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Subplate neurons (SPNs) are among the first-born cortical neurons and are necessary for normal cerebral development. While moderate or severe HI at P1 in rats leads to SPN loss, it is unclear if HI, esp. forms not associated with overt cell loss lead to altered SPN circuits. Thus, we used two HI models with different severities in P1 rats. Cauterization of the common carotid artery (CCA) causes a largely transient and thus milder ischemia (HI-Caut) while CCA ligation causes more severe ischemia (HI-Lig). While HI-Lig caused subplate damage, HI-Caut did not cause overt histological damage on the light microscopic level. We used laser-scanning photostimulation (LSPS) in acute thalamocortical slices of auditory cortex during P5\u201310 to study the functional connectivity of SPNs. Both HI categories resulted in hyperconnectivity of excitatory and inhibitory circuits to SPNs. Thus, alterations on the circuit level are present in the absence of cell loss. Our results show that SPN circuits are uniquely susceptible to HI. Given the key developmental role of SPNs, our results suggest that altered SPN circuits might underlie the abnormal development of cortical function after HI.<\/jats:p>","DOI":"10.1093\/cercor\/bhx358","type":"journal-article","created":{"date-parts":[[2017,12,21]],"date-time":"2017-12-21T22:34:44Z","timestamp":1513895684000},"page":"765-776","source":"Crossref","is-referenced-by-count":41,"title":["Neonatal Hypoxia\u2013Ischemia Causes Functional Circuit Changes in Subplate Neurons"],"prefix":"10.1093","volume":"29","author":[{"given":"Aminah","family":"Sheikh","sequence":"first","affiliation":[{"name":"Department of Biology, University of Maryland, College Park, MD, USA"},{"name":"Neuroscience and Cognitive Science Program, University of Maryland, College Park, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiangying","family":"Meng","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Maryland, College Park, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ji","family":"Liu","sequence":"additional","affiliation":[{"name":"Department of Biology, University of Maryland, College Park, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Alexandra","family":"Mikhailova","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Neurology, University of California, San Francisco, CA, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Joseph P Y","family":"Kao","sequence":"additional","affiliation":[{"name":"Center for Biomedical Engineering and Technology, and Department of Physiology, University of Maryland School of Medicine, Baltimore, MD, USA"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Patrick S","family":"McQuillen","sequence":"additional","affiliation":[{"name":"Departments of Pediatrics and Neurology, University of California, San Francisco, CA, 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