{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,16]],"date-time":"2026-05-16T00:49:01Z","timestamp":1778892541257,"version":"3.51.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2022,12,20]],"date-time":"2022-12-20T00:00:00Z","timestamp":1671494400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81774179"],"award-info":[{"award-number":["81774179"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973778"],"award-info":[{"award-number":["81973778"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100017555","name":"Zhejiang University Student Science and Technology Innovation Activity Plan","doi-asserted-by":"publisher","award":["2021R410056"],"award-info":[{"award-number":["2021R410056"]}],"id":[{"id":"10.13039\/501100017555","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,3,16]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Systemic lupus erythematosus (SLE) is a chronic, devastating autoimmune disorder associated with severe organ damage. The roles of Toll-like receptor 9 (TLR9) and NETosis in SLE have been described, suggesting the involvement of NETosis signaling in the development of SLE. Shaoyao-Gancao Decoction (SGT) is a potential medication for the treatment of SLE; however, its potential therapeutic mechanism remains unexplored. To determine the function of SGT in SLE, we treated MRL\/lpr female mice with SGT, the main components of which were paeoniflorin (56.949 \u03bcg\u00b7mL\u22121) and glycyrrhizin (459.393 \u03bcg\u00b7mL\u22121). We found that SGT treatment relieved lymphadenectasis and splenomegaly, reduced urine protein and anti-dsDNA antibody concentrations, and relieved kidney pathology in MRL\/lpr mice. SGT could also effectively regulate the oxidation\/antioxidant balance, significantly reduce malondialdehyde (MDA) and nitric oxide (NO) contents and significantly increase superoxide dismutase (SOD) and glutathione peroxidase (GSH-Px) activities in MRL\/lpr mice. The neutrophil extracellular trap (NET) content of MRL\/lpr mice also decreased to a certain extent after SGT treatment. All these results suggested that SGT might improve the inflammatory damage to tissues caused by oxygen free radicals, thereby regulating the NETosis process mediated by TLR9 and exerting a good therapeutic effect on SLE.<\/jats:p>","DOI":"10.1093\/cei\/uxac119","type":"journal-article","created":{"date-parts":[[2022,12,21]],"date-time":"2022-12-21T02:58:31Z","timestamp":1671591511000},"page":"184-191","source":"Crossref","is-referenced-by-count":5,"title":["Therapeutic effect of Shaoyao-Gancao Decoction on TLR9-mediated NETosis in MRL\/lpr mice"],"prefix":"10.1093","volume":"211","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-7746-1951","authenticated-orcid":false,"given":"Suhai","family":"Qian","sequence":"first","affiliation":[{"name":"School of Basic Medical Sciences, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yibo","family":"Jin","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shate","family":"Xiang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yao","family":"Wang","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zhishan","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Medical Technology and Information, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinghong","family":"Ding","sequence":"additional","affiliation":[{"name":"School of Basic Medical Sciences, Zhejiang Chinese Medical University , Hangzhou , China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2022,12,20]]},"reference":[{"key":"2023031613381139200_","doi-asserted-by":"crossref","first-page":"ITC81","DOI":"10.7326\/AITC202006020","article-title":"Systemic lupus erythematosus","volume":"172","author":"Kiriakidou","year":"2020","journal-title":"Ann Intern Med"},{"key":"2023031613381139200_","doi-asserted-by":"crossref","first-page":"87","DOI":"10.1016\/j.coi.2020.10.014","article-title":"Type I interferon in the pathogenesis of systemic lupus erythematosus","volume":"67","author":"Postal","year":"2020","journal-title":"Curr Opin Immunol"},{"key":"2023031613381139200_","doi-asserted-by":"crossref","first-page":"562","DOI":"10.1097\/BOR.0000000000000738","article-title":"Immunometabolism in the pathogenesis of systemic lupus erythematosus: an update","volume":"32","author":"Romo-Tena","year":"2020","journal-title":"Curr Opin 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injury","volume":"464","author":"Zhang","year":"2010","journal-title":"Nature"},{"key":"2023031613381139200_","doi-asserted-by":"crossref","first-page":"462","DOI":"10.1002\/art.39417","article-title":"Circulating apoptotic microparticles in systemic lupus erythematosus patients drive the activation of dendritic cell subsets and prime neutrophils for NETosis","volume":"68","author":"Dieker","year":"2016","journal-title":"Arthritis Rheumatol"},{"key":"2023031613381139200_","first-page":"120","article-title":"Study on anti-inflammatory effect of Shaoyaogancao decotion.","volume":"37","author":"Zhu","year":"2009","journal-title":"Tianjin Med J"},{"key":"2023031613381139200_","first-page":"581","article-title":"Protective effect of Shaoyaogancao decoction on H2O2-induced oxidative stress injury of PC12 cells.","volume":"35","author":"Zheng","year":"2017","journal-title":"J Guangdong Med Univ"},{"key":"2023031613381139200_","first-page":"723","article-title":"The effect of Shaoyaogancao decoction on CD4 + CD25 + Foxp3 + regulatory T cells in MRL\/lpr mice.","volume":"44","author":"Wang","year":"2009","journal-title":"Zhejiang J Trad Chin Med"},{"key":"2023031613381139200_","first-page":"303","article-title":"The contents of three active components in Shaoyao Glycyrrhiza decoction determined by liquid mass spectrometry.","volume":"52","author":"Xu","year":"2017","journal-title":"Zhejiang J Trad Chin Med"},{"key":"2023031613381139200_","first-page":"33","article-title":"Pathological observation of kidney, spleen and lymph nodes in MRL\/lpr lupus mice","volume":"1","author":"Peng","year":"2008","journal-title":"Lab Anim Comp Med"},{"key":"2023031613381139200_","doi-asserted-by":"crossref","first-page":"833","DOI":"10.1681\/ASN.V104833","article-title":"Mycophenolate mofetil therapy in lupus nephritis: clinical observations","volume":"10","author":"Dooley","year":"1999","journal-title":"J Am Soc 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erythematosus","volume":"66","author":"Carolyne","year":"2014","journal-title":"Arthritis Rheumatol"},{"key":"2023031613381139200_","volume-title":"Experimental study on oxidative antioxidant status in lupus model mice","author":"Jin","year":"2008"}],"container-title":["Clinical and Experimental 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