{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,17]],"date-time":"2026-03-17T00:33:05Z","timestamp":1773707585760,"version":"3.50.1"},"reference-count":20,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2023,10,20]],"date-time":"2023-10-20T00:00:00Z","timestamp":1697760000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,11,1]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Nortriptyline HCl and pregabalin Tablet is used to treat neuropathic pain as well as mental or mood issues such as sadness, mood, feelings, anxiety and tensions. Very few analytical methods are available for the simultaneous estimation of nortriptyline HCl and pregabalin and no reports has been found for HPTLC method. In the current study, a reliable HPTLC method for the simultaneous measurement of nortriptyline HCl and pregabalin in pure forms and pharmaceutical formulations has been developed with ninhydrine post derivatization of pregabalin. The HPTLC method development was carried using silica gel G60 F254 as stationary phase and acetonitrile: methanol: triethylamine: water: formic acid (7:3:0.3:0.8:0.02\u00a0v\/v\/v\/v\/v) was used as mobile phase with saturation time of 20\u00a0min. The system was found to give a compact band for nortriptyline HCl (Rf\u2009=\u20090.523\u2009\u00b1\u20090.008) pregabalin (Rf\u2009=\u20090.279\u2009\u00b1\u20090.005). The developed method was found to be validated as per ICH Q2 (R1) guideline. The peak of nortriptyline HCl and pregabalin showed good linearity over the concentration range of 50\u2013300\u00a0ng\/band and 350\u20132250\u00a0ng\/band, respectively, with a correlation coefficient of \u02c30.995. The % recoveries of both drugs were found to be in the range of 98.84\u2013101.87%. Statistical analysis proved that the method is selective, precise, robust and accurate for the estimation of nortriptyline HCl and pregabalin.<\/jats:p>","DOI":"10.1093\/chromsci\/bmad081","type":"journal-article","created":{"date-parts":[[2023,10,21]],"date-time":"2023-10-21T02:44:21Z","timestamp":1697856261000},"page":"886-891","source":"Crossref","is-referenced-by-count":6,"title":["HPTLC Method Development and Validation for the Simultaneous Estimation of Nortriptyline HCl and Pregabalin in their Combined Dosage Form"],"prefix":"10.1093","volume":"62","author":[{"given":"Hinal","family":"Harkhani","sequence":"first","affiliation":[{"name":"Gujarat Technological University Graduate School of Pharmacy, , Ahmedabad, Gujarat ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Kashyap","family":"Thummar","sequence":"additional","affiliation":[{"name":"Gujarat Technological University Graduate School of Pharmacy, , Ahmedabad, Gujarat ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Sanjay","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Gujarat Technological University Graduate School of Pharmacy, , Ahmedabad, Gujarat ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jigna","family":"Vadalia","sequence":"additional","affiliation":[{"name":"Gujarat Technological University Graduate School of Pharmacy, , Ahmedabad, Gujarat ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2023,10,20]]},"reference":[{"key":"2024110205463230400_ref1","doi-asserted-by":"crossref","first-page":"1467","DOI":"10.2147\/JPR.S138099","article-title":"Combination treatment of neuropathic pain: Danish expert recommendations based on a Delphi process","volume":"10","author":"Holbech","year":"2017","journal-title":"Journal of Pain Research"},{"key":"2024110205463230400_ref2","author":"Indian Pharmacopoeia","year":"2022"},{"key":"2024110205463230400_ref3","doi-asserted-by":"crossref","first-page":"7","DOI":"10.1016\/j.jad.2012.06.007","article-title":"Selective serotonin reuptake inhibitors and tricyclic antidepressants in the acute treatment of chronic depression and dysthymia: A systematic review and meta-analysis","volume":"144","author":"Wolff","year":"2013","journal-title":"Journal of Affective Disorders"},{"key":"2024110205463230400_ref4","doi-asserted-by":"crossref","first-page":"7","DOI":"10.11607\/ofph.1886","article-title":"Nortriptyline compared to amitriptyline for the treatment of persistent masticatory myofascial pain","volume":"33","author":"Haviv","year":"2019","journal-title":"Journal of Oral & Facial Pain and Headache"},{"issue":"2","key":"2024110205463230400_ref5","doi-asserted-by":"crossref","first-page":"137","DOI":"10.1016\/j.eplepsyres.2006.09.008","article-title":"Pharmacology and mechanism of action of pregabalin: The calcium channel \u03b12\u2013\u03b4 (alpha2\u2013delta) subunit as a target for antiepileptic drug discovery","volume":"73","author":"Taylor","year":"2007","journal-title":"Epilepsy Research"},{"key":"2024110205463230400_ref6","author":"British Pharmacopoeia","year":"2019"},{"key":"2024110205463230400_ref7","author":"United States Pharmacopeial Convention","year":"2019"},{"issue":"3","key":"2024110205463230400_ref8","first-page":"464","article-title":"Mochi spectrophotometric method for simultaneous estimation of Nortriptylin hydrochloride and Pregabalin in tablet dosage","volume":"8","author":"Patel","year":"2020","journal-title":"Aegaeum Journal"},{"issue":"3","key":"2024110205463230400_ref9","first-page":"1","article-title":"A novel spectrophotometric and RP-HPLC methods for determination of nortriptyline hydrochloride and Pregabalin in tablets","volume":"9","author":"Patel","year":"2020","journal-title":"International Journal of Pharmaceutical 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Pregabalin in capsules","volume":"5","author":"Walash","year":"2011","journal-title":"Chemistry Central Journal"},{"key":"2024110205463230400_ref13","doi-asserted-by":"crossref","first-page":"59","DOI":"10.1186\/1752-153X-5-59","article-title":"A novel method for spectrophotometric determination of pregabalin in pure form and in capsules","volume":"5","author":"Bali","year":"2011","journal-title":"Chemistry Central Journal"},{"key":"2024110205463230400_ref14","first-page":"31","article-title":"Spectrophotometric method for pregabalin determination: An experimental design approach for method development","volume":"21","author":"Patil","year":"2016","journal-title":"Arab Journal of Basic and Applied Sciences"},{"issue":"4","key":"2024110205463230400_ref15","doi-asserted-by":"crossref","first-page":"421","DOI":"10.59566\/IJBS.2009.5421","article-title":"Shanker a sensitive spectrophotometric method for the determination of pregabalin in bulk, pharmaceutical formulations and in human urine 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