{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,2]],"date-time":"2026-10-02T17:30:34Z","timestamp":1790962234723,"version":"4.1.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2022,1,13]],"date-time":"2022-01-13T00:00:00Z","timestamp":1642032000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"IARSCS"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2022,6,6]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A gas chromatographic (GC) procedure has been developed for the determination of fluorouracil (5-FU) after pre-column derivatization with hexafluoroacetylacetone. GC separation was from column DB-1 (30\u00a0m\u00a0\u00d7\u00a00.32\u00a0mm id) and the determination was by flame-ionization detection. The derivatization conditions were optimized at pH\u00a04, heating at 90\u00b0C for 40\u00a0min and extraction of the derivative was in chloroform. Using the conditions nucleobases cytosine, uracil, thymine, adenine and guanine separated completely from fluorouracil. The linear calibration range and LOD for 5-fluorouracil were 0.5\u201340.0 and 0.2\u00a0\u03bcg\/mL, respectively. The derivatization, elution and separation were repeatable in terms of retention time and peak height\/peak area (n\u2009=\u20095) and relative standard deviations (RSD) were within 3.5%. The method was applied for the analysis of serum spiked with 5-FU with recovery of 95.5\u201397.5% with RSD 1.5\u20133.1%.<\/jats:p>","DOI":"10.1093\/chromsci\/bmab142","type":"journal-article","created":{"date-parts":[[2021,12,29]],"date-time":"2021-12-29T13:01:56Z","timestamp":1640782916000},"page":"409-413","source":"Crossref","is-referenced-by-count":4,"title":["GC Determination of Fluorouracil in Serum by Using Hexafluroroacetylacetone as Derivatizing Reagent"],"prefix":"10.1093","volume":"60","author":[{"given":"Rizwan Ali","family":"Zounr","sequence":"first","affiliation":[{"name":"Institute of Advanced Research Studies in Chemical Sciences, University of Sindh , Jamshoro-76080, Sindh, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Muhammad Yar","family":"Khuhawar","sequence":"additional","affiliation":[{"name":"Institute of Advanced Research Studies in Chemical Sciences, University of Sindh , Jamshoro-76080, Sindh, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Taj Muhammad Jahangir","family":"Khuhawar","sequence":"additional","affiliation":[{"name":"Institute of Advanced Research Studies in Chemical Sciences, University of Sindh , Jamshoro-76080, Sindh, Pakistan"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-8794-7296","authenticated-orcid":false,"given":"Muhammad Farooque","family":"Lanjwani","sequence":"additional","affiliation":[{"name":"Dr M. 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