{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T20:22:44Z","timestamp":1781554964117,"version":"3.54.5"},"reference-count":28,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2020,4,1]],"date-time":"2020-04-01T00:00:00Z","timestamp":1585699200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"Project of the Science Foundation for Distinguished Young Scholars of Guangdong Province","award":["2014A030306043"],"award-info":[{"award-number":["2014A030306043"]}]},{"name":"Major Project for International Cooperation and Exchange of the National Natural Science Foundation of China","award":["81220108028"],"award-info":[{"award-number":["81220108028"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2020,6,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Xian-Ling-Gu-Bao (XLGB), a famous traditional Chinese medicine prescription consisted of six herbal medicines, was used for prevention and treatment of osteoporosis in China. As an oral formulation, the multiple components contained in XLGB were inevitably biotransformed by the intestinal microflora before absorption via the gastrointestinal tract. However, the dynamic profiles of biotransformation products of XLGB remain unknown. In this paper, a rapid and sensitive ultra-performance liquid chromatography tandem triple quadrupole mass spectrometry method was developed for the simultaneous quantitative analysis of multiple biotransformation products of XLGB with rat intestinal microflora. For 10 selected quantitative compounds, all calibration curves revealed good linearity (r2\u00a0&amp;gt;\u00a00.99) within the sampling ranges considered. The whole intra- and inter-day precisions (as relative standard deviation) of all analytes were &amp;lt;13.5%, and the accuracies (as relative error) were in the range from \u221211.3 to 11.2%. The lower limits of quantification were 20, 10, 5, 20, 2, 2, 2, 5, 2 and 2\u00a0ng\/mL for sweroside, timosaponin BII, epimedin C, asperosaponin VI, psoralen, isobavachin, icariside II, timosaponin AIII, isobavachalcone and icaritin, respectively. The matrix effects, extraction recoveries and stabilities were all satisfactory. Meanwhile, dynamic profiles of 21 additional biotransformation products were also monitored by their area-time curves. The analytical method was successfully applied to describe dynamic profiles of 31 biotransformation products of XLGB and the recipes with removal of a definite composed herbal medicine (Anemarrhenae Rhizoma or Rehmanniae Radix).<\/jats:p>","DOI":"10.1093\/chromsci\/bmaa012","type":"journal-article","created":{"date-parts":[[2020,2,25]],"date-time":"2020-02-25T20:13:47Z","timestamp":1582661627000},"page":"494-503","source":"Crossref","is-referenced-by-count":7,"title":["Simultaneous Quantitative Analysis of Multiple Biotransformation Products of Xian-Ling-Gu-Bao, a Traditional Chinese Medicine Prescription, with Rat Intestinal Microflora by Ultra-Performance Liquid Chromatography Tandem Triple Quadrupole Mass Spectrometry"],"prefix":"10.1093","volume":"58","author":[{"given":"Xi-Yang","family":"Tang","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, West Huangpu Avenue No.601, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Meng-Xue","family":"Gao","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, West Huangpu Avenue No.601, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hui-Hui","family":"Xiao","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), The Hong Kong Polytechnic University Shenzhen Research Institute, Nanshan District, Shenzhen 518057, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Wei-Jing","family":"Yun","sequence":"first","affiliation":[{"name":"College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road No. 103, Shenhe District, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Yi","family":"Dai","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, West Huangpu Avenue No.601, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Zhi-Hong","family":"Yao","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, West Huangpu Avenue No.601, Guangzhou 510632, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Man-Sau","family":"Wong","sequence":"first","affiliation":[{"name":"State Key Laboratory of Chinese Medicine and Molecular Pharmacology (Incubation), The Hong Kong Polytechnic University Shenzhen Research Institute, Nanshan District, Shenzhen 518057, China"},{"name":"Department of Applied Biology and Chemical Technology, The Hong Kong Polytechnic University, Hung Hom, Kowloon, Hong Kong, 999077, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xin-Sheng","family":"Yao","sequence":"first","affiliation":[{"name":"Institute of Traditional Chinese Medicine and Natural Products, College of Pharmacy, Jinan University, West Huangpu Avenue No.601, Guangzhou 510632, China"},{"name":"College of Traditional Chinese Materia Medica, Shenyang Pharmaceutical University, Wenhua Road No. 103, Shenhe District, Shenyang 110016, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,4,1]]},"reference":[{"key":"2020060505300717000_ref1","volume-title":"National standards assembly of Chinese patent medicine; standard no. 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