{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:48:09Z","timestamp":1753876089693,"version":"3.41.2"},"reference-count":40,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T00:00:00Z","timestamp":1745798400000},"content-version":"vor","delay-in-days":117,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Sociedade Portuguesa de Cefaleias"},{"DOI":"10.13039\/100004336","name":"Novartis","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100004336","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Unit for Multidisciplinary Research in Biomedicine","award":["UIDB\/00215\/2020","UIDP\/00215\/2020"],"award-info":[{"award-number":["UIDB\/00215\/2020","UIDP\/00215\/2020"]}]},{"name":"Laboratory for Integrative and Translational Research in Population Health","award":["LA\/P\/0064\/2020"],"award-info":[{"award-number":["LA\/P\/0064\/2020"]}]},{"DOI":"10.13039\/501100001871","name":"Funda\u00e7\u00e3o para a Ci\u00eancia e a Tecnologia","doi-asserted-by":"publisher","award":["POCI-01-0145-FEDER- 029486","PTDC\/MEC-NEU\/29486\/2017"],"award-info":[{"award-number":["POCI-01-0145-FEDER- 029486","PTDC\/MEC-NEU\/29486\/2017"]}],"id":[{"id":"10.13039\/501100001871","id-type":"DOI","asserted-by":"publisher"}]},{"name":"FCT fellowships","award":["SFRH\/BD\/136954\/2018","2020.05763.BD"],"award-info":[{"award-number":["SFRH\/BD\/136954\/2018","2020.05763.BD"]}]},{"DOI":"10.13039\/501100009091","name":"FCT","doi-asserted-by":"publisher","award":["DL 57\/2016"],"award-info":[{"award-number":["DL 57\/2016"]}],"id":[{"id":"10.13039\/501100009091","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,4]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Migraine is a complex neurological disorder, and the most common migraine categories are migraine with aura and without aura. The higher prevalence of migraine in related individuals compared to the general population indicates a potential genetic predisposition; however, gene expression, which is influenced by both genetic and environmental factors, can also be a major factor in the migraine susceptibility. Given the high number of Portuguese migraine patients whose diagnosis and treatment have not yet been well established, we decided to carry out a whole transcriptome analysis within a migraine Portuguese cohort. This study aims to identify potential biomarkers that could contribute to improved migraine therapy. We performed total RNA sequencing on whole blood samples from 15 migraine patients and 12 age-matched controls. Differential expression analysis and gene set enrichment analysis were performed in different migraine subgroups. Finally, we performed the protein-protein interaction networks of differentially expressed genes. Gene set enrichment analysis comparing migraine patients with controls highlighted upregulated pathways linked to metabolism, and downregulated immuno-inflammatory pathways. Moreover, the groups of female migraine patients and female migraine without aura patients emphasized significant upregulated pathways, including G protein-coupled receptors signalling pathways, when compared with female controls. Interestingly, we found two important differentially expressed genes related to parathyroid hormone: PTH1R and PTH2. PTH1R was upregulated in female migraine without aura versus female controls, while PTH2 was both upregulated between female migraine patients and female controls, as well as between female migraine without aura and controls. Here, we show, for the first time, the involvement of parathyroid hormone receptors and their associated gene expression patterns in female migraine patients. These molecules stand out as sturdy and promising biomarkers for innovative therapeutic in female migraine patients.