{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,13]],"date-time":"2026-05-13T21:27:55Z","timestamp":1778707675349,"version":"3.51.4"},"reference-count":26,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,6,12]],"date-time":"2024-06-12T00:00:00Z","timestamp":1718150400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>A sensitive, specific, reliable and low-cost LC-UV method has been developed and validated for simultaneous quantification of brimonidine tartrate (BM) and brinzolamide (BZ) in rabbit aqueous humor (AH) in the presence of N-desethyl-brinzolamide (NDBZ); BZ is a major degradation product, and it is also considered to be its major metabolite. Dorzolamide hydrochloride (DZ) was used as an internal standard (IS). The analytes were extracted from rabbit AH samples by a simple pre-treatment utilizing ZnSO4.7H2O as a deproteinizing agent. The analytes were separated on a cyanopropyl Waters column (4.6\u2009\u00d7\u2009200\u00a0mm, 5\u00a0\u03bcm) with an isocratic mobile phase consisting of 25\u00a0mM ammonium acetate pH\u00a04.5 (adjusted with 85% phosphoric acid):methanol:acetonitrile (94:4.5:1.5, v\/v) at a flow rate of 1.0\u00a0mL\u00a0min\u22121. The detection was done at 254\u00a0nm. The lower limit of quantification in rabbit AH was 100.0\u00a0ng\u00a0mL\u22121. The method was validated according to EMA guidelines. The method was confirmed to be accurate, precise and linear over a range of 100.0\u20131000.0\u00a0ng\u00a0mL\u22121 for BM and BZ. The method developed herein is simple, safe, eco-friendly, rapid and accurately applied for the quantification of BM and BZ, along with the successful separation of NDBZ, which is considered as a potential irritant degradation product of BZ.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae040","type":"journal-article","created":{"date-parts":[[2024,6,1]],"date-time":"2024-06-01T05:56:52Z","timestamp":1717221412000},"source":"Crossref","is-referenced-by-count":4,"title":["A Simple Validated LC-UV Method for the Simultaneous Determination of Brimonidine and Brinzolamide in the Presence of Brinzolamide\u2019s Degradation Product (Major Metabolite) in Rabbit Aqueous Humor"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0009-0006-1668-7523","authenticated-orcid":false,"given":"Marwa A","family":"Wahab Mubarak","sequence":"first","affiliation":[{"name":"Pharmaceutical Chemistry Department, Egyptian Drug Authority (EDA) , Wezzart Elzeraa Street, Giza 35521 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"M Abdul-Azim","family":"Mohammad","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ramzia I","family":"El-Bagary","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Ehab","family":"Elkady","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]},{"name":"Cairo University , Faculty of Pharmacy, , Kasr El-Aini Street, Cairo 11562 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Nisreen F","family":"Abo-Talib","sequence":"additional","affiliation":[{"name":"Pharmaceutical Chemistry Department, Egyptian Drug Authority (EDA) , Wezzart Elzeraa Street, Giza 35521 ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,6,12]]},"reference":[{"key":"2025011412165266100_ref1","doi-asserted-by":"crossref","first-page":"3","DOI":"10.1016\/j.mjafi.2015.12.001","article-title":"Glaucoma: The \u201cblack hole\u201d of irreversible blindness","volume":"72","author":"Parihar","year":"2016","journal-title":"Medical Journal Armed Forces India"},{"key":"2025011412165266100_ref2","doi-asserted-by":"crossref","first-page":"109","DOI":"10.1016\/j.jchromb.2016.04.012","article-title":"Simultaneous determination of 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