{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,7,19]],"date-time":"2026-07-19T14:19:49Z","timestamp":1784470789850,"version":"3.55.0"},"reference-count":42,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2020,2,26]],"date-time":"2020-02-26T00:00:00Z","timestamp":1582675200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,4,25]]},"abstract":"<jats:title>Abstract<\/jats:title><jats:p>A simple, rapid, cost-effective and green analytical method is developed based on ultrasound-assisted dispersive liquid\u2013liquid microextraction (US-DLLME) coupled to thin-layer chromatography (TLC)-image analysis for the simultaneous determination of two major alkaloids of Strychnos nux-vomica L i.e., strychnine and brucine. The method is composed of three steps, namely (i) US-DLLME by injecting a mixture of 100-\u03bcL chloroform (extraction solvent) and 1-mL methanol (disperser solvent) in 5\u00a0mL of aqueous sample, followed by ultrasonication and centrifugation, (ii) TLC of 20\u00a0\u03bcL of sedimented phase with methanol: ammonia (100:1.5, v\/v) as the mobile phase and visualization under ultraviolet radiation (254\u00a0nm) and (iii) photography of TLC plate and quantification of spots by image analysis using freely available imageJ software (National Institute of Health, Bethesda, MD, USA). The limit of detection and limit of quantification for both alkaloids were found to be in the range of 0.12\u20130.15 and 0.36\u20130.48\u00a0\u03bcg\/spot, respectively. The method was found to be linear in the range of 0.5\u20135\u00a0\u03bcg\/spot with correlation coefficient (R2) of 0.995 and 0.997 for strychnine and brucine, respectively. The developed method was successfully applied for the determination of strychnine and brucine in Ayurvedic formulations and blood samples. The method does not require any sophisticated instrument and handling skills and can be adopted for rapid analysis of strychnine and brucine in forensic toxicological laboratories.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaa007","type":"journal-article","created":{"date-parts":[[2020,1,28]],"date-time":"2020-01-28T12:13:43Z","timestamp":1580213623000},"page":"477-484","source":"Crossref","is-referenced-by-count":16,"title":["A simple, cost-effective and rapid method for simultaneous determination of Strychnos nux-vomica alkaloids in blood and Ayurvedic medicines based on ultrasound-assisted dispersive liquid\u2013liquid microextraction\u2013thin-layer chromatography-image analysis"],"prefix":"10.1093","volume":"58","author":[{"given":"Rajeev","family":"Jain","sequence":"first","affiliation":[{"name":"Forensic Toxicology Division, Central Forensic Science Laboratory, Urput-Kumeria Road, P.O. Maniari Tiniali, Kamrup (Rural), Assam 781125, India"},{"name":"Forensic Toxicology Division, Central Forensic Science Laboratory, Plot #2, Sector 36-A, Dakshin Marg, Chandigarh 160036, India, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Rohitashva Mani","family":"Tripathi","sequence":"first","affiliation":[{"name":"Forensic Toxicology Division, Central Forensic Science Laboratory, Plot #2, Sector 36-A, Dakshin Marg, Chandigarh 160036, India, and"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Archna","family":"Negi","sequence":"first","affiliation":[{"name":"Forensic Chemistry Division, Central Forensic Science Laboratory, Plot #2, Sector 36-A, Dakshin Marg, Chandigarh 160036, India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Shishir Pratap","family":"Singh","sequence":"first","affiliation":[{"name":"Forensic Toxicology Division, Central Forensic Science Laboratory, Urput-Kumeria Road, P.O. Maniari Tiniali, Kamrup (Rural), Assam 781125, India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,2,26]]},"reference":[{"key":"2020042702555697400_ref1","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.jchromb.2015.11.012","article-title":"LC-MS\/MS determination and comparative pharmacokinetics of strychnine, brucine and their metabolites in rat plasma after intragastric administration of each monomer and the total alkaloids for semen strychnine","volume":"1008","author":"Lin","year":"2016","journal-title":"Journal of Chromatography B"},{"key":"2020042702555697400_ref2","first-page":"1","article-title":"A review on medicinal uses, analytical techniques and pharmacological activites of Strychno nux-vomica Linn.: a concise report","author":"Patel","year":"2017","journal-title":"Chinese Journal of Integrative 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