{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:47:32Z","timestamp":1753876052673,"version":"3.41.2"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,2,26]],"date-time":"2024-02-26T00:00:00Z","timestamp":1708905600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Cyproheptadine (CYP) is an antihistaminic and anti-serotonin drug used to improve the clinical course of hospitalized patients severely affected by COVID-19 and requiring supplemental oxygen. Thin layer chromatography (TLC)-densitometry method was developed and validated for the estimation of CYP and its impurity, dibenzosuberone and its degradation product: 10, 11-dihydroxy-dibenzosuberone. The toxicity profiles of CYP impurity and degradation product were studied and the in silico data, using pre-ADMET software, proved their toxicity. A developing system of hexane: methanol: ethyl acetate: formic acid: triethylamine (8:1:1:0.05:0.1, by volume) was used with densitometric scanning at 245\u00a0nm. International Conference of Harmonization guidelines were obeyed for method validation. The proposed method was applied on CYP pharmaceutical formulation whose results showed no significant difference from results of the reported chromatographic method regarding accuracy and precision. Method\u2019s greenness was evaluated by analytical Eco scale, Green Analytical Procedure Index, and Analytical greenness approach tools. The developed method can be applied for safety check analysis of CYP containing formulations and detection and even quantification of its related toxic components in quality control laboratories at high sensitivity up to 20-fold than the reported chromatographic method.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae004","type":"journal-article","created":{"date-parts":[[2024,2,13]],"date-time":"2024-02-13T13:21:10Z","timestamp":1707830470000},"source":"Crossref","is-referenced-by-count":0,"title":["Green Chromatographic Assay of Toxic Impurities of Cyproheptadine: In Silico Toxicity Profiling"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0001-6526-4419","authenticated-orcid":false,"given":"Raghda A","family":"Emam","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Beni-Suef University Pharmaceutical Analytical Chemistry Department, , Al shaheed Shehata Ahmed Hegazy street, 62514 Beni-Suef,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-4960","authenticated-orcid":false,"given":"Aml A","family":"Emam","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Beni-Suef University Pharmaceutical Analytical Chemistry Department, , Al shaheed Shehata Ahmed Hegazy street, 62514 Beni-Suef,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,2,26]]},"reference":[{"key":"2025010912420404400_ref1","volume-title":"The United States Pharmacopeia","author":"United States Pharmacopeia Convention INC","year":"2011","edition":"34th"},{"key":"2025010912420404400_ref2","volume-title":"Martindale: The Complete Drug References: The Extra Pharmacopoeia","author":"Sweetman","year":"2009","edition":"36th"},{"author":"Cyproheptadine in Severe COVID-19","key":"2025010912420404400_ref3"},{"volume-title":"Her Majesty\u2019s","year":"2013","author":"The British Pharmacopoeia","key":"2025010912420404400_ref4"},{"key":"2025010912420404400_ref5"},{"key":"2025010912420404400_ref6","doi-asserted-by":"crossref","first-page":"128","DOI":"10.1093\/chromsci\/bmaa085","article-title":"Development and validation of stability indicating high-performance liquid chromatographic method for determination of cyproheptadine hydrochloride, its impurity and degradation product","volume":"59","author":"Abdelrahman","year":"2021","journal-title":"Journal of Chromatographic Science"},{"key":"2025010912420404400_ref7","first-page":"360","article-title":"Application of bromate-bromide mixture and methyl orange in the titrimetric, spectrophotometric and kinetic assay methods for cyproheptadine in pharmaceuticals","volume":"13","author":"Basavaiah","year":"2006","journal-title":"Indian Journal of Chemical Technology"},{"key":"2025010912420404400_ref8","first-page":"444","article-title":"Determination of cyproheptadine 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