{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,1]],"date-time":"2026-08-01T07:34:10Z","timestamp":1785569650597,"version":"3.56.0"},"reference-count":39,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2021,12,8]],"date-time":"2021-12-08T00:00:00Z","timestamp":1638921600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,11,5]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>From evolution, thin-layer chromatography (TLC) attracts attention as a versatile technique for efficient separation and identification of many drug substances and chemicals. Owing to its simplicity and other outstanding advantages, TLC is extensively used by chromatographers in quantification and purity profiling objectives. In the present study two TLC-Densitometric methods are established and validated for the synchronous estimation of Cinnarizine (Cinn) and Acefyline Heptaminol (Acef) in the presence of Cinn\/Acef reported degradation products and Thoephylline (Theo) as Acef potential impurity. The proposed methods are based on densitometric measurements of the spots of Cinn and Acef after separation from their degradation products. Separation is attained on silica gel sheet with dichloromethane: methanol: formic acid as a developing system in ratio: (15, 1, 0.5, by volume) and (15, 0.75, 0.4, by volume) for Cinn (method 1) and Acef (method 2) degradation, consecutively. Quantification is done at 254\u00a0nm over concentration ranges of 0.2\u20131.8 and 2\u201318\u00a0\u03bcg\/spot for Cinn and Acef; respectively, with mean percentage recoveries of 99.18\u2009\u00b1\u20090.60\/99.84\u2009\u00b1\u20090.53 and 99.19\u2009\u00b1\u20090.93\/99.66\u2009\u00b1\u20090.58 for method 1 and method 2; consecutively. The two methods are fully validated and proven to be selective, robust and retained their accuracy in up to 50% of Cinn\/Acef reported degradation products and Theo. Moreover, the two methods are applied to a coformulated drug product comprising Cinn and Acef showing satisfactory results. Comparison of the obtained results by the proposed methods with that of the reference ones statistically shows no significant differences.<\/jats:p>","DOI":"10.1093\/chromsci\/bmab129","type":"journal-article","created":{"date-parts":[[2021,11,2]],"date-time":"2021-11-02T12:36:47Z","timestamp":1635856607000},"page":"832-839","source":"Crossref","is-referenced-by-count":2,"title":["Versatile TLC-Densitometric Methods for the Synchronous Estimation of Cinnarizine and Acefylline Heptaminol in The Presence of Potential Impurity and Their Reported Degradation Products"],"prefix":"10.1093","volume":"60","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1779-5412","authenticated-orcid":false,"given":"Ola M","family":"El-Houssini","sequence":"first","affiliation":[{"name":"National Organization for Drug Control and Research (NODCAR) , 51 wezerat El- Zeraa Street St, Agouza P.O. Box 12553 Giza 35521, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Mohammad A","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562, Egypt"},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562, Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2021,12,8]]},"reference":[{"key":"2022110707380753800_ref1","doi-asserted-by":"crossref","first-page":"43","DOI":"10.1016\/S0021-9673(01)97364-9","article-title":"Thin layer chromatography of dyestuffs on polyamide and \u201csilver nitrate\u201d layers","volume":"20","author":"Copius-Peereboom","year":"1965","journal-title":"Journal of Chromatography"},{"key":"2022110707380753800_ref2","doi-asserted-by":"crossref","first-page":"799","DOI":"10.1093\/chromsci\/bmz055","article-title":"HPTLC method for determination of R, 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