{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,4,1]],"date-time":"2026-04-01T19:53:47Z","timestamp":1775073227733,"version":"3.50.1"},"reference-count":38,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T00:00:00Z","timestamp":1697068800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>There was evidence that perinuclear antineutrophil cytoplasmic antibodies (pANCA) in autoimmune liver diseases react with human beta-tubulin-5 (TBB5). Here, we reevaluate the specificity and clinical relevance of anti-TBB5 antibodies.<\/jats:p>\n               <jats:p>Patients with untreated autoimmune hepatitis (AIH; n\u2005=\u200553), AIH under immunosuppressive therapy (AIH-IS; n\u2005=\u2005125), primary sclerosing cholangitis (PSC; n\u2005=\u200540), primary biliary cholangitis (PBC; n\u2005=\u2005250), nonautoimmune liver diseases (n\u2005=\u2005158), inflammatory bowel diseases (IBD; n\u2005=\u200530), and healthy individuals (n\u2005=\u200562) were tested by enzyme-linked immunosorbent assay for IgG- and IgA-antibodies against recombinant human TBB5. pANCA were detected by immunofluorescence test. Sera were absorbed with TBB5 coupled to cyanogen bromide-activated sepharose.<\/jats:p>\n               <jats:p>Prevalence and reactivity of IgG anti-TBB5 were significantly higher in patients with untreated AIH (68%; arbitrary units [AU] median: 369) than in PSC (28%; AU median: 84, P\u2005&amp;lt;\u20050.001), other liver diseases (14%; AU median: 185, P\u2005&amp;lt;\u20050.0001), IBD (3%; AU median: 111, P\u2005&amp;lt;\u20050.0001), and healthy controls (3%; AU median: 135; P\u2005&amp;lt;\u20050.0001). Anti-TBB5 did not correlate with pANCA, and immunoprecipitation with TBB5 did not abolish pANCA reactivity. In untreated AIH, anti-TBB5-reactivity was significantly higher than in AIH-IS. Transaminases decreased under IS preferentially in anti-TBB5-negative patients. There was no correlation between anti-TBB5-reactivity and histological stages. IgA-anti-TBB5 was mainly found in alcohol-associated liver disease (ALD; 39%).<\/jats:p>\n               <jats:p>Our data do not support TBB5 as an autoantigenic target of pANCA. However, IgG-anti-TBB5 showed high specificity for (untreated) AIH. While they did not correlate with histological and laboratory parameters, their presence may indicate a poor response to IS.<\/jats:p>","DOI":"10.1093\/cei\/uxad114","type":"journal-article","created":{"date-parts":[[2023,10,12]],"date-time":"2023-10-12T10:31:28Z","timestamp":1697106688000},"page":"146-158","source":"Crossref","is-referenced-by-count":4,"title":["Autoantibodies to beta tubulin in autoimmune liver diseases\u2014Relation to pANCA and clinical relevance"],"prefix":"10.1093","volume":"216","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-9505-707X","authenticated-orcid":false,"given":"Beate","family":"Preu\u00df","sequence":"first","affiliation":[{"name":"Department of Hematology, Oncology, Clinical Immunology and Rheumatology, University of Tuebingen , Tuebingen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Amelie","family":"Frank","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology, Clinical Immunology and Rheumatology, University of Tuebingen , Tuebingen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Birgit","family":"Terjung","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology, GFO Kliniken Bonn, St. Josef Hospital , Bonn , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ulrich","family":"Spengler","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Hepatology, Nephrology, Infectious Diseases, Endocrinology and Diabetology, University of Bonn , Bonn , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Christoph","family":"Berg","sequence":"additional","affiliation":[{"name":"Department of Gastroenterology and Infectiology, University of Tuebingen , Tuebingen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-6111-3901","authenticated-orcid":false,"given":"Reinhild","family":"Klein","sequence":"additional","affiliation":[{"name":"Department of Hematology, Oncology, Clinical Immunology and Rheumatology, University of Tuebingen , Tuebingen , Germany"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,10,12]]},"reference":[{"key":"2024042411063315400_CIT0001","doi-asserted-by":"crossref","first-page":"241","DOI":"10.1055\/s-0029-1233533","article-title":"Autoantibodies and their antigens in autoimmune hepatitis","volume":"29","author":"Bogdanos","year":"2009","journal-title":"Semin Liver Dis"},{"key":"2024042411063315400_CIT0002","doi-asserted-by":"crossref","first-page":"802","DOI":"10.1016\/j.dld.2011.05.009","article-title":"Immune-mediated diseases in primary sclerosing 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