{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,9]],"date-time":"2026-10-09T03:14:12Z","timestamp":1791515652122,"version":"4.3.4"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2025,9,22]],"date-time":"2025-09-22T00:00:00Z","timestamp":1758499200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100003593","name":"Conselho Nacional de Desenvolvimento Cient\u00edfico e Tecnol\u00f3gico","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100003593","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100002322","name":"Coordena\u00e7\u00e3o de Aperfei\u00e7oamento de Pessoal de N\u00edvel Superior","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100002322","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,24]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:sec>\n                    <jats:title>Background<\/jats:title>\n                    <jats:p>Epidermodysplasia verruciformis (EV) is a rare genetic condition characterized by viral warts and an increased risk of squamous cell carcinoma (SCC). It is particularly associated with specific human papillomavirus (HPV) types, notably \u03b2-HPV variants 5 and 8. Toll-like receptors (TLRs) are crucial for immune response regulation and may contribute to cancer development in EV.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Objectives<\/jats:title>\n                    <jats:p>To assess the expression levels of TLR3, -4, -5 and -9 in SCC samples from patients with EV vs. those without EV to understand their potential roles in EV-related carcinogenesis.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Methods<\/jats:title>\n                    <jats:p>We examined tumour samples from 18 patients with EV alongside samples from 90 participants without EV. Techniques included tissue microarray analysis and conventional histological evaluation, with immunohistochemistry performed to detect TLR expression. Controls were included to validate the specificity of the results, and statistical analyses employed the Kruskal\u2013Wallis test with Dunn\u2013Bonferroni post hoc comparisons.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Results<\/jats:title>\n                    <jats:p>TLR3, -4, -5 and -9 were detected in both patient groups, with distinct expression patterns. TLR3 levels were higher in the healthy skin of patients with EV, while TLR9 was more prominent in tumours. In patients without EV, TLR3 and TLR4 expression varied with tumour stages. Invasive SCC showed increased TLR5 and TLR9 levels. EV samples showed significantly higher TLR4 and TLR9 expression than non-EV samples.<\/jats:p>\n                  <\/jats:sec>\n                  <jats:sec>\n                    <jats:title>Conclusions<\/jats:title>\n                    <jats:p>The differing TLR expression patterns indicate diverse immune mechanisms involved in EV-related carcinogenesis. This suggests that TLRs could be potential therapeutic targets, meriting further investigation into their roles in tumour growth regulation.<\/jats:p>\n                  <\/jats:sec>","DOI":"10.1093\/ced\/llaf429","type":"journal-article","created":{"date-parts":[[2025,9,19]],"date-time":"2025-09-19T11:59:45Z","timestamp":1758283185000},"page":"393-400","source":"Crossref","is-referenced-by-count":1,"title":["Toll-like receptor patterns in epidermodysplasia verruciformis-associated squamous cell carcinomas"],"prefix":"10.1093","volume":"51","author":[{"given":"Cibele C A","family":"Pereira","sequence":"first","affiliation":[{"name":"Department of Dermatology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"suffix":"Jr","given":"Luis Alberto R","family":"Fr\u00f3es\u00a0","sequence":"additional","affiliation":[{"name":"Department of Pathology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lana Luiza C","family":"Silva","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Maira G","family":"Saldanha","sequence":"additional","affiliation":[{"name":"Department of Gynecology, Federal University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Naiura\u00a0V","family":"Pereira","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Walmar R P","family":"Oliveira","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-6380-7192","authenticated-orcid":false,"given":"Mirian N","family":"Sotto","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]},{"name":"Department of Pathology, University of S\u00e3o Paulo Medical School , S\u00e3o Paulo, SP ,","place":["Brazil"]},{"name":"Tropical Medicine Institute, University of S\u00e3o Paulo , S\u00e3o Paulo, SP ,","place":["Brazil"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,9,22]]},"reference":[{"key":"2026022406172965000_llaf429-B1","first-page":"169","article-title":"Epidermodysplasia verruciformis: an autosomal recessive disease characterized by viral warts and skin cancer \u2013 a model for viral 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