{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,19]],"date-time":"2026-08-19T12:06:19Z","timestamp":1787141179016,"version":"build-2736575974"},"reference-count":30,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2023,12,8]],"date-time":"2023-12-08T00:00:00Z","timestamp":1701993600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001411","name":"Indian council of Medical Research","doi-asserted-by":"publisher","award":["45\/08\/2022-IMM\/BMS"],"award-info":[{"award-number":["45\/08\/2022-IMM\/BMS"]}],"id":[{"id":"10.13039\/501100001411","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,4,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Atopic dermatitis (AD) is a persistent and recurring inflammatory condition affecting the skin. An expanding corpus of evidence indicates the potential participation of transforming growth factor-\u03b21 (TGF-\u03b21) in the modulation of inflammation and tissue remodeling in AD. The primary objective of this study was to examine the aberrant modulation of TGF-\u03b21\/small mothers against decapentaplegic homolog 3 (SMAD3) signaling through a comprehensive analysis of their molecular and protein expression profiles. The study encompassed an aggregate of 37 participants, which included 25 AD patients and 12 controls. The assessment of mRNA and protein levels of TGF-\u03b21 and SMAD3 was conducted utilizing quantitative real-time PCR and immunohistochemistry (IHC), whereas serum IgE and vitamin D levels were estimated by ELISA and chemiluminescence, respectively. Quantitative analysis demonstrated a 2.5-fold upregulation of TGF-\u03b21 mRNA expression in the lesional AD skin (P\u2005&amp;lt;\u20050.0001). IHC also exhibited a comparable augmented pattern, characterized by moderate to strong staining intensities. In addition, TGF-\u03b21 mRNA showed an association with vitamin D deficiency in serum (P\u2005&amp;lt;\u20050.02), and its protein expression was linked with the disease severity (P\u2005&amp;lt;\u20050.01) Furthermore, a significant decrease in the expression of the SMAD3 gene was observed in the affected skin (P\u2005=\u20050.0004). This finding was further confirmed by evaluating the protein expression and phosphorylation of SMAD3, both of which exhibited a decrease. These findings suggest that there is a dysregulation in the TGF-\u03b21\/SMAD3 signaling pathway in AD. Furthermore, the observed augmentation in mRNA and protein expression of TGF-\u03b21, along with its correlation with the disease severity, holds considerable clinical significance and emphasizes its potential role in AD pathogenesis.<\/jats:p>","DOI":"10.1093\/cei\/uxad130","type":"journal-article","created":{"date-parts":[[2023,12,9]],"date-time":"2023-12-09T04:22:53Z","timestamp":1702095773000},"page":"192-199","source":"Crossref","is-referenced-by-count":14,"title":["Investigating dysregulation of TGF-\u03b21\/SMAD3 signaling in atopic dermatitis: a molecular and immunohistochemical analysis"],"prefix":"10.1093","volume":"216","author":[{"given":"Tabasum","family":"Shafi","sequence":"first","affiliation":[{"name":"Department of Immunology and Molecular Medicine, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3898-0158","authenticated-orcid":false,"given":"Roohi","family":"Rasool Wani","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Medicine, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Showkat","family":"Hussain","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Medicine, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Imtiyaz A","family":"Bhat","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Medicine, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rumana","family":"Makhdoomi","sequence":"additional","affiliation":[{"name":"Department of Pathology, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Sheikh Adil","family":"Bashir","sequence":"additional","affiliation":[{"name":"Department of Plastic and Reconstructive Surgery, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Iffat","family":"Hassan","sequence":"additional","affiliation":[{"name":"Department of Dermatology, Venereology, and Leprosy, GMC , Srinagar , India- 190010"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zafar A","family":"Shah","sequence":"additional","affiliation":[{"name":"Department of Immunology and Molecular Medicine, SKIMS , Srinagar , India- 190011"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2023,12,8]]},"reference":[{"key":"2024042411060367100_CIT0001","doi-asserted-by":"crossref","first-page":"304","DOI":"10.1111\/bjd.19580","article-title":"The global burden of atopic dermatitis: lessons from the Global Burden of Disease Study 1990\u20132017","volume":"184","author":"Laughter","year":"2021","journal-title":"Br J Dermatol"},{"key":"2024042411060367100_CIT0002","doi-asserted-by":"crossref","first-page":"1136","DOI":"10.1056\/NEJMra2023911","article-title":"Atopic dermatitis","volume":"384","author":"St\u00e4nder","year":"2021","journal-title":"N Engl J Med"},{"key":"2024042411060367100_CIT0003","doi-asserted-by":"crossref","first-page":"345","DOI":"10.1016\/S0140-6736(20)31286-1","article-title":"Atopic dermatitis","volume":"396","author":"Langan","year":"2020","journal-title":"Lancet"},{"key":"2024042411060367100_CIT0004","doi-asserted-by":"crossref","first-page":"144","DOI":"10.1016\/j.anai.2019.04.020","article-title":"Comorbidities and the impact of atopic dermatitis","volume":"123","author":"Silverberg","year":"2019","journal-title":"Ann Allergy Asthma Immunol"},{"key":"2024042411060367100_CIT0005","doi-asserted-by":"crossref","first-page":"74","DOI":"10.1007\/s11882-020-00971-z","article-title":"The role of environmental exposures in atopic dermatitis","volume":"20","author":"Narla","year":"2020","journal-title":"Curr Allergy Asthma Rep"},{"key":"2024042411060367100_CIT0006","doi-asserted-by":"crossref","first-page":"274","DOI":"10.1016\/j.jid.2020.07.006","article-title":"Which way do we go? 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