<\/jats:p>","DOI":"10.1093\/braincomms\/fcaf142","type":"journal-article","created":{"date-parts":[[2025,4,28]],"date-time":"2025-04-28T02:47:07Z","timestamp":1745808427000},"source":"Crossref","is-referenced-by-count":0,"title":["Decoding migraine disorders: parathyroid hormone-related peptide receptors as key genetic drivers"],"prefix":"10.1093","volume":"7","author":[{"given":"Andreia","family":"Dias","sequence":"first","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , Porto 4200-135 ,","place":["Portugal"]},{"name":"Unidade Multidisciplinar de Investiga\u00e7\u00e3o Biom\u00e9dica (UMIB), Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto , Porto 4050-313 ,","place":["Portugal"]},{"name":"ITR\u2014Laboratory for Integrative and Translational Research in Population Health , Porto 4050-600 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Marta","family":"Ferreira","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , Porto 4200-135 ,","place":["Portugal"]},{"name":"IPATIMUP\u2014Instituto de Patologia e Imunologia Molecular da Universidade do Porto , Universidade do Porto, Porto 4200-135 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2343-2215","authenticated-orcid":false,"given":"Mariana","family":"Santos","sequence":"additional","affiliation":[{"name":"Unidade Multidisciplinar de Investiga\u00e7\u00e3o Biom\u00e9dica (UMIB), Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto , Porto 4050-313 ,","place":["Portugal"]},{"name":"ITR\u2014Laboratory for Integrative and Translational Research in Population Health , Porto 4050-600 ,","place":["Portugal"]},{"name":"IBMC\u2014Instituto de Biologia Molecular e Celular , Universidade do Porto, Porto 4200-135 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alda","family":"Sousa","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , Porto 4200-135 ,","place":["Portugal"]},{"name":"Unidade Multidisciplinar de Investiga\u00e7\u00e3o Biom\u00e9dica (UMIB), Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto , Porto 4050-313 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8340-2264","authenticated-orcid":false,"given":"Carla","family":"Oliveira","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , Porto 4200-135 ,","place":["Portugal"]},{"name":"IPATIMUP\u2014Instituto de Patologia e Imunologia Molecular da Universidade do Porto , Universidade do Porto, Porto 4200-135 ,","place":["Portugal"]},{"name":"FMUP\u2014Faculdade de Medicina da Universidade do Porto , Universidade do Porto, Porto 4200-319 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Miguel","family":"Alves-Ferreira","sequence":"additional","affiliation":[{"name":"Unidade Multidisciplinar de Investiga\u00e7\u00e3o Biom\u00e9dica (UMIB), Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto , Porto 4050-313 ,","place":["Portugal"]},{"name":"IBMC\u2014Instituto de Biologia Molecular e Celular , Universidade do Porto, Porto 4200-135 ,","place":["Portugal"]},{"name":"CGPP\u2014Centro de Gen\u00e9tica Preditiva e Preventiva , Universidade do Porto, Porto 4200-135 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Carolina","family":"Lemos","sequence":"additional","affiliation":[{"name":"i3S\u2014Instituto de Investiga\u00e7\u00e3o e Inova\u00e7\u00e3o em Sa\u00fade, Universidade do Porto , Porto 4200-135 ,","place":["Portugal"]},{"name":"Unidade Multidisciplinar de Investiga\u00e7\u00e3o Biom\u00e9dica (UMIB), Instituto de Ci\u00eancias Biom\u00e9dicas Abel Salazar (ICBAS), Universidade do Porto , Porto 4050-313 ,","place":["Portugal"]},{"name":"ITR\u2014Laboratory for Integrative and Translational Research in Population Health , Porto 4050-600 ,","place":["Portugal"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,4,28]]},"reference":[{"issue":"1","key":"2025042722470440400_fcaf142-B1","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1177\/0333102417738202","article-title":"The international classification of headache disorders, 3rd 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migraine","volume":"17","author":"Bron","year":"2021","journal-title":"Neuropsychiatr Dis Treat"},{"key":"2025042722470440400_fcaf142-B5","first-page":"301","article-title":"The impact of lifestyle factors on migraine frequency and severity in neuro-logy","volume":"12","author":"Iyun","year":"2024","journal-title":"J Biosci Med (Irvine)"},{"issue":"3","key":"2025042722470440400_fcaf142-B6","doi-asserted-by":"crossref","first-page":"404","DOI":"10.1111\/j.1526-4610.2008.01177.x","article-title":"Familial clustering of migraine: Further evidence from a Portuguese study","volume":"49","author":"Lemos","year":"2009","journal-title":"Headache"},{"issue":"1","key":"2025042722470440400_fcaf142-B7","doi-asserted-by":"crossref","first-page":"8294","DOI":"10.1038\/s41598-021-87503-5","article-title":"Changes in the gene expression profile during spontaneous migraine attacks","volume":"11","author":"Kogelman","year":"2021","journal-title":"Sci 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