{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,20]],"date-time":"2026-08-20T15:19:30Z","timestamp":1787239170171,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2021,6,30]],"date-time":"2021-06-30T00:00:00Z","timestamp":1625011200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["32002050"],"award-info":[{"award-number":["32002050"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100012166","name":"National Key Research and Development Program of China","doi-asserted-by":"publisher","award":["2018YFD0200804"],"award-info":[{"award-number":["2018YFD0200804"]}],"id":[{"id":"10.13039\/501100012166","id-type":"DOI","asserted-by":"publisher"}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["Y2019PT17-02"],"award-info":[{"award-number":["Y2019PT17-02"]}]},{"name":"Central Public-interest Scientific Institution Basal Research Fund","award":["Y2020PT29"],"award-info":[{"award-number":["Y2020PT29"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,3,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>The volatile compound 6-methyl-5-hepten-2-one (MHO) is an important chemical intermediate and flavor component derived from carotenoid during fruit metabolism, which is important factor impacting consumer-liking. Fruit has complex matrix and is difficult to be purified during MHO analysis. A rapid liquid\u2013liquid extraction-gas chromatography\u2013mass spectrometry (LLE-GC\u2013MS) method was developed for effective determination of MHO in fruit in this study. The calibration curves for MHO showed high linearity in the range of 100\u20132000\u00a0ng\/ml with internal standard method. The limit of detection (LOD), limit of quantification (LOQ) were both &amp;lt;100\u00a0ng\/ml. Real samples of tomato cultivar fruit were analyzed and RSD of biological replicates varied in the range of 2.01% to 12.59%. MHO showed varied content in different tomato fruit tissues of the selected cultivars, in addition to the variation between cultivars. Furthermore, the possible fragmentation patterns were proposed for the main characteristic ions of MHO and internal standard, based on isolation of the fragment ions after the precursor ion underwent splitting at the electron impact ion source. Compared with reported solid-phase microextraction (SPME)-GC\u2013MS method, the validated method was more rapid, precise, accurate and low cost for MHO quantitation.<\/jats:p>","DOI":"10.1093\/chromsci\/bmab071","type":"journal-article","created":{"date-parts":[[2021,5,28]],"date-time":"2021-05-28T15:16:46Z","timestamp":1622215006000},"page":"280-286","source":"Crossref","is-referenced-by-count":17,"title":["Rapid Determination of 6-Methyl-5-hepten-2-one in Fruit with LLE-GC-MS"],"prefix":"10.1093","volume":"60","author":[{"given":"Zhen","family":"Zhou","sequence":"first","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun Nandajie, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xiuqing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun Nandajie, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Xia","family":"Cui","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun Nandajie, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7417-0876","authenticated-orcid":false,"given":"Haijing","family":"Wang","sequence":"additional","affiliation":[{"name":"Key Laboratory of Biology and Genetic Improvement of Horticultural Crops of the Ministry of Agriculture, Sino-Dutch Joint Laboratory of Horticultural Genomics, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, No. 12 Zhongguancun Nandajie, Haidian District, Beijing 100081, China"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,6,30]]},"reference":[{"issue":"7","key":"2022032516092204300_ref1","doi-asserted-by":"crossref","first-page":"964","DOI":"10.1080\/01904167.2016.1231812","article-title":"Abscisic acid improves tomato fruit quality by increasing soluble sugar concentrations","volume":"40","author":"Barickman","year":"2017","journal-title":"Journal of Plant Nutrition"},{"issue":"August","key":"2022032516092204300_ref2","first-page":"1286","article-title":"Association mapping of main tomato fruit sugars and organic acids","volume":"7","author":"Zhao","year":"2016","journal-title":"Frontiers in Plant Science"},{"issue":"6","key":"2022032516092204300_ref3","doi-asserted-by":"crossref","first-page":"1451","DOI":"10.1093\/jxb\/ert035","article-title":"What controls fleshy fruit acidity? 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