{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,21]],"date-time":"2026-08-21T14:03:32Z","timestamp":1787321012488,"version":"build-2736575974"},"reference-count":52,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T00:00:00Z","timestamp":1675036800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Italian Group against Systemic Sclerosis"},{"name":"GILS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2023,4,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Anti-centromere (ACA) and antimitochondrial antibodies (AMA) are specific for limited-cutaneous systemic sclerosis (lcSSc) and primary biliary cholangitis (PBC), respectively, and can coexist in up to 25 and 30% of SSc and PBC patients. Here, we evaluated whether anti-centromeric protein A (CENP-A) antibodies cross-react with mitochondrial antigens. To this end, sera from two lcSSc patients (pt1 and pt4), one of them (pt4) also affected by PBC, were used as the source of ACA, previously shown to recognize different groups of amino acids (motifs) in the CENP-A region spanning amino acids 1\u201317 (Ap1\u201317). Pt1 and pt4 Ap1\u201317-specific IgG were purified by affinity-chromatography on insolubilized Ap1\u201317-peptide column and tested by western blotting with nuclear and cytoplasmic protein extract from HeLa cells. Immunoreactive proteins were identified by mass spectrometry and validated by immunodot. The results showed that affinity-purified SSc\/PBC pt4 anti-Ap1\u201317 and not SSc pt1 anti-Ap1\u201317 Ab, specifically cross-reacted with the E2 component of the mitochondrial pyruvate dehydrogenase complex (PDC-E2), the major mitochondrial autoantigen in PBC. Sequence homology analysis indicated that the motif A-x-x-P-x-A-P recognized by pt4 anti-Ap1\u201317 IgG and shared by CENP-A and PDC-E2, is also expressed by some members of the Human Herpesvirus family, suggesting that they may trigger the production of these cross-reacting antibodies.<\/jats:p>","DOI":"10.1093\/cei\/uxad012","type":"journal-article","created":{"date-parts":[[2023,1,30]],"date-time":"2023-01-30T07:21:15Z","timestamp":1675063275000},"page":"32-38","source":"Crossref","is-referenced-by-count":10,"title":["Systemic sclerosis and primary biliary cholangitis share an antibody population with identical specificity"],"prefix":"10.1093","volume":"212","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-4664-1394","authenticated-orcid":false,"given":"Elvira","family":"Favoino","sequence":"first","affiliation":[{"name":"Department of Interdisciplinary Medicine, Rheumatic and Systemic Autoimmune Diseases Unit, University of Bari Medical School , Bari , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ettore","family":"Grapsi","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Medicine, Rheumatic and Systemic Autoimmune Diseases Unit, University of Bari Medical School , Bari , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giovanna","family":"Barbuti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Sciences and Human Oncology, University of Bari Medical School , Bari , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vasiliki","family":"Liakouli","sequence":"additional","affiliation":[{"name":"Department of Precision Medicine, Rheumatology Section, University of Campania \u201cLuigi Vanvitelli\u201d , Naples , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Piero","family":"Ruscitti","sequence":"additional","affiliation":[{"name":"Department of Biotechnological and Applied Clinical Sciences, Rheumatology Unit, University of L\u2019Aquila , L\u2019Aquila , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Caterina","family":"Foti","sequence":"additional","affiliation":[{"name":"Department of Biomedical Science and Human Oncology, Unit of Dermatology, University of Bari Medical School , Bari , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Roberto","family":"Giacomelli","sequence":"additional","affiliation":[{"name":"Department of Medicine, Rheumatology and Immunology Unit, University of Rome \u201cCampus Biomedico\u201d , Rome , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Federico","family":"Perosa","sequence":"additional","affiliation":[{"name":"Department of Interdisciplinary Medicine, Rheumatic and Systemic Autoimmune Diseases Unit, University of Bari Medical School , Bari , 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