{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,2]],"date-time":"2026-06-02T23:36:38Z","timestamp":1780443398653,"version":"3.54.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2024,3,28]],"date-time":"2024-03-28T00:00:00Z","timestamp":1711584000000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2024,5,31]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This technique is employed for the simultaneous quantification of aspirin (ASP) and pantoprazole sodium in both pure powder form and formulations. The high performance liquid chromatography (HPLC) method uses a C-18 column (250\u00a0mm \u00d7 4.6\u00a0mm, 5\u00a0\u03bcm) with a mobile phase consisting of 0.05\u00a0M disodium hydrogen phosphate and methanol in a 40:60% v\/v ratio. The flow rate is maintained at 1.0\u00a0mL\/min. On a layer of silica gel 60F254 with an aluminum backing, the high performance thin layer chromatography (HPTLC) separation was carried out with ethyl acetate and methanol (8: 1.5\u00a0v\/v) as the mobile phase. With a mean recovery of 100.54% and 99.55% for ASP and PNT, respectively, quantification was accomplished using the HPLC method with UV detection at 286\u00a0nm over the concentration range of 0.1\u20130.6\u00a0g\/mL for PNT and 0.4\u20132.4\u00a0g\/mL for ASP. With a mean recovery of 99.44% and 99.01% for ASP and PNT, respectively, quantification was achieved using the HPTLC method with UV detection at 298\u00a0nm over the concentration range of 400\u20132400\u00a0ng\/spot for ASP and 100\u2013600\u00a0ng\/spot for PNT, respectively. The methods can be used for the simultaneous determination of ASP and PNT in pure powder form and formulations as they are simple, accurate and sensitive.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae012","type":"journal-article","created":{"date-parts":[[2024,3,30]],"date-time":"2024-03-30T04:31:18Z","timestamp":1711773078000},"page":"477-482","source":"Crossref","is-referenced-by-count":4,"title":["Development and Validation of Chromatographic Methods for Simultaneous Estimation of Aspirin and Pantoprazole Sodium in Pure and Mixture Form"],"prefix":"10.1093","volume":"62","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-3049-0664","authenticated-orcid":false,"given":"Chandni","family":"Chandarana","sequence":"first","affiliation":[{"name":"Department of Quality Assurance and Pharmaceutical Chemistry, SSR College of Pharmacy , Sayli, Silvassa 396230 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Heli","family":"Desai","sequence":"additional","affiliation":[{"name":"Department of Quality Assurance and Pharmaceutical Chemistry, SSR College of Pharmacy , Sayli, Silvassa 396230 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ayushi","family":"Chauhan","sequence":"additional","affiliation":[{"name":"Department of Quality Assurance and Pharmaceutical Chemistry, SSR College of Pharmacy , Sayli, Silvassa 396230 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Vijaykumar","family":"Parmar","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Sciences, Sardar Patel University , Vallabh Vidyanagar 388120, Anand, Gujarat , India"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-9323-0179","authenticated-orcid":false,"given":"Parixit","family":"Prajapati","sequence":"additional","affiliation":[{"name":"Department of Quality Assurance and Pharmaceutical Chemistry, SSR College of Pharmacy , Sayli, Silvassa 396230 , India"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,3,28]]},"reference":[{"key":"2024060202562868200_ref1","doi-asserted-by":"crossref","first-page":"438","DOI":"10.1080\/22297928.2017.1326841","article-title":"HPLC method for the simultaneous estimation of aspirin and omeprazole in their new combination","volume":"7","author":"Nassar","year":"2017","journal-title":"Analytical Chemistry Letters"},{"issue":"1","key":"2024060202562868200_ref2","doi-asserted-by":"crossref","first-page":"227","DOI":"10.1161\/STR.0b013e3181f7d043","article-title":"Guidelines