{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T19:18:31Z","timestamp":1787167111719,"version":"build-2736575974"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,6,10]],"date-time":"2025-06-10T00:00:00Z","timestamp":1749513600000},"content-version":"vor","delay-in-days":160,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"German Research Council","award":["SI1886\/1-2"],"award-info":[{"award-number":["SI1886\/1-2"]}]},{"name":"German Research Council","award":["SI1886\/3-1"],"award-info":[{"award-number":["SI1886\/3-1"]}]},{"DOI":"10.13039\/501100017268","name":"Berlin Institute of Health","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100017268","id-type":"DOI","asserted-by":"publisher"}]},{"name":"clinician scientist program"},{"name":"translational PhD program"},{"name":"MedMS program of the Hertie Foundation"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Multiple sclerosis (MS) is a chronic inflammatory CNS disease with heterogeneous manifestation. Prognostic markers for early classification of MS are currently under investigation. Higher diagnostic resolution of cerebrospinal fluid (CSF) has the potential to contribute significantly to patient stratification, which should be especially important for a subgroup of patients with high risk to convert to a progressive disease course. This study aimed to determine whether spectral flow cytometry of CSF cells could identify pathogenic CD4+ T cell subset in MS. Using a two-step approach, we designed a marker panel informed by publicly available transcriptomic datasets from early human MS and our own single-cell RNA sequencing (scRNA-seq) in acute and chronic experimental autoimmune encephalomyelitis (EAE), a murine MS model. Notably, chronic (\u2018phase\u2019) markers such as Il7r and Ramp3 (associated with memory T cells), Itgb1 (integrin beta-1) and anti-apoptotic genes like Dnaja1, Hsph1 and Jun\/AP-1 were enriched in EAE. These markers reflect pro-survival signalling and tissue-residency characteristics, including CXCR6, CD69 and Bhlhe40, which suggest an adaptation of CD4+ T cells towards persistent neuroinflammatory responses in chronic EAE. This phase-specific marker profile highlights CD4+ T cells as both indicators and contributors to disease progression in EAE. Translating these findings to MS datasets, we found an enrichment of phase-specific markers in CSF cells. Spectral flow cytometry in an independent MS cohort revealed distinct memory and effector T cell subsets, indicating unique CSF signatures in MS. This study underscores the heterogeneity and dynamic changes of CD4+ T cells detectable by spectral flow cytometry, enhancing diagnostic resolution of CSF cells and informing more precise therapeutic strategies for MS.<\/jats:p>","DOI":"10.1093\/braincomms\/fcaf231","type":"journal-article","created":{"date-parts":[[2025,6,8]],"date-time":"2025-06-08T07:29:45Z","timestamp":1749367785000},"source":"Crossref","is-referenced-by-count":4,"title":["Mapping CD4+ T cell diversity in CSF to identify endophenotypes of multiple sclerosis"],"prefix":"10.1093","volume":"7","author":[{"given":"Tadhg","family":"Crowley","sequence":"first","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jessy","family":"Chen","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]},{"name":"Department of Neurology and Experimental Neurology, Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin , Berlin 13353 ,","place":["Germany"]},{"name":"Berlin Institute of Health (BIH), Charit\u00e9 Universit\u00e4tsmedizin Berlin , Berlin 10178 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Kamil S","family":"Rosiewicz","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 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Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Charlotte","family":"Biese","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Cornelius","family":"Fischer","sequence":"additional","affiliation":[{"name":"Berlin Institute of Health (BIH), Charit\u00e9 Universit\u00e4tsmedizin Berlin , Berlin 10178 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Janis","family":"Kerkering","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marlen","family":"Alisch","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Friedemann","family":"Paul","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research Center (ECRC), Charit\u00e9\u2014Universit\u00e4tsmedizin Berlin and Max Delbr\u00fcck Center or Molecular Medicine in the Helmholtz Association , Berlin 13125 ,","place":["Germany"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1532-2868","authenticated-orcid":false,"given":"Volker","family":"Siffrin","sequence":"additional","affiliation":[{"name":"Experimental and Clinical Research 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