{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,3,28]],"date-time":"2026-03-28T08:04:45Z","timestamp":1774685085965,"version":"3.50.1"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"1","license":[{"start":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T00:00:00Z","timestamp":1734566400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Xinglin Young Talent program of the Shanghai University of TCM","award":["U182050"],"award-info":[{"award-number":["U182050"]}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81929004"],"award-info":[{"award-number":["81929004"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/501100001809","name":"National Natural Science Foundation of China","doi-asserted-by":"publisher","award":["81973876"],"award-info":[{"award-number":["81973876"]}],"id":[{"id":"10.13039\/501100001809","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,9]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Chuanxiong Rhizoma (Chuanxiong), a traditional Chinese medicine, has been widely used to treat various nervous and cardiovascular system-related conditions. Its active components, senkyunolide A (SA) and 3-n-butylphthalide (NBP), have been proven effective in treating nervous system diseases. A new method was established based on microdialysis coupled with ultra-performance liquid chromatography-tandem mass spectrometry (UPLC-MS\/MS) to estimate the concentrations of these components in brain extracellular fluid. Chromatographic separation was achieved using an Acquity UPLC BEH C8 column (2.1\u2009\u00d7\u2009100\u00a0mm, 1.7\u00a0\u03bcm) with acetonitrile and 0.1% formic acid as mobile phase. The calibration curves of SA and NBP were linear in the concentration ranges of 0.25\u2013100.00 and 0.12\u201348.00\u00a0ng\/mL, respectively, with a correlation coefficient above 0.9992. All validation parameters, including intra- and inter-day precision, accuracy, matrix effect and stability, were within the acceptance limits of bioanalytical guidelines. The validated method was successfully applied to study the pharmacokinetics of SA and NBP in rat brain microdialysis after oral administration of Chuanxiong extracts. The results showed that both components penetrated the brain and reached maximum concentrations in the microdialysates of 72.31 and 9.93\u00a0ng\/mL at 1.50 and 1.58\u00a0h, respectively.<\/jats:p>","DOI":"10.1093\/chromsci\/bmae059","type":"journal-article","created":{"date-parts":[[2024,12,19]],"date-time":"2024-12-19T07:44:13Z","timestamp":1734594253000},"source":"Crossref","is-referenced-by-count":2,"title":["UPLC-MS\/MS Combined with Microdialysis for Determination of Two Active Components of <i>Chuanxiong Rhizoma<\/i> Extracts in Rat Brain: Application in Pharmacokinetic Study"],"prefix":"10.1093","volume":"63","author":[{"given":"Yu","family":"Wang","sequence":"first","affiliation":[{"name":"Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine , Shanghai 201203 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Liang","family":"Jin","sequence":"additional","affiliation":[{"name":"Xinhua Hospital Affiliated to Shanghai Jiaotong University School of Medicine , Shanghai 200092 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Xinhua","family":"Liu","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine , Shanghai 201203 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bing","family":"Shu","sequence":"additional","affiliation":[{"name":"Spine Institute, Shanghai University of Traditional Chinese Medicine , Shanghai 200092 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jia","family":"Xu","sequence":"additional","affiliation":[{"name":"Fudan University Department of Pharmacy, Fudan University Shanghai Cancer Center, Department of Oncology, Shanghai Medical College, , Shanghai 200032 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-9979-5645","authenticated-orcid":false,"given":"Lingyun","family":"Pan","sequence":"additional","affiliation":[{"name":"Experiment Center for Science and Technology, Shanghai University of Traditional Chinese Medicine , Shanghai 201203 ,","place":["China"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2024,12,19]]},"reference":[{"key":"2025010912420676300_ref1","doi-asserted-by":"publisher","first-page":"1731","DOI":"10.1142\/S0192415X2050086X","article-title":"Progress in traditional Chinese medicine for the treatment of migraine","volume":"48","author":"Huang","year":"2020","journal-title":"The American Journal of Chinese Medicine"},{"key":"2025010912420676300_ref2","doi-asserted-by":"crossref","first-page":"26","DOI":"10.1186\/s13020-016-0098-5","article-title":"The variation in the major constituents of the 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