{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,25]],"date-time":"2026-03-25T22:14:45Z","timestamp":1774476885311,"version":"3.50.1"},"reference-count":34,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T00:00:00Z","timestamp":1696377600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001659","name":"Deutsche Forschungsgemeinschaft","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100001659","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,12,19]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Dermatitis herpetiformis (DH) is a rare gluten-induced skin disorder characterized predominantly by IgA autoantibodies against endomysium, tissue transglutaminase (TG2\/tTG), epidermal transglutaminase (TG3\/eTG) and deamidated gliadin. To date, circulating autoantibody reactivity has not been systematically described.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>Characterization of serum reactivities in DH.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This multicentre international study analysed sera from 242 patients with DH taken at the time of initial diagnosis. DH-specific IgA and IgG serum autoantibodies were analysed by indirect immunofluorescence (IF) on monkey oesophagus, and by enzyme-linked immunosorbent assay (ELISA) based on recombinant TG2\/tTG, TG3\/eTG and deamidated gliadin (GAF3X).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>IgA indirect IF microscopy on monkey oesophagus revealed the highest reactivity (84.3%; specificity 100%) followed by IgA TG2\/tTG ELISA (78.5%, specificity 99.0%), IgA TG3\/eTG ELISA (72.7%, specificity 95.0%) and IgA GAF3X ELISA (69.0%, specificity 98.5%).<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Serum IgA and IgG autoantibodies against endomysium, TG2\/tTG, TG3\/eTG and deamidated gliadin are highly prevalent in DH. Indirect IF microscopy on monkey oesophagus (IgA) provides the highest diagnostic accuracy that can be further enhanced by 4.5% when combined with IgA TG2\/tTG ELISA.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ced\/llad319","type":"journal-article","created":{"date-parts":[[2023,10,4]],"date-time":"2023-10-04T21:11:54Z","timestamp":1696453914000},"page":"53-57","source":"Crossref","is-referenced-by-count":2,"title":["Serum reactivity in dermatitis herpetiformis: an\u00a0international multicentre study"],"prefix":"10.1093","volume":"49","author":[{"given":"Katharina","family":"Boch","sequence":"first","affiliation":[{"name":"Department of Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Florian","family":"Heck","sequence":"additional","affiliation":[{"name":"L\u00fcbeck Institute of Experimental Dermatology"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph 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