{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,10,8]],"date-time":"2026-10-08T18:44:27Z","timestamp":1791485067398,"version":"4.3.4"},"reference-count":27,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,5,4]],"date-time":"2024-05-04T00:00:00Z","timestamp":1714780800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Design of experiment is an efficient and cost-effective tool to optimize the chromatographic separation of a multicomponent mixture. The central composite design was conducted to develop and optimize a green high performance liquid chromatography (HPLC) method for simultaneous quantitation of a quaternary mixture of paracetamol, chlorpheniramine maleate, caffeine and ascorbic acid in their pharmaceutical dosage form as well as the determination of their dissolution profile. A five-level three-factor model was performed to investigate the effect of mobile phase composition, pH and flow rate on enhanced resolution and short run time. Analysis was performed using a Kinitex EVO C18 column and a mobile phase composed of methanol: 0.02\u00a0M phosphate buffer pH\u00a03.3 (34:66, v\/v) at 1.0\u00a0mL\/min using photodiode array detection. Optimum chromatographic separation was achieved in &amp;lt;6\u00a0min with a desirability of 0.999. Linearity was achieved over a range of 1.00\u2013300.00, 1.00\u201350.00, 2.00\u201350.00 and 2.00\u2013100.00\u00a0\u03bcg\/mL for paracetamol, chlorpheniramine maleate, caffeine and ascorbic acid, respectively, with a limit of detection (&amp;lt;0.1\u00a0\u03bcg\/mL). The greenness profile was evaluated using the analytical eco-scale and Analytical GREEnness Metric Approach with values of 81 and 0.77, respectively.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae024","type":"journal-article","created":{"date-parts":[[2024,4,19]],"date-time":"2024-04-19T19:10:51Z","timestamp":1713553851000},"source":"Crossref","is-referenced-by-count":11,"title":["Development of Response Surface Approach for Determination of Paracetamol, Chlorpheniramine Maleate, Caffeine and Ascorbic Acid by Green HPLC Method: A Desirability-Based Optimization"],"prefix":"10.1093","author":[{"given":"Aya A","family":"Mouhamed","sequence":"first","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Basma M","family":"Eltanany","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Nadia M","family":"Mostafa","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Ahmed H","family":"Nadim","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini St., Cairo 11562 , Egypt"}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2024,5,4]]},"reference":[{"key":"2024050501352699900_ref1","volume-title":"Goodman & Gilman\u2019s The Pharmacological Basis of Therapeutics","author":"Brunton","year":"2011","edition":"12th"},{"key":"2024050501352699900_ref2","author":"The British Pharmacopoeia","year":"2016"},{"key":"2024050501352699900_ref3","first-page":"165","volume":"29","author":"Yang","year":"2008","journal-title":"The Journal of Korean Medicine"},{"key":"2024050501352699900_ref4","doi-asserted-by":"crossref","first-page":"106","DOI":"10.1016\/j.ab.2007.11.011","article-title":"Development of an HPLC\u2013MS\/MS method for the selective determination of paracetamol metabolites in mouse urine","volume":"374","author":"Hewavitharana","year":"2008","journal-title":"Analytical 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