for the prevention of stroke in patients with stroke or transient ischemic attack: A guideline for healthcare professionals from the American Heart Association","volume":"42","author":"Furie","year":"2011","journal-title":"Stroke"},{"issue":"3","key":"2024060202562868200_ref3","doi-asserted-by":"crossref","first-page":"206","DOI":"10.1016\/j.jpha.2012.01.001","article-title":"High performance liquid chromatography mass spectrometric method for the simultaneous quantification of pravastatin and aspirin in human plasma: Pharmacokinetic application","volume":"2","author":"Polagani","year":"2012","journal-title":"Journal of Pharmaceutical Analysis"},{"issue":"4","key":"2024060202562868200_ref4","doi-asserted-by":"crossref","first-page":"646","DOI":"10.1016\/j.jpba.2010.10.002","article-title":"Quantitative HPLC analysis of mebeverine, mesalazine, sulphasalazine and dispersible aspirin stored in a Venalink monitored dosage system with co-prescribed medicines","volume":"54","author":"Elmasry","year":"2011","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"issue":"1","key":"2024060202562868200_ref5","first-page":"273","article-title":"Determination of platinum in vegetable samples using preconcentration cloud point extraction with flame atomic absorption spectrometry","volume":"105","author":"Rekha","year":"2007","journal-title":"Food Chemistry"},{"issue":"16","key":"2024060202562868200_ref6","doi-asserted-by":"crossref","first-page":"2944","DOI":"10.1080\/00032710802440574","article-title":"Absorptiometric determination of acetylsalicylic acid in aqueous ethanolic solution","volume":"41","author":"Iwunze","year":"2008","journal-title":"Analytical Letters"},{"key":"2024060202562868200_ref7","doi-asserted-by":"crossref","DOI":"10.1007\/978-3-030-17971-7_85","article-title":"UV-VIS determination of acetylsalicylic acid in aspirin tablets using different solvents and conditions","volume-title":"CMBEBIH 2019","author":"Daci\u0107","year":"2020"},{"key":"2024060202562868200_ref8","doi-asserted-by":"crossref","first-page":"65","DOI":"10.1016\/j.talanta.2005.02.004","article-title":"Solid-phase spectrofluorimetric determination of acetylsalicylic acid and caffeine in pharmaceutical preparations using partial least-squares multivariate calibration","volume":"67","author":"Moreira","year":"2005","journal-title":"Talanta"},{"key":"2024060202562868200_ref9","doi-asserted-by":"crossref","first-page":"8638","DOI":"10.1016\/j.electacta.2010.07.093","article-title":"Simultaneous voltametric determination of acetaminophen, aspirin and caffeine using an in-situ surfactant-modified multiwalled carbon nanotube paste electrode","volume":"55","author":"Sanghavi","year":"2010","journal-title":"Electrochimica Acta"},{"key":"2024060202562868200_ref10","doi-asserted-by":"crossref","first-page":"360","DOI":"10.1590\/S0103-50532009000200022","article-title":"Square-wave voltametric determination of acetylsalicylic acid in pharmaceutical formulations using a boron-doped diamond electrode without the need of previous alkaline hydrolysis step","volume":"20","author":"Sartori","year":"2009","journal-title":"Journal of Brazilian Chemical Society"},{"key":"2024060202562868200_ref11","volume-title":"Pantoprazole Sodium Monograph for Professionals-Drugs.com","year":"2022"},{"key":"2024060202562868200_ref12","doi-asserted-by":"crossref","first-page":"45","DOI":"10.1016\/S0731-7085(99)00275-7","article-title":"Spectrophotometric methods for the determination of lansoprazole and pantoprazole sodium sesquihydrate","volume":"22","author":"Moustafa","year":"2000","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"key":"2024060202562868200_ref13","first-page":"1984","article-title":"Spectrophotometric estimation of pantoprazole in tablet dosage form","volume":"6","author":"Vetrichelvi","year":"2008","journal-title":"International Journal of Chemical Science"},{"key":"2024060202562868200_ref14","doi-asserted-by":"crossref","DOI":"10.1098\/rsos.170324","article-title":"Development