{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T10:29:13Z","timestamp":1786530553220,"version":"build-2736575974"},"reference-count":19,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2026,1,1]],"date-time":"2026-01-01T00:00:00Z","timestamp":1767225600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/501100001691","name":"Grants-in-Aid for Scientific Research","doi-asserted-by":"crossref","id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"crossref"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["22K11890"],"award-info":[{"award-number":["22K11890"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001691","name":"Japan Society for the Promotion of Science","doi-asserted-by":"publisher","award":["25K14924"],"award-info":[{"award-number":["25K14924"]}],"id":[{"id":"10.13039\/501100001691","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Public Interest Foundation for the Vitamin & Biofactor Society","award":["2024"],"award-info":[{"award-number":["2024"]}]},{"name":"Vitamin C Research Committee of Japan","award":["2025"],"award-info":[{"award-number":["2025"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,3,2]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sodium ascorbate (AsA-Na) is widely used as a source of vitamin C. However, the mechanisms regulating voluntary intake across different concentrations and nutritional states remain poorly understood. Therefore, we examined behavioral and neural responses to AsA-Na in osteogenic disorder Shionogi (ODS) od\/od rats, which lack endogenous vitamin C synthesis. Two-bottle choice tests were used to evaluate long-term behavioral responses, and the contribution of epithelial sodium channel (ENaC)-dependent taste signaling was examined using amiloride. In parallel, chorda tympani (CT) nerve responses were recorded with and without amiloride treatment. AsA-Na consistently showed lower preference scores than NaCl across concentrations. At 30 to 100\u2005mM, the preference for AsA-Na was lower than that for NaCl but significantly higher than that for AsA. Lick responses to AsA-Na were comparable to those for NaCl and were enhanced under vitamin C\u2013deficient conditions. Although the 48-h intake of 100\u2005mM AsA-Na remained low under all conditions, the intake of 10\u2005mM AsA-Na increased selectively during the re-intake phase. Notably, amiloride strongly suppressed CT nerve responses to AsA-Na. While amiloride significantly reduced NaCl preference and intake, it had no effect on AsA-Na preference or sustained intake. These results support a dissociation between ENaC-mediated peripheral sensory signaling and long-term ingestive behavior. Although AsA-Na is detected via sodium taste pathways, this sensory input alone cannot account for sustained intake. Instead, intake is likely influenced by post-ingestive mechanisms in a concentration- and state-dependent manner. These findings suggest an important contribution of internal nutritional signals in shaping chemosensory-driven behavior.<\/jats:p>","DOI":"10.1093\/chemse\/bjag024","type":"journal-article","created":{"date-parts":[[2026,7,28]],"date-time":"2026-07-28T11:14:40Z","timestamp":1785237280000},"source":"Crossref","is-referenced-by-count":0,"title":["Dissociation between amiloride-sensitive taste responses and ingestive behavior to sodium ascorbate in osteogenic disorder Shionogi rats"],"prefix":"10.1093","volume":"51","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0575-7582","authenticated-orcid":false,"given":"Toshiaki","family":"Yasuo","sequence":"first","affiliation":[{"name":"Department of Oral Physiology, Asahi University School of Dentistry , Mizuho, Gifu 501-0296 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-4444-5103","authenticated-orcid":false,"given":"Shinpei","family":"Takahashi","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, Asahi University School of Dentistry , Mizuho, Gifu 501-0296 