{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,9,4]],"date-time":"2026-09-04T06:39:59Z","timestamp":1788503999996,"version":"build-2803163510"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T00:00:00Z","timestamp":1721606400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/creativecommons.org\/licenses\/by\/4.0\/"}],"funder":[{"name":"Fyrbodal Research and Development Council Region V\u00e4stra G\u00f6taland"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,9,16]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Eosinophilic esophagitis (EoE) is a chronic Th2-mediated inflammatory disease of the esophagus driven by dietary or inhalant allergens which if left untreated, leads to fibrosis and poor esophageal function. Although the inflammation in the esophagus is dominated by eosinophils, there are also elevated levels of T and B cells. Blood samples from ten patients with EoE before and after treatment with orodispersible budesonide and 10 healthy controls were compared using cytometry by time-of-flight. An antibody panel was designed that covers the major immunological cell populations with a particular focus on eosinophils. The data was analyzed with multivariate methods and cluster analysis. Correlation analysis was done between immune markers and endoscopic, histological, and symptomatologic assessments. Our analysis revealed that patients with EoE had lower levels of effector memory T cells after treatment with orodispersible budesonide to the same level as healthy subjects. In addition, more suppressive eosinophils were present in the circulation of EoE patients before treatment and more immature eosinophils were present after treatment. Furthermore, levels of galectin-10+ eosinophils correlated with histological findings in esophageal tissue from EoE patients. In all patients, the peak eosinophils were decreased after treatment with orodispersible budesonide. Intriguingly, 90% of the patients had remission in the histological assessment and 50% improved in the endoscopic assessment. This study reports a detailed immune profile in patients with EoE before and after treatment with orodispersible budesonide and it is a step toward finding blood-based immune parameters that could be useful to monitor response to treatment.<\/jats:p>","DOI":"10.1093\/cei\/uxae065","type":"journal-article","created":{"date-parts":[[2024,7,22]],"date-time":"2024-07-22T06:27:41Z","timestamp":1721629661000},"page":"1-13","source":"Crossref","is-referenced-by-count":1,"title":["Investigating immune profile by CyTOF in patients with eosinophilic esophagitis after treatment with orodispersible budesonide"],"prefix":"10.1093","volume":"218","author":[{"ORCID":"https:\/\/orcid.org\/0009-0009-1988-2561","authenticated-orcid":false,"given":"John","family":"Plate","sequence":"first","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery, Region V\u00e4stra G\u00f6taland, NU-Hospital Group , Trollh\u00e4ttan , Sweden"},{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-2038-4286","authenticated-orcid":false,"given":"Sofie","family":"Albinsson H\u00f6gberg","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Infectious Diseases, The Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hardis","family":"Rabe","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Infectious Diseases, The Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden"},{"name":"RISE Research Institutes of Sweden, Bioscience and Materials , Gothenburg , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Helen","family":"Larsson","sequence":"additional","affiliation":[{"name":"Department of Otorhinolaryngology Head and Neck Surgery, Region V\u00e4stra G\u00f6taland, NU-Hospital Group , Trollh\u00e4ttan , Sweden"},{"name":"Department of Otorhinolaryngology, Head and Neck Surgery, Institute of Clinical Sciences, Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-1284-6911","authenticated-orcid":false,"given":"Christine","family":"Lingblom","sequence":"additional","affiliation":[{"name":"Institute of Biomedicine, Department of Infectious Diseases, The Sahlgrenska Academy, University of Gothenburg , Gothenburg , Sweden"},{"name":"Department of Clinical Microbiology, Sahlgrenska University Hospital , Gothenburg , 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