{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,10,19]],"date-time":"2025-10-19T16:09:33Z","timestamp":1760890173777,"version":"3.37.3"},"reference-count":18,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T00:00:00Z","timestamp":1606694400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100004731","name":"Natural Science Foundation of Zhejiang Province","doi-asserted-by":"publisher","award":["LY15B060007"],"award-info":[{"award-number":["LY15B060007"]}],"id":[{"id":"10.13039\/501100004731","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81403117"],"award-info":[{"award-number":["81403117"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,2,15]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Five non-polar phloroglucinol derivatives, viz. pseudo-aspidin, \u03b1-kosin and agripinol A-C were isolated and purified from Agrimonia pilosa Ledeb by semi-preparative counter-current chromatography. The separation was performed by a two-step elution with non-aqueous solvent systems. In the first step, an elution mode of a two-phase solvent system consisting of n-hexane-acetonitrile-dichloromethane-methanol (6:6:0.5:0.5, v\/v\/v\/v) was used. We obtained sample \u0399 containing three components (47.0\u00a0mg) and sample \u0399\u0399 containing two components (24.8\u00a0mg) from crude extract (371.0\u00a0mg). In the second step, sample \u0399 was successfully separated by closed-loop recycling mode with a solvent system consisting of n-hexane-acetonitrile-dichloromethane (10:7:3, v\/v\/v), yielding 17.8\u00a0mg of pseudo-aspidin, 18.5\u00a0mg of \u03b1-kosin and 6.4\u00a0mg of agripinol A. The other two compounds\u20148.7\u00a0mg of agripinol B and 13.6\u00a0mg of agripinol C\u2014were obtained from sample \u0399\u0399 in the same manner. All the isolated compounds had a high purity exceeding 95%.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaa087","type":"journal-article","created":{"date-parts":[[2020,11,30]],"date-time":"2020-11-30T12:08:06Z","timestamp":1606738086000},"page":"212-216","source":"Crossref","is-referenced-by-count":2,"title":["Isolation and Purification of Five Phloroglucinol Derivatives From <i>Agrimonia Pilosa<\/i> Ledeb by Counter-Current Chromatography Using Non-Aqueous Solvent Systems"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0002-8667-5126","authenticated-orcid":false,"given":"Lulu","family":"Fu","sequence":"first","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang University of Technology, 288 Liuhe Rd, Hangzhou 310014, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chenghua","family":"Lu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang University of Technology, 288 Liuhe Rd, Hangzhou 310014, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Shengqiang","family":"Tong","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang University of Technology, 288 Liuhe Rd, Hangzhou 310014, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Min","family":"Xu","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang University of Technology, 288 Liuhe Rd, Hangzhou 310014, P.R. China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Lan","family":"Tang","sequence":"additional","affiliation":[{"name":"College of Pharmaceutical Science, Zhejiang University of Technology, 288 Liuhe Rd, Hangzhou 310014, P.R. 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