{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T08:27:51Z","timestamp":1740126471485,"version":"3.37.3"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2024,2,4]],"date-time":"2024-02-04T00:00:00Z","timestamp":1707004800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Cancer Center of Eastern Finland"},{"DOI":"10.13039\/100007753","name":"University of Eastern Finland","doi-asserted-by":"publisher","id":[{"id":"10.13039\/100007753","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100004092","name":"Kuopio University Hospital","doi-asserted-by":"publisher","id":[{"id":"10.13039\/501100004092","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Psoriasis is a chronic inflammatory skin disease with a characteristic isomorphic reaction, i.e. the K\u00f6bner reaction, induced by slight epidermal trauma. In this study, the tape-stripping technique was used to induce the development of K\u00f6bner reaction in 18 subjects with psoriasis. Eight subjects developed a positive reaction. To study the early cellular changes, skin biopsies were taken at the baseline and subsequent time points of 2 h, 1 d, 3 d, and 7 d for the immunostaining of complement C3c, iC3b, and cells expressing complement receptor 3 (CD11b\/CD18; a receptor of iC3b) or CD14. The results show that the positive K\u00f6bner reaction is associated with rapid (2 h\u20131 d) and sustained (3\u20137 d) increase in the expression of epidermal C3c and iC3b and dermal C3c. In addition, there was a positive correlation between CD11b+ and CD14+ cells in baseline and 2 h\u20131 d biopsies with a subsequent increase in CD11b+ and CD14+ cells in 3\u20137 d biopsies in the K\u00f6bner-positive group. In the K\u00f6bner-negative group, only a transient increase in epidermal iC3b at 2 h\u20131 d, as well as rapid (2 h\u20131 d) and sustained increase (3\u20137 d) in dermal iC3b and CD14+ cells, was observed. In experiments with cultured monolayer keratinocytes, a slight cell damage already at 30 mJ\/cm2 ultraviolet B irradiation led to increased expression of C3c, but not iC3b. Therefore, there are marked differences between K\u00f6bner groups in respect to the expression of C3c, iC3b, and cells expressing CD11b or CD14. Of note is the rapid and sustained increase in epidermal C3c and iC3b in the positive K\u00f6bner reaction.<\/jats:p>","DOI":"10.1093\/cei\/uxae009","type":"journal-article","created":{"date-parts":[[2024,2,2]],"date-time":"2024-02-02T12:56:46Z","timestamp":1706878606000},"page":"252-261","source":"Crossref","is-referenced-by-count":0,"title":["Increased expression of complement C3c, iC3b, and cells containing CD11b or CD14 in experimentally induced psoriatic lesion"],"prefix":"10.1093","volume":"216","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2528-3898","authenticated-orcid":false,"given":"Dina","family":"Rahkola","sequence":"first","affiliation":[{"name":"Department of Dermatology, University of Eastern Finland and Kuopio University Hospital , Kuopio , Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0076-9093","authenticated-orcid":false,"given":"Rauno J","family":"Harvima","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Eastern Finland and Kuopio University Hospital , Kuopio , Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9504-7255","authenticated-orcid":false,"given":"Ilkka T","family":"Harvima","sequence":"additional","affiliation":[{"name":"Department of Dermatology, University of Eastern Finland and Kuopio University Hospital , Kuopio , Finland"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,2,4]]},"reference":[{"key":"2024051613325906400_CIT0001","doi-asserted-by":"crossref","first-page":"779","DOI":"10.1111\/j.1600-0625.2007.00629.x","article-title":"Immunopathogenesis of psoriasis","volume":"16","author":"Sabat","year":"2007","journal-title":"Exp 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