{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,23]],"date-time":"2026-10-23T20:04:01Z","timestamp":1792785841972,"version":"4.5.0"},"reference-count":14,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2022,12,6]],"date-time":"2022-12-06T00:00:00Z","timestamp":1670284800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/journals\/pages\/open_access\/funder_policies\/chorus\/standard_publication_model"}],"funder":[{"name":"PAION GmhH"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,3,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A new assay was developed to measure the concentration of remimazolam besylate (CNS7056B) and its major carboxylic acid metabolite (CNS7054X) in human plasma. For this new assay method, midazolam-d4 maleate was used as an internal standard. After setting up a previously described assay method, using CNS7056-d4 and CNS7054-d4 as internal standards, analytical results of both methods were compared. For the new analytical method, ultra-high-performance liquid chromatography (UHPLC) with tandem mass spectrometry was applied. A purification method, using solid phase extraction, was developed and validated. The chromatographic separation of the analytes was achieved with a mobile phase gradient using a Water Acquity\u2122 UHPLC-System. The Kinetex\u2122 biphenyl 50 \u00d7 2.1\u00a0mm UHPLC column was used with a particle diameter of 1.7\u00a0\u03bcm (Phenomenex, Germany). A measuring range of 0.6\u20132,000\u00a0ng\/mL for CNS7056B and of 6\u201320,000\u00a0ng\/mL for CNS7054X could be achieved with this new assay. The lower limit of quantification was 0.6\u00a0ng\/mL for CNS7056B and 6\u00a0ng\/mL for CNS7054X. The assay was validated according to US Food and Drug Administration guidelines. The new method showed an accuracy of 96.9\u2013110.4% and a precision of 2.1\u20136.7% for both analytes.<\/jats:p>","DOI":"10.1093\/chromsci\/bmac097","type":"journal-article","created":{"date-parts":[[2022,11,3]],"date-time":"2022-11-03T17:43:02Z","timestamp":1667497382000},"page":"232-240","source":"Crossref","is-referenced-by-count":2,"title":["Quantification of Remimazolam Besylate (CNS7056B) and Its Metabolite (CNS7054X) by LC\u2013MS\/MS in Human Plasma Using Midazolam-d4 Maleate as Internal Standard"],"prefix":"10.1093","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-1985-6733","authenticated-orcid":false,"given":"Stefanie","family":"Schmidt","sequence":"first","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ljubica","family":"Krajinovic","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Christian","family":"Mertens","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Lisa","family":"Schramm","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00fcrgen","family":"Sch\u00fcttler","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"J\u00f6rg","family":"Fechner","sequence":"additional","affiliation":[{"name":"Department of Anesthesiology, Friedrich-Alexander-University of Erlangen-Nuremberg , Krankenhausstra\u00dfe 12, 91054 Erlangen , Germany"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2022,12,6]]},"reference":[{"issue":"4","key":"2024032511260123700_ref1","doi-asserted-by":"crossref","first-page":"636","DOI":"10.1097\/ALN.0000000000003103","article-title":"Pharmacokinetics and pharmacodynamics of remimazolam (CNS 7056) after continuous infusion in healthy male volunteers: part I. pharmacokinetics and clinical pharmacodynamics","volume":"132","author":"Sch\u00fcttler","year":"2020","journal-title":"Anesthesiology"},{"issue":"2","key":"2024032511260123700_ref2","doi-asserted-by":"crossref","first-page":"284","DOI":"10.1213\/ANE.0b013e318241f68a","article-title":"A placebo- and midazolam-controlled phase I single ascending-dose study evaluating the safety, pharmacokinetics, and pharmacodynamics of remimazolam (CNS 7056): part II. 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