{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,2,21]],"date-time":"2025-02-21T19:23:47Z","timestamp":1740165827470,"version":"3.37.3"},"reference-count":43,"publisher":"Oxford University Press (OUP)","issue":"7","license":[{"start":{"date-parts":[[2021,9,20]],"date-time":"2021-09-20T00:00:00Z","timestamp":1632096000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,9,3]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Two sensitive, selective and precise chromatographic methods have been established for concomitant quantification of ciprofloxacin HCl (CIP), fluocinolone acetonide (FLU) along with ciprofloxacin impurity A (CIP-imp A). The first method was thin-layer chromatography (TLC-densitometry) where separation was accomplished using TLC silica plates 60 G.F254 as a stationary phase and chloroform\u2013methanol\u201333%ammonia (4.6:4.4:1, by volume) as a developing system. The obtained plates were scanned at 260\u00a0nm over concentration ranges of 1.0\u201340.0, 0.6\u201320.0 and 1.0\u201340.0\u00a0\u03bcg band\u22121 for CIP, FLU and CIP-imp A, respectively. The second method was based on high-performance liquid chromatography using a Zorbax ODS column (5\u2009\u03bcm, 150\u2009\u00d7\u20094.6\u00a0mm i.d.) where adequate separation was achieved through a mobile phase composed of phosphate buffer pH\u00a03.6\u2013acetonitrile (45:55, v\/v) at flow rate 1.0\u00a0mL\u00a0min\u22121 with ultraviolet detection at 254\u00a0nm. Linear regressions were obtained in the range of 1.0\u201340.0\u00a0\u03bcg\u00a0mL\u22121 for CIP, 0.6\u201320.0\u00a0\u03bcg\u00a0mL\u22121 for FLU and 1.0\u201340.0\u00a0\u03bcg\u00a0mL\u22121 for CIP-imp A. The suggested methods were validated in compliance with the International Conference on Harmonization guidelines and were successfully applied for determination of CIP and FLU in bulk powder and newly marketed otic solution.<\/jats:p>","DOI":"10.1093\/chromsci\/bmab110","type":"journal-article","created":{"date-parts":[[2021,9,8]],"date-time":"2021-09-08T11:19:16Z","timestamp":1631099956000},"page":"655-662","source":"Crossref","is-referenced-by-count":6,"title":["Two Validated Chromatographic Methods for Determination of Ciprofloxacin HCl, One of its Specified Impurities and Fluocinolone Acetonide in Newly Approved Otic Solution"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3756-8065","authenticated-orcid":false,"given":"Mahmoud A","family":"Tantawy","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy , Analytical Chemistry Department, , Kasr el Aini Street, Cairo, 11562 , Egypt"},{"name":"Cairo University , Analytical Chemistry Department, , Kasr el Aini Street, Cairo, 11562 , Egypt"},{"name":"Faculty of Pharmacy , Chemistry Department, , 6 October City, Giza, 12585 , Egypt"},{"name":"October 6 University , Chemistry Department, , 6 October City, Giza, 12585 , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9169-1454","authenticated-orcid":false,"given":"Israa A","family":"Wahba","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , , 6th of October City, Giza, 12568 , Egypt"},{"name":"College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science & Technology , , 6th of October City, Giza, 12568 , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Samah S","family":"Saad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , , 6th of October City, Giza, 12568 , Egypt"},{"name":"College of Pharmaceutical Sciences and Drug Manufacturing, Misr University for Science & Technology , , 6th of October City, Giza, 12568 , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nesrin K","family":"Ramadan","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy , Analytical Chemistry Department, , Kasr el Aini Street, Cairo, 11562 , Egypt"},{"name":"Cairo University , Analytical Chemistry Department, , Kasr el Aini Street, Cairo, 11562 , Egypt"}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2021,9,20]]},"reference":[{"volume-title":"Clarke\u2019s analysis of drugs and poisons","year":"2011","author":"Moffat","key":"2022090302181286800_ref1"},{"issue":"4","key":"2022090302181286800_ref2","doi-asserted-by":"crossref","first-page":"307","DOI":"10.1016\/j.ijantimicag.2007.12.010","article-title":"Quinolone resistance in the food chain","volume":"31","author":"F\u00e0brega","year":"2008","journal-title":"International Journal of 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