{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,18]],"date-time":"2026-04-18T14:47:02Z","timestamp":1776523622801,"version":"3.51.2"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2021,11,29]],"date-time":"2021-11-29T00:00:00Z","timestamp":1638144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81870706"],"award-info":[{"award-number":["81870706"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32090052"],"award-info":[{"award-number":["32090052"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Guangdong Provincial Key Laboratory of Regional Immunity and Diseases","award":["2019B030301009"],"award-info":[{"award-number":["2019B030301009"]}]},{"name":"Shenzhen science, technology, and innovation committee","award":["KQTD20170331145453160"],"award-info":[{"award-number":["KQTD20170331145453160"]}]},{"name":"Shenzhen science, technology, and innovation committee","award":["KQJSCX20180328095619081"],"award-info":[{"award-number":["KQJSCX20180328095619081"]}]},{"name":"Shenzhen Longgang District Medical & Health Scientific Technology Research Programme","award":["LGKCYLWS2020002"],"award-info":[{"award-number":["LGKCYLWS2020002"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,1,28]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Female genital tract chronic inflammation is common in clinics; the pathogenesis is not fully understood yet. House dust mite (HDM) involves the pathogenesis of many chronic diseases in human. This study aims to identify HDM-specific allergic response in the cervix of patients with cervical inflammation. Patients (n = 80) with chronic cervicitis (CC) and non-CC control (NC) subjects (n = 80) were recruited into this study. Vaginal lavage fluids (VLF) were collected from CC patients and NC subjects. Cellular components and fluid part of VLF were separated by centrifugation, and analyzed by flow cytometry and enzyme-linked immunosorbent assay. We found that a portion (52 out of 80) of CC patients responded to HDM, manifesting positive skin prick test, and HDM-specific IgE and IgG was detected in the VLF (designated CCp patients). VLF of CCp patients showed a Th2-dominant profile. HDM-specific Th2 cells were detected in VLF in CCp patients. Exposure to HDM in the culture induced proinflammatory cytokine release from CCp VLF CD4+ T cells. Exposure to CCp VLF CD4+ T cell-conditioned medium induced de novo Th2 response. Direct exposure to HDM induced allergic response in the cervix of CCp patients. In summary, a portion of CC patients respond to HDM challenge in the cervix. Exposure to HDM induces an allergy-like response in the cervix of CCp patients.<\/jats:p>","DOI":"10.1093\/cei\/uxab026","type":"journal-article","created":{"date-parts":[[2022,1,12]],"date-time":"2022-01-12T18:52:25Z","timestamp":1642013545000},"page":"44-52","source":"Crossref","is-referenced-by-count":4,"title":["Characterization of allergic inflammation in chronic uterine cervicitis"],"prefix":"10.1093","volume":"207","author":[{"given":"Fei","family":"Ma","sequence":"first","affiliation":[{"name":"Department of Chinese Traditional Medicine, Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical University, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jun","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Medical Record Management, Shenzhen Maternal and Child HealthCare Hospital, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xiaodan","family":"Lv","sequence":"additional","affiliation":[{"name":"Department of Pediatrics, Longgang Maternal and Child HealthCare Hospital, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hua-Zhen","family":"Liu","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Shenzhen, China"},{"name":"Institute of Allergy and Immunology, Shenzhen University School of Medicine, State Key Laboratory of Respiratory Disease Allergy Division at Shenzhen University, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6806-9464","authenticated-orcid":false,"given":"Ping-Chang","family":"Yang","sequence":"additional","affiliation":[{"name":"Guangdong