{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,5]],"date-time":"2026-10-05T23:30:43Z","timestamp":1791243043479,"version":"4.1.0"},"reference-count":48,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T00:00:00Z","timestamp":1705881600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Healthcare Institute Carlos III","award":["PI06\/730"],"award-info":[{"award-number":["PI06\/730"]}]},{"name":"Spanish Ministry of Education and Science","award":["CAS14\/00311"],"award-info":[{"award-number":["CAS14\/00311"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,6,20]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>Crohn\u2019s disease (CD) is a chronic relapsing inflammatory disorder in which defective apoptosis of mucosal T cells is postulated to produce sustained inflammation and reactive oxygen species accumulation. Whether CD T cells are intrinsically resistant to apoptosis or whether this resistance is acquired at the intestinal site needs to be clarified, as the cellular mechanisms modulate the impaired apoptosis in these cells. Here, we analysed peripheral blood T cells from patients na\u00efve to specific CD treatment at the onset and from healthy controls. Non-activated freshly purified lymphocytes were cultured and submitted to in vitro protocols for activation (CD3\/CD28 antibodies) and apoptosis (Fas antibody). Cells were analysed by flow cytometry. Caspases (3, 8, and 9) and catalase activity were measured; protein levels of bax, Bcl-2, and NF-kB were detected by western blotting, and cytokines by Luminex-based assays. The results showed that CD4 T cells from CD patients are less prone to apoptosis before they can migrate to the intestinal mucosa. Caspase-9, FasR, sIL-2R\u03b1, IL-17A, IFN\u03b3, IL-6, TNF-\u03b1, and IL-10 were shown to be significantly different in CD but not for the rest of the analysed biological elements. Catalase activity was significantly reduced in CD T cells, which was confirmed in ex vivo experiments in which catalase inhibition in T cells from healthy controls triggered apoptosis inhibition in a dose-dependent manner. In conclusion, apoptosis inhibition of CD T cells is a feature of these cells before they can migrate to the intestinal mucosa. Noteworthy, the impaired apoptosis of T cells can be directly influenced by catalase inhibition.<\/jats:p>","DOI":"10.1093\/cei\/uxad134","type":"journal-article","created":{"date-parts":[[2024,1,22]],"date-time":"2024-01-22T09:19:45Z","timestamp":1705915185000},"page":"45-56","source":"Crossref","is-referenced-by-count":8,"title":["Catalase inhibition can modulate the ability of peripheral blood T cells to undergo apoptosis in Crohn\u2019s disease"],"prefix":"10.1093","volume":"217","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2086-1521","authenticated-orcid":false,"given":"In\u00e9s","family":"Moret-Tatay","sequence":"first","affiliation":[{"name":"Inflammatory Bowel Disease Research Group, Health Research Institute, Hospital La Fe (IIS La Fe) , Valencia , Spain"},{"name":"General Directorate of Public Health, Council of Healthcare , Valencia , Spain"},{"name":"Biomedical Research Centre, Hepatic and Digestive Diseases Network (Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas [CIBEREHD]) , Madrid , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Pilar","family":"Nos","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Hepatic and Digestive Diseases Network (Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas [CIBEREHD]) , Madrid , Spain"},{"name":"Gastroenterology Unit, La Fe University and Polytechnic Hospital , Valencia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marisa","family":"Iborra","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Hepatic and Digestive Diseases Network (Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas [CIBEREHD]) , Madrid , Spain"},{"name":"Gastroenterology Unit, La Fe University and Polytechnic Hospital , Valencia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francisco","family":"Rausell","sequence":"additional","affiliation":[{"name":"Inflammatory Bowel Disease Research Group, Health Research Institute, Hospital La Fe (IIS La Fe) , Valencia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Bel\u00e9n","family":"Beltr\u00e1n","sequence":"additional","affiliation":[{"name":"Biomedical Research Centre, Hepatic and Digestive Diseases Network (Centro de Investigaci\u00f3n Biom\u00e9dica en Red de Enfermedades Hep\u00e1ticas y Digestivas [CIBEREHD]) , Madrid , Spain"},{"name":"Gastroenterology Unit, La Fe University and Polytechnic Hospital , Valencia , Spain"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,1,22]]},"reference":[{"key":"2024062013073053700_CIT0001","doi-asserted-by":"crossref","first-page":"1081","DOI":"10.4049\/jimmunol.163.2.1081","article-title":"Resistance of Crohn\u2019s 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