{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,31]],"date-time":"2026-03-31T10:45:41Z","timestamp":1774953941592,"version":"3.50.1"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T00:00:00Z","timestamp":1722038400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,12,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:sec>\n                  <jats:title>Background<\/jats:title>\n                  <jats:p>Indirect immunofluorescence (IIF) plays a crucial role in the diagnosis of pemphigus and bullous pemphigoid (BP) by detecting the presence of circulating autoantibodies in the serum of patients. The standard serum transportation method requires delivery to laboratories at 2\u20138 \u00b0C within a day and storage at \u201320 to \u201380 \u00b0C. However, this protocol poses logistical challenges.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Objectives<\/jats:title>\n                  <jats:p>To carry out a study assessing how temperature variations affect the accuracy of IIF tests.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Methods<\/jats:title>\n                  <jats:p>This case\u2013control study analysed 203 serum specimens: 102 from patients with pemphigus and 101 from patients with BP. Specimens were stored at \u201380 \u00b0C (control), 24 \u00b0C and 40 \u00b0C for 7\u2005days before analysis to investigate variations in IIF titres between test and control conditions.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Results<\/jats:title>\n                  <jats:p>In pemphigus serum, 95% of specimens at 24 \u00b0C and 76% at 40 \u00b0C showed no titre difference compared with controls. Similarly, 89% of BP serum specimens at 24 \u00b0C and 82% at 40 \u00b0C matched the control titres. While 57 specimens across both groups experienced reduced titres, the decrease was primarily marginal (a 1-step reduction in 54 cases, 2-step in 3), with no transition from positive to negative results.<\/jats:p>\n               <\/jats:sec>\n               <jats:sec>\n                  <jats:title>Conclusions<\/jats:title>\n                  <jats:p>Storing serum at 24\u201340 \u00b0C for up to 7\u2005days before testing only slightly influences IIF outcomes for pemphigus and BP. These findings could prompt a significant revision in the existing strict transport guidelines, ensuring efficient use of resources without sacrificing the accuracy of diagnostic tests.<\/jats:p>\n               <\/jats:sec>","DOI":"10.1093\/ced\/llae289","type":"journal-article","created":{"date-parts":[[2024,7,27]],"date-time":"2024-07-27T18:20:29Z","timestamp":1722104429000},"page":"77-81","source":"Crossref","is-referenced-by-count":1,"title":["Revisiting serum storage protocols: assessing the impact of temperature variations on indirect immunofluorescence testing for pemphigus and bullous pemphigoid"],"prefix":"10.1093","volume":"50","author":[{"given":"Sumruay","family":"Pinkaew","sequence":"first","affiliation":[{"name":"Department of Dermatology, Faculty of Medicine Siriraj Hospital, Mahidol University , Bangkok 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