{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,23]],"date-time":"2026-08-23T14:48:05Z","timestamp":1787496485409,"version":"build-2736575974"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2023,8,16]],"date-time":"2023-08-16T00:00:00Z","timestamp":1692144000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Uniscientia foundation and an Investigator Initiated Research","award":["IISR-2016-101920"],"award-info":[{"award-number":["IISR-2016-101920"]}]},{"name":"Swiss National Foundation","award":["310030-173240"],"award-info":[{"award-number":["310030-173240"]}]},{"name":"Swiss National Foundation","award":["310030B-192828"],"award-info":[{"award-number":["310030B-192828"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Crohn\u2019s disease (CD) is a chronic immune-mediated disorder of the gastrointestinal tract. Extensive screening studies have revealed the accumulation of immune cell subsets with unique plasticity and immunoregulatory properties in patients with CD. We performed phenotypic and functional studies on inflamed and non-inflamed bioptic tissue to investigate the presence of distinct T cells in the intestinal mucosa of CD patients. We analysed hundreds of surface molecules expressed on cells isolated from the intestinal tissue of CD patients using anti-CD45 mAbs-based barcoding. A gene ontology enrichment analysis showed that proteins that regulate the activation of T cells were the most enriched group. We, therefore, designed T-cell focused multicolour flow-cytometry panels and performed clustering analysis which revealed an accumulation of activated TEM CD4+CD39+ T cells producing IL-17 and IL-21 and increased frequency of terminally differentiated TCR V\u03b41+ cells producing TNF-\u03b1 and IFN-\u03b3 in inflamed tissue of CD patients. The different functional capacities of CD4+ and TCR V\u03b41+ cells in CD lesions indicate their non-overlapping contribution to inflammation. The abnormally high number of terminally differentiated TCR V\u03b41+ cells suggests that they are continuously activated in inflamed tissue, making them a potential target for novel therapies.<\/jats:p>","DOI":"10.1093\/cei\/uxad098","type":"journal-article","created":{"date-parts":[[2023,8,14]],"date-time":"2023-08-14T08:29:02Z","timestamp":1692001742000},"page":"79-93","source":"Crossref","is-referenced-by-count":2,"title":["Surface protein and functional analyses identify CD4+CD39+ TCR \u03b1\u03b2+ and activated TCR V\u03b41+ cells with distinct pro-inflammatory functions in Crohn\u2019s disease lesions"],"prefix":"10.1093","volume":"215","author":[{"given":"Jan","family":"Devan","sequence":"first","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vladimir","family":"Nosi","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"},{"name":"Department of Molecular Biotechnology and Health Sciences, University of Torino , Torino , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julian","family":"Spagnuolo","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Andrew","family":"Chancellor","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aisha","family":"Beshirova","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jose Pedro","family":"Loureiro","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alessandro","family":"Vacchini","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Jan","family":"Hendrik Niess","sequence":"additional","affiliation":[{"name":"Gastroenterology, Department of Biomedicine, University of Basel , Clarunis, Basel , Switzerland"},{"name":"Department of Clinical Research, University of Basel , Basel , Switzerland"},{"name":"University Center for Gastrointestinal and Liver Diseases , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2848-628X","authenticated-orcid":false,"given":"Raffaele","family":"Calogero","sequence":"additional","affiliation":[{"name":"Department of Molecular Biotechnology and Health Sciences, University of Torino , Torino , Italy"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lucia","family":"Mori","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, University Hospital Basel, University of Basel , Basel , Switzerland"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Gennaro","family":"De Libero","sequence":"additional","affiliation":[{"name":"Experimental Immunology, Department of Biomedicine, 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