Provincial Key Laboratory of Regional Immunity and Diseases, Shenzhen, China"},{"name":"Institute of Allergy and Immunology, Shenzhen University School of Medicine, State Key Laboratory of Respiratory Disease Allergy Division at Shenzhen University, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yan","family":"Ning","sequence":"additional","affiliation":[{"name":"Department of Chinese Traditional Medicine, Affiliated Shenzhen Maternity & Child Healthcare Hospital, Southern Medical University, Shenzhen, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,11,29]]},"reference":[{"key":"2022040410021957200_CIT0001","doi-asserted-by":"crossref","first-page":"1","DOI":"10.1056\/NEJM198407053110101","article-title":"Mucopurulent cervicitis\u2013the ignored counterpart in women of urethritis in men.","volume":"311","author":"Brunham","year":"1984","journal-title":"N Engl J Med"},{"key":"2022040410021957200_CIT0002","doi-asserted-by":"crossref","first-page":"831","DOI":"10.1016\/0046-8177(90)90052-7","article-title":"Histopathology of endocervical infection caused by Chlamydia trachomatis, herpes simplex virus, Trichomonas vaginalis, and Neisseria gonorrhoeae.","volume":"21","author":"Kiviat","year":"1990","journal-title":"Hum Pathol"},{"key":"2022040410021957200_CIT0003","doi-asserted-by":"crossref","first-page":"598","DOI":"10.1097\/OLQ.0b013e3181b01948","article-title":"Mycoplasma genitalium as a contributor to the multiple etiologies of cervicitis in women attending sexually transmitted disease clinics.","volume":"36","author":"Gaydos","year":"2009","journal-title":"Sex Transm Dis"},{"key":"2022040410021957200_CIT0004","doi-asserted-by":"crossref","first-page":"475","DOI":"10.1097\/OLQ.0000000000000318","article-title":"Relationship of specific bacteria in the cervical and vaginal microbiotas with cervicitis.","volume":"42","author":"Gorgos","year":"2015","journal-title":"Sex Transm Dis"},{"key":"2022040410021957200_CIT0005","doi-asserted-by":"crossref","first-page":"359","DOI":"10.1016\/j.jpag.2011.06.006","article-title":"Cervicitis in adolescents: do clinicians understand diagnosis and treatment?","volume":"24","author":"Woods","year":"2011","journal-title":"J Pediatr Adolesc Gynecol"},{"key":"2022040410021957200_CIT0006","doi-asserted-by":"crossref","first-page":"83","DOI":"10.1155\/S1064744900000065","article-title":"Association of lower genital tract inflammation with objective evidence of endometritis.","volume":"8","author":"Peipert","year":"2000","journal-title":"Infect Dis Obstet Gynecol"},{"key":"2022040410021957200_CIT0007","doi-asserted-by":"crossref","first-page":"198","DOI":"10.1097\/00007435-199207000-00003","article-title":"Mucopurulent cervicitis as a predictor of chlamydial infection and adverse pregnancy outcome. The Investigators of the Johns Hopkins Study of Cervicitis and Adverse Pregnancy Outcome.","volume":"19","author":"Nugent","year":"1992","journal-title":"Sex Transm Dis"},{"key":"2022040410021957200_CIT0008","doi-asserted-by":"crossref","first-page":"100057","DOI":"10.1016\/j.waojou.2019.100057","article-title":"Asthma and allergic rhinitis risk depends on house dust mite specific IgE levels in PARIS birth cohort children.","volume":"12","author":"Gabet","year":"2019","journal-title":"World Allergy Organ J"},{"key":"2022040410021957200_CIT0009","doi-asserted-by":"crossref","first-page":"1435","DOI":"10.1038\/s41590-019-0493-z","article-title":"House dust mites activate nociceptor-mast cell clusters to drive type 2 skin inflammation.","volume":"20","author":"Serhan","year":"2019","journal-title":"Nat Immunol"},{"key":"2022040410021957200_CIT0010","doi-asserted-by":"crossref","first-page":"8807","DOI":"10.7150\/thno.47134","article-title":"House dust mite specific antibodies induce neutrophilic inflammation in the heart.","volume":"10","author":"Chen","year":"2020","journal-title":"Theranostics"},{"key":"2022040410021957200_CIT0011","doi-asserted-by":"crossref","first-page":"1902375","DOI":"10.1183\/13993003.02375-2019","article-title":"Cross-reactive