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8655-3471","authenticated-orcid":false,"given":"Shusuke","family":"Iwata","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, Asahi University School of Dentistry , Mizuho, Gifu 501-0296 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0009-0001-2194-2748","authenticated-orcid":false,"given":"Noritaka","family":"Sako","sequence":"additional","affiliation":[{"name":"Department of Oral Physiology, Asahi University School of Dentistry , Mizuho, Gifu 501-0296 ,","place":["Japan"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2026,7,29]]},"reference":[{"issue":"5634","key":"2026081205161475400_bjag024-B1","doi-asserted-by":"publisher","first-page":"850","DOI":"10.1126\/science.1087155","article-title":"Detection of sweet and umami taste in the absence of taste receptor T1r3","volume":"301","author":"Damak","year":"2003","journal-title":"Science"},{"issue":"1 Pt 2","key":"2026081205161475400_bjag024-B2","doi-asserted-by":"publisher","first-page":"R52","DOI":"10.1152\/ajpregu.1985.249.1.R52","article-title":"Analysis of amiloride inhibition of chorda tympani taste response of rat to NaCl","volume":"249","author":"Desimone","year":"1985","journal-title":"Am J Physiol."},{"issue":"2_suppl","key":"2026081205161475400_bjag024-B3","doi-asserted-by":"publisher","first-page":"51","DOI":"10.1080\/10915810590953851","article-title":"Final report of the safety assessment of l-ascorbic acid, calcium ascorbate, magnesium ascorbate, magnesium ascorbyl phosphate, sodium ascorbate, and sodium ascorbyl phosphate as used in cosmetics","volume":"24","author":"Elmore","year":"2005","journal-title":"Int J Toxicol"},{"issue":"12","key":"2026081205161475400_bjag024-B4","doi-asserted-by":"publisher","first-page":"1630","DOI":"10.1093\/jn\/115.12.1630","article-title":"Requirement for ascorbic acid in a rat mutant unable to synthesize ascorbic acid","volume":"115","author":"Horio","year":"1985","journal-title":"J Nutr"},{"issue":"20","key":"2026081205161475400_bjag024-B5","doi-asserted-by":"publisher","first-page":"11044","DOI":"10.1073\/pnas.190066697","article-title":"Leptin as a modulator of sweet taste sensitivities in mice","volume":"97","author":"Kawai","year":"2000","journal-title":"Proc Natl Acad Sci U S A."},{"issue":"2","key":"2026081205161475400_bjag024-B6","doi-asserted-by":"publisher","first-page":"309","DOI":"10.1016\/S0006-8993(96)01118-3","article-title":"Temperature and amiloride alter taste nerve responses to Na+, K+, and NH4+ salts in rats","volume":"744","author":"Lundy","year":"1997","journal-title":"Brain Res"},{"issue":"2","key":"2026081205161475400_bjag024-B7","doi-asserted-by":"publisher","first-page":"507","DOI":"10.1016\/S0031-9384(96)80026-6","article-title":"Amiloride inhibits taste nerve responses to NaCl and KCl in Sprague-Dawley and Fischer 344 rats","volume":"60","author":"Minear","year":"1996","journal-title":"Physiol Behav"},{"issue":"4","key":"2026081205161475400_bjag024-B8","doi-asserted-by":"publisher","first-page":"359","DOI":"10.1007\/BF01952551","article-title":"A rat mutant unable to synthesize vitamin C","volume":"40","author":"Mizushima","year":"1984","journal-title":"Experientia"},{"issue":"9","key":"2026081205161475400_bjag024-B9","doi-asserted-by":"publisher","first-page":"5347","DOI":"10.1073\/pnas.95.9.5347","article-title":"Reinnervation of cross-regenerated gustatory nerve fibers into amiloride-sensitive and amiloride-insensitive taste receptor cells","volume":"95","author":"Ninomiya","year":"1998","journal-title":"Proc Natl Acad Sci U S A."