{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:23:42Z","timestamp":1740165822975,"version":"3.37.3"},"reference-count":17,"publisher":"Oxford University Press (OUP)","issue":"6","license":[{"start":{"date-parts":[[2021,3,29]],"date-time":"2021-03-29T00:00:00Z","timestamp":1616976000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2021,5,20]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A rapid, simple and sensitive liquid chromatography tandem mass spectrometry method for the determination of methohexital in human whole blood was developed and validated. Ethyl acetate\/n-hexane (9:1) was used as extraction solvent while aprobarbital was used as internal standard. Methohexital was recovered by liquid\u2013liquid extraction from 100\u00a0\u03bcL of human whole blood. The mobile phase was water-acetonitrile, and an ACQUITY BEH C18 (2.1\u00a0\u00d7\u00a0100\u00a0mm, 1.7\u00a0\u03bcm) column was adopted. Negative electrospray ionization source and multiply reaction monitoring mode were applied. The transitions of m\/z were 261.2\/42.2 and 261.2\/119.0 for methohexital. The limit of detection was 0.5\u00a0ng\/mL, which was lower than the previous methods. Wide linear range (2\u20132,000\u00a0ng\/mL) with a good correlation coefficient (r\u00a0&amp;gt;\u00a00.99) was also obtained. The intra- and inter- day precisions represented by relative standard deviation were &amp;lt;11.5%, and the recoveries were &amp;gt;79.67%. This analytical method involved small sample volume and had been proven to be rapid, easy, sensitive and specific. Therefore, it could be used for the clinical analysis of methohexital.<\/jats:p>","DOI":"10.1093\/chromsci\/bmab024","type":"journal-article","created":{"date-parts":[[2021,3,27]],"date-time":"2021-03-27T12:09:19Z","timestamp":1616846959000},"page":"521-525","source":"Crossref","is-referenced-by-count":0,"title":["Quantitative Determination of Methohexital in Human Whole Blood by Liquid Chromatography Tandem Mass Spectrometry"],"prefix":"10.1093","volume":"59","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1377-5748","authenticated-orcid":false,"given":"Yi","family":"Zhang","sequence":"first","affiliation":[{"name":"School of Chemical Engineering, Nanjing University of Science and Technology, Xiaolingwei Road 200, Nanjing, Jiangsu 210094, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Yunfeng","family":"Zhang","sequence":"additional","affiliation":[{"name":"Institute of Forensic Science, Ministry of Public Security, Muxidi South Lane No. 17, Beijing 100038, China"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Chunfeng","family":"Zhai","sequence":"additional","affiliation":[{"name":"Qilu Pharmaceutical Co., Ltd, Lvyou Road 8888, Jinan, Shandong 250104, China"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,3,29]]},"reference":[{"author":"Brevital Sodium","key":"2021070819424876800_ref1"},{"issue":"6","key":"2021070819424876800_ref2","doi-asserted-by":"crossref","first-page":"665","DOI":"10.1001\/archpedi.155.6.665","article-title":"Use of intravenous methohexital as a sedative in pediatric emergency departments","volume":"155","author":"Sedik","year":"2001","journal-title":"Archives of Pediatrics and Adolescent Medicine"},{"volume-title":"Quantitative analysis of barbiturates in urine using 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