{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,10]],"date-time":"2026-01-10T01:08:49Z","timestamp":1768007329392,"version":"3.49.0"},"reference-count":19,"publisher":"Oxford University Press (OUP)","license":[{"start":{"date-parts":[[2024,11,14]],"date-time":"2024-11-14T00:00:00Z","timestamp":1731542400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>In this research study, a simple, rapid, sensitive, and cost-effective Reverse Phase High Performance Liquid Chromatography method was developed and validated for determination of Cetirizine and Levocetirizine in human plasma. The drugs were separated on an ACE Generix100\u20135 C18 RP (250\u2009\u00d7\u20094.6\u00a0mm, 5\u00a0\u03bcm) column, using Acetonitrile and Distilled water pH\u00a07 (59:41\u00a0v\/v) as mobile phase with a flow rate of 1.5\u00a0mL\/min, retention time of 3.39\u2009\u00b1\u20090.02\u00a0min, detection wavelength of 230\u00a0nm, and column oven temperature at 26\u00b0C. The drug was extracted from human plasma using Methanol, the percent recovery was 97%. The LOD and LOQ for Cetirizine and Levocetirizine were 0.5\u00a0ng and 1\u00a0ng. The method was linear over the concentration range of 0.5\u20131000\u00a0ng \/mL for Cetirizine and Levocetirizine. The method was applied for analysis of drugs in plasma following oral administration of Cetirizine (10\u00a0mg) and Levocetirizine (5\u00a0mg) in smokers and nonsmokers of Pashtun ethnicity belonging from different districts of Khyber Pakhtunkhwa, Pakistan. The difference between Cetirizine plasma levels in smokers and non-smokers was significant with p -value 0.0279.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae058","type":"journal-article","created":{"date-parts":[[2024,11,15]],"date-time":"2024-11-15T08:47:43Z","timestamp":1731660463000},"source":"Crossref","is-referenced-by-count":1,"title":["Development and Validation of Rapid and Cost-Effective RP-HPLC\/UV Method for Determination of Cetirizine\/Levocetirizine in Blood Plasma of Smokers and Non Smokers: a Two Cohort Comparative Study"],"prefix":"10.1093","author":[{"given":"Mashal","family":"Khattak","sequence":"first","affiliation":[{"name":"Department of Pharmacy, University of Peshawar , Peshawar 25000,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Zafar","family":"Iqbal","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Peshawar , Peshawar 25000,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0003-3880-5579","authenticated-orcid":false,"given":"Asif","family":"Jan","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Peshawar , Peshawar 25000,","place":["Pakistan"]},{"name":"District Headquarter Hospital (DHQH) Charsadda , Charsadda 24430,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Niaz","family":"Ali","sequence":"additional","affiliation":[{"name":"Department of Pharmacology , College of Medicine, , Shaqra 11961,","place":["Kindom of Saudi Arabia"]},{"name":"Shaqra University , College of Medicine, , Shaqra 11961,","place":["Kindom of Saudi Arabia"]},{"name":"Institute of Basic Medical Sciences, Khyber Medical University , Peshawar 25000,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Fazli","family":"Nasir","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, University of Peshawar , Peshawar 25000,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Naila","family":"Shahbaz","sequence":"additional","affiliation":[{"name":"Department of Pharmacy, Sarhad University of Science & Information Technology , Peshawar 25000,","place":["Pakistan"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,11,14]]},"reference":[{"key":"2024111508471474900_ref1","first-page":"1","article-title":"Determination and quantification of cetirizine HCl in dosage formulations by RP-HPLC","volume":"1","author":"Arayne","year":"2005","journal-title":"Sciences"},{"issue":"1","key":"2024111508471474900_ref2","first-page":"1","article-title":"A validated stability-indicating HPLC assay method 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