{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,22]],"date-time":"2026-03-22T18:24:34Z","timestamp":1774203874792,"version":"3.50.1"},"reference-count":11,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,1,5]],"date-time":"2021-01-05T00:00:00Z","timestamp":1609804800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A novel, delicate, stability-indicating, gradient, reversed-phase high-performance liquid chromatographic method has been established for the quantitative estimation of methocarbamol (MTC) and its impurities present in a pharmaceutical oral suspension. XBridge C18, 5\u2009\u03bcm, 250\u00a0mm\u2009\u00d7\u20094.6\u2009mm column was used to accomplish chromatographic separation with a buffered mobile phase consisting of a mixture of 0.01\u2009M of sodium dihydrogen phosphate (pH\u00a07.0 buffer) and methanol in the ratio of 95:05 (v\/v), respectively, were used as solvent A and a mixture of methanol and Milli-Q water in the ratio 90:10 (v\/v), respectively, was used as solvent B. Analysis was carried out at 0.8\u2009mL\/min flow rate and the detection wavelength at 225\u2009nm. The compartment temperature of the column is put at 25\u00b0C. The resolution of MTC and its four impurities has been attained &amp;gt;2.0 for all pairs of compounds. Significant degradation of MTC was photolytic, thermal and oxidative stress conditions. Validation of the developed method was performed as stated by the International Conference on Harmonization guidelines with regard to all validation parameters like specificity, accuracy, linearity, precision, limit of detection, limit of quantitation and robustness.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaa125","type":"journal-article","created":{"date-parts":[[2020,12,1]],"date-time":"2020-12-01T20:38:05Z","timestamp":1606855085000},"page":"555-565","source":"Crossref","is-referenced-by-count":5,"title":["Development and Validation of a Stability-Indicating High-Performance Liquid Chromatographic Method for the Quantification of Methocarbamol and Its Impurities in Pharmaceutical Dosage Forms"],"prefix":"10.1093","volume":"59","author":[{"given":"Shivani","family":"Kalokhe","sequence":"first","affiliation":[{"name":"Modern College of Pharmacy, Nigdi, QAT, Pune, Maharashtra 412109, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Santaji","family":"Nalwade","sequence":"additional","affiliation":[{"name":"Callidus Research Laboratories Pvt. Ltd, PAP-A-29\/1, Chakan Industrial Area Phase-IV, Nighoje, Tal-Khed, Pune, Maharashtra 410501, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2089-0288","authenticated-orcid":false,"given":"Pallavi","family":"Patil","sequence":"additional","affiliation":[{"name":"Modern College of Pharmacy, Nigdi, QAT, Pune, Maharashtra 412109, India"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Poonam","family":"Raskar","sequence":"additional","affiliation":[{"name":"Modern College of Pharmacy, Nigdi, QAT, Pune, Maharashtra 412109, India"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,1,5]]},"reference":[{"issue":"1","key":"2021052106393418900_ref1","first-page":"258","article-title":"Method development and validation of methocarbamol in bulk and its formulation by UV spectroscopy","volume":"5","author":"Nagamani","year":"2015","journal-title":"International Journal of Pharmaceutical, Chemical and Biological Sciences"},{"issue":"1","key":"2021052106393418900_ref2","first-page":"169","article-title":"Method development, validation and degradation studies of methocarbamol from methocarbamol injection","volume":"50","author":"Prabha","year":"2018","journal-title":"International Journal of Pharmaceutical Sciences Review and Research"},{"key":"2021052106393418900_ref3"},{"issue":"8","key":"2021052106393418900_ref4","doi-asserted-by":"crossref","first-page":"231","DOI":"10.7897\/2230-8407.04823","article-title":"Simple UV spectrometric method for estimation of methocarbamol in bulk and its formulation","volume":"4","author":"Meher","year":"2013","journal-title":"International Research Journal of Pharmacy"},{"issue":"3","key":"2021052106393418900_ref5","first-page":"112","article-title":"A validated stability indicating HPLC assay method for methocarbamol in bulk formulation","volume":"1","author":"Priyanka","year":"2014","journal-title":"Indian Journal of Medical Research"},{"issue":"4","key":"2021052106393418900_ref6","doi-asserted-by":"crossref","first-page":"1620","DOI":"10.1155\/2011\/614748","article-title":"Simultaneous HPLC determination of methocarbamol, paracetamol and diclofenac sodium","volume":"8","author":"Hafza","year":"2012","journal-title":"E-Journal of Chemistry"},{"issue":"4","key":"2021052106393418900_ref7","first-page":"1833","article-title":"Development and validation of RP-HPLC method for simultaneous determination of methocarbamol and diclofenac sodium in injection dosage form","volume":"5","author":"Patel","year":"2016","journal-title":"World Journal of Plastic and Reconstructive Surgery"},{"key":"2021052106393418900_ref8","first-page":"8714","author":"United State Pharmacopeia","year":"2016"},{"key":"2021052106393418900_ref9","volume-title":"International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use","author":"ICH Q1A (R2)","year":"2003"},{"key":"2021052106393418900_ref10","volume-title":"International Conference on Harmonisation of Technical Requirements for Registration of Pharmaceuticals for Human Use","author":"ICH, Q2 (R1)","year":"1996"},{"key":"2021052106393418900_ref11","doi-asserted-by":"crossref","first-page":"21","DOI":"10.4103\/2229-5186.79345","article-title":"Methods for the determination of limit of detection and limit of quantitation of the analytical methods","volume":"2","author":"Shrivastava","year":"2011","journal-title":"Chronicles of Young Scientists"}],"container-title":["Journal of Chromatographic Science"],"original-title":[],"language":"en","link":[{"URL":"http:\/\/academic.oup.com\/chromsci\/article-pdf\/59\/6\/555\/37975676\/bmaa125.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"http:\/\/academic.oup.com\/chromsci\/article-pdf\/59\/6\/555\/37975676\/bmaa125.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2021,5,21]],"date-time":"2021-05-21T06:40:13Z","timestamp":1621579213000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/chromsci\/article\/59\/6\/555\/6062653"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2021,1,5]]},"references-count":11,"journal-issue":{"issue":"6","published-online":{"date-parts":[[2021,1,5]]},"published-print":{"date-parts":[[2021,5,20]]}},"URL":"https:\/\/doi.org\/10.1093\/chromsci\/bmaa125","relation":{},"ISSN":["0021-9665","1945-239X"],"issn-type":[{"value":"0021-9665","type":"print"},{"value":"1945-239X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2021,7]]},"published":{"date-parts":[[2021,1,5]]}}}