antibodies against dust mite-derived enolase induce neutrophilic airway inflammation.","volume":"57","author":"Lin","year":"2021","journal-title":"Eur Respir J"},{"key":"2022040410021957200_CIT0012","doi-asserted-by":"crossref","first-page":"757","DOI":"10.1136\/gutjnl-2015-310523","article-title":"Presence of commensal house dust mite allergen in human gastrointestinal tract: a potential contributor to intestinal barrier dysfunction.","volume":"65","author":"Tulic","year":"2016","journal-title":"Gut"},{"key":"2022040410021957200_CIT0013","doi-asserted-by":"crossref","first-page":"633","DOI":"10.1002\/JLB.3A0620-128R","article-title":"Enolase-specific cross antibodies induce neutrophilic inflammation in the intestine.","volume":"109","author":"Lin","year":"2021","journal-title":"J Leukoc Biol"},{"key":"2022040410021957200_CIT0014","doi-asserted-by":"crossref","first-page":"661","DOI":"10.1016\/j.eimc.2018.12.004","article-title":"Cervicitis: etiology, diagnosis and treatment.","volume":"37","author":"Ortiz-de la Tabla","year":"2019","journal-title":"Enferm Infecc Microbiol Clin (Engl Ed)"},{"key":"2022040410021957200_CIT0015","doi-asserted-by":"crossref","first-page":"985","DOI":"10.1016\/S1074-7613(01)00243-6","article-title":"IL-25 induces IL-4, IL-5, and IL-13 and Th2-associated pathologies in vivo.","volume":"15","author":"Fort","year":"2001","journal-title":"Immunity"},{"key":"2022040410021957200_CIT0016","doi-asserted-by":"crossref","first-page":"14","DOI":"10.1016\/j.cyto.2015.05.010","article-title":"T helper 2 (Th2) cell differentiation, type 2 innate lymphoid cell (ILC2) development and regulation of interleukin-4 (IL-4) and IL-13 production.","volume":"75","author":"Zhu","year":"2015","journal-title":"Cytokine"},{"key":"2022040410021957200_CIT0017","doi-asserted-by":"crossref","first-page":"975","DOI":"10.1016\/j.immuni.2019.03.018","article-title":"The cytokines of asthma.","volume":"50","author":"Lambrecht","year":"2019","journal-title":"Immunity"},{"key":"2022040410021957200_CIT0018","doi-asserted-by":"crossref","first-page":"e39905","DOI":"10.7554\/eLife.39905","article-title":"IgE-mediated mast cell activation promotes inflammation and cartilage destruction in osteoarthritis.","volume":"8","author":"Wang","year":"2019","journal-title":"Elife"},{"key":"2022040410021957200_CIT0019","doi-asserted-by":"crossref","first-page":"1441","DOI":"10.1172\/JCI124606","article-title":"Epithelial cell-derived cytokines: more than just signaling the alarm.","volume":"129","author":"Roan","year":"2019","journal-title":"J Clin Invest"},{"key":"2022040410021957200_CIT0020","doi-asserted-by":"crossref","first-page":"651","DOI":"10.3390\/medicina55100651","article-title":"Oral food challenge.","volume":"55","author":"Calvani","year":"2019","journal-title":"Medicina (Kaunas)"}],"container-title":["Clinical and Experimental Immunology"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/cei\/advance-article-pdf\/doi\/10.1093\/cei\/uxab026\/42295043\/uxab026.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/207\/1\/44\/43249297\/uxab026.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/cei\/article-pdf\/207\/1\/44\/43249297\/uxab026.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2022,4,4]],"date-time":"2022-04-04T10:08:20Z","timestamp":1649066900000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/cei\/article\/207\/1\/44\/6445821"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,11,29]]},"references-count":20,"journal-issue":{"issue":"1","published-online":{"date-parts":[[2021,11,29]]},"published-print":{"date-parts":[[2022,1,28]]}},"URL":"https:\/\/doi.org\/10.1093\/cei\/uxab026","relation":{},"ISSN":["0009-9104","1365-2249"],"issn-type":[{"value":"0009-9104","type":"print"},{"value":"1365-2249","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2022,1,1]]},"published":{"date-parts":[[2021,11,29]]}}}