{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,5]],"date-time":"2025-11-05T06:40:56Z","timestamp":1762324856512,"version":"3.41.2"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T00:00:00Z","timestamp":1605484800000},"content-version":"vor","delay-in-days":320,"URL":"http:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"funder":[{"DOI":"10.13039\/501100003000","name":"Dutch Multiple Sclerosis Research Foundation","doi-asserted-by":"publisher","award":["15-490d MS"],"award-info":[{"award-number":["15-490d MS"]}],"id":[{"id":"10.13039\/501100003000","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,7,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Neuromyelitis optica spectrum disorders are a group of rare, but severe autoimmune diseases characterized by inflammation of the optic nerve(s) and\/or spinal cord. Although naive B cells are considered key players by escaping central tolerance checkpoints, it remains unclear how their composition and outgrowth differ in patients with neuromyelitis optica spectrum disorders. Under complete treatment-naive circumstances, we found that naive mature\/transitional B-cell ratios were reduced in the blood of 10 patients with aquaporin-4 immunoglobulin G-positive disease (neuromyelitis optica spectrum disorders) as compared to 11 both age- and gender-matched healthy controls, eight patients with myelin oligodendrocyte glycoprotein-immunoglobulin G-associated disorders and 10 patients with multiple sclerosis. This was the result of increased proportions of transitional B cells, which were the highest in patients with neuromyelitis optica spectrum disorders with relapses and strongly diminished in a separate group of nine patients with neuromyelitis optica spectrum disorders and myelin oligodendrocyte glycoprotein-immunoglobulin G-associated disorders who received corticosteroid treatment. These findings need to be confirmed in longitudinal studies. For purified naive mature B cells of seven patients with neuromyelitis optica spectrum disorders and myelin oligodendrocyte glycoprotein-immunoglobulin G-associated disorders with relapses, Toll-like receptor 9 ligand synergized with interferon-\u03b3 to enhance plasmablast formation during germinal centre-like cultures. This was not seen for 11 patients without relapses and nine healthy controls. In the neuromyelitis optica spectrum disorders group, in vitro plasmablast formation corresponded to total and anti-aquaporin-4 immunoglobulin G secretion, of which the latter was found only for relapsing cases. These data indicate that naive B-cell homoeostasis is different and selectively targeted by corticosteroids in patients with neuromyelitis optica spectrum disorders. This also supports further exploration of naive B cells for their use in Toll-like receptor 9-dependent in vitro platforms in order to predict the activity of neuromyelitis optica spectrum disorders.<\/jats:p>","DOI":"10.1093\/braincomms\/fcaa197","type":"journal-article","created":{"date-parts":[[2020,11,16]],"date-time":"2020-11-16T17:34:56Z","timestamp":1605548096000},"source":"Crossref","is-referenced-by-count":10,"title":["Naive B cells in neuromyelitis optica spectrum disorders: impact of steroid use and relapses"],"prefix":"10.1093","volume":"2","author":[{"given":"Malou","family":"Janssen","sequence":"first","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"},{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7990-5894","authenticated-orcid":false,"given":"Arlette L","family":"Bruijstens","sequence":"additional","affiliation":[{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jamie","family":"van Langelaar","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"YuYi","family":"Wong","sequence":"additional","affiliation":[{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Annet F","family":"Wierenga-Wolf","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marie-Jos\u00e9","family":"Melief","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2294-2774","authenticated-orcid":false,"given":"Liza","family":"Rijvers","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"E Dani\u00eblle","family":"van Pelt","sequence":"additional","affiliation":[{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9766-8661","authenticated-orcid":false,"given":"Joost","family":"Smolders","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The Netherlands"},{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Beatrijs H","family":"Wokke","sequence":"additional","affiliation":[{"name":"Department of Neurology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GD, The Netherlands"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marvin M","family":"van Luijn","sequence":"additional","affiliation":[{"name":"Department of Immunology, MS Center ErasMS, Erasmus MC, Rotterdam, 3015 GE, The 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