of a novel and cost-effective redox sensor for voltammetric determination of pantoprazole sodium during pharmacokinetic studies","volume":"4","author":"Khashaba","year":"2017","journal-title":"Royal Society Open Science"},{"issue":"4","key":"2024060202562868200_ref15","first-page":"793","article-title":"Development and validation of HPLC method for determination of pantoprazole in pantoprazole pellets","volume":"4","author":"Ognjenka","year":"2013","journal-title":"International Journal of Pharmacy Teaching & Practices"},{"issue":"5","key":"2024060202562868200_ref16","first-page":"34","article-title":"RP-\u00adHPLC method for the estimation of Pantoparzole sodium in bulk and in dosage forms","volume":"2","author":"Samanta","year":"2011","journal-title":"International Journal of Chemical and Analytical Science"},{"issue":"2","key":"2024060202562868200_ref17","first-page":"4253","article-title":"Development and validation of HPLC method for simultaneous estimation of cinitapride and pantoprazole in pharmaceutical dosage form","volume":"4","author":"Patel","year":"2012","journal-title":"International Journal of Pharmacy and Technology"},{"key":"2024060202562868200_ref18"},{"key":"2024060202562868200_ref19","doi-asserted-by":"crossref","first-page":"137","DOI":"10.2147\/JMDH.S54324","article-title":"Gastrointestinal ulcers, role of aspirin, and clinical outcomes: Pathobiology, diagnosis, and treatment","volume":"7","author":"Cryer","year":"2014","journal-title":"Journal of Multidisciplinary Healthcare"},{"issue":"10","key":"2024060202562868200_ref20","first-page":"76","article-title":"Development and validation of HPTLC method for simultaneous quantification of aspirin and omeprazole in bulk and marketed formulation","volume":"11","author":"Chandarana","year":"2019","journal-title":"Journal of Chemical and Pharmaceutical Research"},{"issue":"8","key":"2024060202562868200_ref21","first-page":"3777","article-title":"Quantitative simultaneous estimation of aspirin and omeprazole by RP-HPLC method implementing AQBD approach in pharmaceutical dosage form","volume":"10","author":"Chandarana","year":"2019","journal-title":"International Journal of Pharmaceutical Sciences and Research"},{"issue":"3","key":"2024060202562868200_ref22","first-page":"1575","article-title":"Analytical method development and validation for the determination of pantoprazole and aspirin using reverse phase HPLC method in combined tablet dosage form","volume":"11","author":"Shahid Afridi","year":"2022","journal-title":"World Journal of Pharmaceutical Research"},{"key":"2024060202562868200_ref23","volume-title":"The International Council for Harmonisation of Technical Requirements for Pharmaceuticals for Human Use (ICH), IFPMA","author":"ICH guideline, Q2 (R1)","year":"2005"},{"key":"2024060202562868200_ref24","volume-title":"Center for Drug Evaluation and Research. Reviewer guidance: validation of chromatographic methods","author":"Food and Drug Administration","year":"1994"}],"container-title":["Journal of Chromatographic Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/62\/5\/477\/58033543\/bmae012.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/62\/5\/477\/58033543\/bmae012.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2024,6,2]],"date-time":"2024-06-02T02:56:45Z","timestamp":1717297005000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/chromsci\/article\/62\/5\/477\/7636971"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2024,3,28]]},"references-count":24,"journal-issue":{"issue":"5","published-online":{"date-parts":[[2024,3,28]]},"published-print":{"date-parts":[[2024,5,31]]}},"URL":"https:\/\/doi.org\/10.1093\/chromsci\/bmae012","relation":{},"ISSN":["0021-9665","1945-239X"],"issn-type":[{"value":"0021-9665","type":"print"},{"value":"1945-239X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2024,5]]},"published":{"date-parts":[[2024,3,28]]}}}