journal-article","created":{"date-parts":[[2026,9,11]],"date-time":"2026-09-11T11:52:41Z","timestamp":1789127561000},"source":"Crossref","is-referenced-by-count":0,"title":["The diagnostic biomarker problem in amyotrophic lateral sclerosis"],"prefix":"10.1093","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-4241-5135","authenticated-orcid":false,"given":"Michael","family":"Benatar","sequence":"first","affiliation":[{"name":"Department of Neurology and the ALS Center, University of Miami Miller School of Medicine , Miami, FL 33136 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0005-9643-9855","authenticated-orcid":false,"given":"Joanne","family":"Wuu","sequence":"additional","affiliation":[{"name":"Department of Neurology and the ALS Center, University of Miami Miller School of Medicine , Miami, FL 33136 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-0267-3180","authenticated-orcid":false,"given":"Martin R","family":"Turner","sequence":"additional","affiliation":[{"name":"Nuffield Department of Clinical Neurosciences, Oxford University , Oxford OX3 9DU ,","place":["UK"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1618-3502","authenticated-orcid":false,"given":"Eric A","family":"Macklin","sequence":"additional","affiliation":[{"name":"MGH Biostatistics, Massachusetts General Hospital, Harvard Medical School , Boston, MA 02114 ,","place":["USA"]},{"name":"Department of Neurology, Harvard Medical School , Boston, MA 02114 ,","place":["USA"]},{"name":"Department of Medicine, Harvard Medical School , Boston, MA 02114 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,9,15]]},"reference":[{"key":"2026101908012141100_awag313-B1","doi-asserted-by":"crossref","first-page":"453","DOI":"10.3109\/21678421.2014.903974","article-title":"Diagnostic timelines and delays in diagnosing amyotrophic lateral sclerosis (ALS)","volume":"15","author":"Paganoni","year":"2014","journal-title":"Amyotroph Lateral Scler Frontotemporal 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