{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,6]],"date-time":"2026-10-06T01:41:47Z","timestamp":1791250907199,"version":"4.1.0"},"reference-count":21,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2020,11,21]],"date-time":"2020-11-21T00:00:00Z","timestamp":1605916800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Two chromatographic methods were validated for the determination of the widely prescribed analgesic and antipyretic drug combination of paracetamol (PC) (recently integrated into the supportive treatment of COVID-19), propyphenazone (PZ) and caffeine (CF) in the presence of two PC impurities, namely 4-aminophenol and 4-nitrophenol. A \u201cdual-mode\u201d gradient high-performance liquid chromatography method was developed, where the separation was achieved via \u201cdual-mode\u201d gradient by changing both the ternary mobile phase composition (acetonitrile: methanol: water) and the flow rate. This enables a good resolution within a relatively shorter analysis time. The analysis was realized using Zorbax Eclipse XDB column C18, 5\u00a0\u03bcm (250\u00a0\u00d7\u00a04.6\u00a0mm) and the UV detector was set at 220\u00a0nm. The other method is a thin-layer chromatography densitometry method, where the separation was achieved using a mobile phase composed of chloroform: toluene: ethyl acetate: methanol: acetic acid (6: 6: 1: 2: 0.1, by volume). Densitometric detection was performed at 220\u00a0nm on silica gel 60\u00a0F254 plates. The developed methods were fully validated as per the ICH guidelines and proved to be accurate, robust, specific and suitable for application as purity indicating methods for routine analysis of PC in pure form or in pharmaceuticals with PZ and CF in quality control laboratories.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaa088","type":"journal-article","created":{"date-parts":[[2020,10,20]],"date-time":"2020-10-20T07:18:59Z","timestamp":1603178339000},"page":"140-147","source":"Crossref","is-referenced-by-count":30,"title":["Simultaneous Determination of Paracetamol, Propyphenazone and Caffeine in Presence of Paracetamol Impurities Using Dual-Mode Gradient HPLC and TLC Densitometry Methods"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-7179-693X","authenticated-orcid":false,"given":"Hany","family":"Ibrahim","sequence":"first","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo-Suez road, Badr City 11829, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-8218-7512","authenticated-orcid":false,"given":"Abdallah M","family":"Hamdy","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy, Egyptian Russian University, Cairo-Suez road, Badr City 11829, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Hanan A","family":"Merey","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini street, 11562 Cairo, Egypt"},{"name":"Analytical Chemistry Department, Faculty of Pharmacy, October 6 University, 6th of October city, 12585 Giza, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed S","family":"Saad","sequence":"additional","affiliation":[{"name":"Analytical Chemistry Department, Faculty of Pharmacy, Cairo University, Kasr El-Aini street, 11562 Cairo, Egypt"},{"name":"Pharmaceutical Chemistry Department, School of Pharmacy and Pharmaceutical Industries, Badr University in Cairo (BUC), Badr City, 11829 Cairo, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2020,11,21]]},"reference":[{"key":"2021011501244130500_ref1","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1016\/j.jchromb.2007.07.008","article-title":"Determination of non-steroidal anti-inflammatory drugs in pharmaceuticals and human serum by dual-mode gradient HPLC and fluorescence detection","volume":"857","author":"Hany","year":"2007","journal-title":"Journal of Chromatography. 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