{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,13]],"date-time":"2026-03-13T17:24:32Z","timestamp":1773422672373,"version":"3.50.1"},"reference-count":24,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2024,6,27]],"date-time":"2024-06-27T00:00:00Z","timestamp":1719446400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Ministry of Food and Drug Safety of the Republic of Korea","award":["21201MFDS306"],"award-info":[{"award-number":["21201MFDS306"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Recently, the demand for respiratory disease-related products has surged due to the influence of coronavirus disease 2019, prompting warnings about illegal dietary supplements containing unauthorized substances. Additionally, adulterated dietary supplements are continuously detected in open markets, posing significant public health safety problem. In this study, we developed and validated an analytical method for 11 respiratory drug substances using liquid chromatography\u2013electrospray ionization\u2013tandem mass spectrometry (LC\u2013ESI\u2013MS\/MS) and proposed optimal conditions for LC\u2013quadrupole time-of-flight MS (LC\u2013QTOF-MS) to determine the fragmentation patterns of each substance. This method underwent thorough validation considering specificity, linearity, limits of detection and quantification, accuracy, precision, matrix effect, stability, etc. All results met international guidelines. These validated methods were applied to 52 dietary supplements advertised for treating respiratory diseases and enhancing respiratory function, among which one sample was found to contain 313.7\u00a0mg\/g of theobromine. This determination was made by comparing the product ion ratios with the standards and subsequent quantification. To re-confirm the detected substances, their fragmentation patterns were compared with those of the standards using LC\u2013QTOF-MS. In conclusion, the mass-based information, coupled with the LC\u2013ESI\u2013MS\/MS method development, can be successfully applied to rapidly identify 11 respiratory drug substances in illegal dietary supplements used for respiratory disease treatment. The developed simultaneous detection method contributes to public health and safety improvements.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae044","type":"journal-article","created":{"date-parts":[[2024,6,14]],"date-time":"2024-06-14T14:21:00Z","timestamp":1718374860000},"source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous Detection Method of 11 Respiratory Drug Substances Including Theobromine, Oxymetazoline, etc. in Adulterated Dietary Supplements Using Liquid Chromatography\u2013Electrospray Ionization\u2013Tandem Mass Spectrometry and Liquid Chromatography\u2013Quadrupole Time-of-Flight Mass Spectrometry Analysis"],"prefix":"10.1093","volume":"63","author":[{"given":"Mi Jin","family":"Kim","sequence":"first","affiliation":[{"name":"Advanced Analysis Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 363-700 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5255-7906","authenticated-orcid":false,"given":"Hwan Seong","family":"Choi","sequence":"additional","affiliation":[{"name":"Advanced Analysis Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 363-700 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Hyunil","family":"Shin","sequence":"additional","affiliation":[{"name":"Advanced Analysis Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 363-700 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0001-5337-5648","authenticated-orcid":false,"given":"Ji Hyun","family":"Lee","sequence":"additional","affiliation":[{"name":"Advanced Analysis Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 363-700 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-5247-3411","authenticated-orcid":false,"given":"Nam Sook","family":"Kim","sequence":"additional","affiliation":[{"name":"Advanced Analysis Division, National Institute of Food and Drug Safety Evaluation, Ministry of Food and Drug Safety , Osong Health Technology Administration Complex, 187 Osongsaengmyeong2-ro, Osongeup, Heungdeok-gu, Cheongju-si, Chungcheongbuk-do 363-700 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