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The purpose of this study is to investigate whether low-intensity pulsed ultrasound stimulation (PUS) inhibits CSD by modulating neural activity and hemodynamics. Behavioral test, intrinsic signal optical imaging and western blot analysis were used for evaluating the inhibition effect of PUS on CSD in rat. We found that: 1) 30\u00a0min of PUS can significantly improve motor activity of rat with CSD. 2) Both 30\u00a0s and 30\u00a0min of PUS can significantly reduce count and propagation speed of CSD in rat and the inhibitory effect was enhanced with increase of ultrasound intensity. 3) 30\u00a0min of PUS significantly enhanced levels of brain-derived neurotrophic factor protein in brain tissue with CSD. These results suggest that PUS has the potential to treat brain disorders associated with CSD.<\/jats:p>","DOI":"10.1093\/cercor\/bhab055","type":"journal-article","created":{"date-parts":[[2021,2,24]],"date-time":"2021-02-24T12:16:56Z","timestamp":1614169016000},"page":"3872-3880","source":"Crossref","is-referenced-by-count":12,"title":["Low-Intensity Pulsed Ultrasound Stimulation Inhibits Cortical Spreading Depression"],"prefix":"10.1093","volume":"31","author":[{"given":"Nannan","family":"Bian","sequence":"first","affiliation":[{"name":"Institute of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Yuan","sequence":"additional","affiliation":[{"name":"Institute of Electrical Engineering, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yingwei","family":"Li","sequence":"additional","affiliation":[{"name":"Institute of Information Engineering, Yanshan University, Qinhuangdao 066004, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mengyang","family":"Liu","sequence":"additional","affiliation":[{"name":"Center for Medical Physics and Biomedical Engineering, Medical University of Vienna, Vienna 1090, Austria"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiaoli","family":"Li","sequence":"additional","affiliation":[{"name":"State Key Laboratory of Cognitive Neuroscience and Learning, Beijing Normal University, Beijing 100875, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,4,15]]},"reference":[{"key":"2021070818253819900_ref1","doi-asserted-by":"crossref","first-page":"2002","DOI":"10.1093\/brain\/aww118","article-title":"Transcranial magnetic stimulation and potential cortical and trigeminothalamic mechanisms in 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