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Our laboratory has reported that wild-type BRCA1 transcriptionally regulates \u03b2-hCG in triple negative breast cancers (TNBCs). These factors culminated into analysis of BRCA1 status in GTD, which would emanate into elucidation of BRCA1- \u03b2-hCG relationship and unraveling etio-pathology of GTD. BRCA1 level in GTD is down-regulated due to the over-expression of DNMT3b and subsequent promoter hypermethylation, when compared to the normal placentae accompanied with its shift in localization. There is an inverse correlation of serum \u03b2-hCG levels with BRCA1 mRNA expression. The effects of methotrexate (MTX), which is the first-line chemotherapeutic used for GTD treatment, when analyzed in comparison with plumbagin (PB) revealed that PB alone is efficient than MTX alone or MTX-PB in combination, in showing selective cytotoxicity against GTD. Interestingly, PB increases BRCA1 levels post-treatment, altering DNMT3b levels and resultant BRCA1 promoter methylation. Also, cohort study analyzed the incidence of GTD at Sree Avittom Thirunal (SAT) Hospital, Thiruvananthapuram, which points out that 11.5% of gestational trophoblastic neoplasia (GTN) cases were referred to Regional Cancer Centre, Thiruvananthapuram, for examination of breast lumps. This has lend clues to supervene the risk of GTD patients towards BRCA1-associated diseases and unveil novel therapeutic for GTD, a plant-derived naphthoquinone, PB, already reported as selectively cytotoxic against BRCA1 defective tumors.<\/jats:p>","DOI":"10.1093\/carcin\/bgz117","type":"journal-article","created":{"date-parts":[[2019,6,13]],"date-time":"2019-06-13T19:12:32Z","timestamp":1560453152000},"page":"611-624","source":"Crossref","is-referenced-by-count":7,"title":["BRCA1 promoter hypermethylation in human placenta: a hidden link with \u03b2-hCG expression"],"prefix":"10.1093","volume":"41","author":[{"given":"Revathy","family":"Nadhan","sequence":"first","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jayashree Vijaya","family":"Vaman","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, SAT Hospital, Government Medical College, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Satheesh Kumar","family":"Sengodan","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"},{"name":"Mouse Cancer Genetics Program, Center for Cancer Research, National Cancer Institute, Frederick, USA"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreelatha Krishnakumar","family":"Hemalatha","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"},{"name":"Department of Microbiology, Government Medical College, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nirmala","family":"Chellappan","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, SAT Hospital, Government Medical College, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santha","family":"Sadasivan","sequence":"additional","affiliation":[{"name":"Department of Pathology, Government Medical College, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Aysha","family":"Pasuthottiyil Varkey","sequence":"additional","affiliation":[{"name":"Department of Obstetrics and Gynecology, PRS Hospital, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreelekha","family":"Yesodharan","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Krishnapriya","family":"Raji Sathyanpillai","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amritha Krishna","family":"Bhuvaneswari Venugopal","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sreevidya","family":"Prameelakumari Sreenivasan","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Arathi","family":"Rajan","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Neetha Rajan","family":"Latha","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for 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Usha","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Thapasimuthu","family":"Vijayamma Anilkumar","sequence":"additional","affiliation":[{"name":"Department of Experimental Pathology, Biomedical Technology Wing, Sree Chitra Tirunal Institute for Medical Sciences and Technology, Thiruvananthapuram, Kerala, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Priya","family":"Srinivas","sequence":"additional","affiliation":[{"name":"Cancer Research Program 6, Rajiv Gandhi Centre for Biotechnology, Thiruvananthapuram, Kerala, 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