},{"issue":"1\u20132","key":"2026081205161475400_bjag024-B10","doi-asserted-by":"publisher","first-page":"319","DOI":"10.1016\/0006-8993(88)90777-9","article-title":"Amiloride inhibition of responses of rat single chorda tympani fibers to chemical tongue stimulations","volume":"451","author":"Ninomiya","year":"1988","journal-title":"Brain Res"},{"issue":"2","key":"2026081205161475400_bjag024-B11","doi-asserted-by":"publisher","first-page":"370","DOI":"10.1016\/0006-8993(82)90100-7","article-title":"Gustatory neural response in the mouse","volume":"244","author":"Ninomiya","year":"1982","journal-title":"Brain Res"},{"issue":"6","key":"2026081205161475400_bjag024-B12","doi-asserted-by":"publisher","first-page":"565","DOI":"10.1093\/chemse\/bjj061","article-title":"Amiloride inhibition on NaCl responses of the chorda tympani nerve in two 129 substrains of mice, 129P3\/J and 129X1\/SvJ","volume":"31","author":"Ohkuri","year":"2006","journal-title":"Chem Senses"},{"issue":"3","key":"2026081205161475400_bjag024-B13","doi-asserted-by":"crossref","first-page":"261","DOI":"10.1093\/chemse\/28.3.261","article-title":"Synergistic responses of the chorda tympani to mixtures of umami and sweet substances in rats","volume":"28","author":"Sako","year":"2003","journal-title":"Chem Senses"},{"issue":"1\u20132","key":"2026081205161475400_bjag024-B14","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/S0304-3940(99)00019-1","article-title":"Analyses of taste nerve responses with special reference to possible receptor mechanisms of umami taste in the rat","volume":"261","author":"Sako","year":"1999","journal-title":"Neurosci Lett"},{"issue":"15","key":"2026081205161475400_bjag024-B15","doi-asserted-by":"publisher","first-page":"6267","DOI":"10.1523\/JNEUROSCI.5599-12.2013","article-title":"Angiotensin II modulates salty and sweet taste sensitivities","volume":"33","author":"Shigemura","year":"2013","journal-title":"J Neurosci"},{"issue":"7070","key":"2026081205161475400_bjag024-B16","doi-asserted-by":"publisher","first-page":"1022","DOI":"10.1038\/nature04248","article-title":"Heat activation of TRPM5 underlies thermal sensitivity of sweet taste","volume":"438","author":"Talavera","year":"2005","journal-title":"Nature"},{"issue":"1","key":"2026081205161475400_bjag024-B17","doi-asserted-by":"publisher","first-page":"3","DOI":"10.1007\/s00424-020-02464-4","article-title":"Taste transduction and channel synapses in taste buds","volume":"473","author":"Taruno","year":"2021","journal-title":"Pflugers Arch"},{"key":"2026081205161475400_bjag024-B18","doi-asserted-by":"publisher","DOI":"10.1093\/chemse\/bjad008","article-title":"Vitamin C deficiency in osteogenic disorder Shionogi\/Shi JCL-od\/od rats: effects on sour taste preferences, lick rates, chorda tympani nerve responses, and taste transduction elements","volume":"48","author":"Yasuo","year":"2023","journal-title":"Chem Senses"},{"issue":"6","key":"2026081205161475400_bjag024-B19","doi-asserted-by":"publisher","first-page":"389","DOI":"10.1093\/chemse\/bjz028","article-title":"Behavioral and neural responses to vitamin C solution in vitamin C-deficient osteogenic disorder Shionogi\/Shi Jcl-od\/od rats","volume":"44","author":"Yasuo","year":"2019","journal-title":"Chem Senses"}],"container-title":["Chemical Senses"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/chemse\/advance-article-pdf\/doi\/10.1093\/chemse\/bjag024\/70358748\/bjag024.pdf","content-type":"application\/pdf","content-version":"am","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/chemse\/article-pdf\/doi\/10.1093\/chemse\/bjag024\/70358748\/bjag024.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/chemse\/article-pdf\/doi\/10.1093\/chemse\/bjag024\/70358748\/bjag024.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2026,8,12]],"date-time":"2026-08-12T09:35:27Z","timestamp":1786527327000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/chemse\/article\/doi\/10.1093\/chemse\/bjag024\/8747003"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2026]]},"references-count":19,"URL":"https:\/\/doi.org\/10.1093\/chemse\/bjag024","relation":{},"ISSN":["0379-864X","1464-3553"],"issn-type":[{"value":"0379-864X","type":"print"},{"value":"1464-3553","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2026]]},"published":{"date-parts":[[2026]]},"article-number":"bjag024"}}