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All target components were completely separated in a C18 column with isocratic elution of 5% acetonitrile solution containing 8\u00a0mM HClO4 and 0.20\u00a0mM 1-octanesulfonic acid as an ion pairing reagent. This method showed limits of detection of 0.03\u20130.1\u00a0ng and limits of quantification of 0.10\u20130.3\u00a0ng with linear regression coefficients of 0.9998\u20131.0000. All inter-day and intra-day precision values were below 9.58%, and the average recoveries were 93.71\u2013109.82% for mouse striatum samples. The mean levels of the seven components in striatal brain tissue in a mouse model of Parkinson\u2019s disease decreased by 2\u201312 times compared to those of a control group. In particular, the decrease in dopamine, 5-hydroxyindolacetic acid and 3,4-dihydroxyphenylacetic acid was statistically significant (P\u2009&amp;lt;\u20090.05). Orthogonal partial least squares discriminant analysis confirmed that the seven components are useful biomarkers. The significance of the developed method lies in its ability to simultaneously analyze seven biogenic amines related to Parkinson\u2019s disease by combining two detectors, offering a simple and cost-effective approach for clinical and biological labs.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf004","type":"journal-article","created":{"date-parts":[[2025,1,15]],"date-time":"2025-01-15T18:02:13Z","timestamp":1736964133000},"source":"Crossref","is-referenced-by-count":2,"title":["Simultaneous Quantification of Biogenic Amines and their Metabolites in Mice Tissue by Combining Ultraviolet and Integrated Pulsed Amperometric Detectors"],"prefix":"10.1093","volume":"63","author":[{"given":"Hae-Jee","family":"Yoon","sequence":"first","affiliation":[{"name":"Department of Integrated Drug Development and Natural Products Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Jin Hee","family":"Kim","sequence":"additional","affiliation":[{"name":"Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Myung Sook","family":"Oh","sequence":"additional","affiliation":[{"name":"Department of Integrated Drug Development and Natural Products Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]},{"name":"Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]},{"name":"Department of Oriental Pharmaceutical Science and Kyung Hee East-West Pharmaceutical Research Institute, College of Pharmacy, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Seon-Pyo","family":"Hong","sequence":"additional","affiliation":[{"name":"Department of Integrated Drug Development and Natural Products Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]},{"name":"Department of Biomedical and Pharmaceutical Sciences, Graduate School, Kyung Hee University , 26, Kyungheedae-ro, Dongdaemun-gu, Seoul, 02447 ,","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-7133-5401","authenticated-orcid":false,"given":"Ha-Jeong","family":"Kwon","sequence":"additional","affiliation":[{"name":"Division of Chemical and Material Metrology, Korea Research Institute of Standards and Science , 267, Gajeong-ro, Yuseong-gu, Daejeon, 34113","place":["Republic of Korea"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,1,15]]},"reference":[{"key":"2025011518015040800_ref1","doi-asserted-by":"publisher","first-page":"235","DOI":"10.1016\/S1474-4422(06)70373-8","article-title":"National Institute for clinical excellence; non-motor symptoms of Parkinson's disease: Diagnosis and management","volume":"5","author":"Chaudhuri","year":"2006","journal-title":"The Lancet Neurology"},{"key":"2025011518015040800_ref2","doi-asserted-by":"publisher","first-page":"532","DOI":"10.1002\/ana.20226","article-title":"Parkinsonian signs and substantia nigra neuron density in decendents elders without PD","volume":"56","author":"Ross","year":"2004","journal-title":"Annals of Neurology"},{"key":"2025011518015040800_ref3","doi-asserted-by":"publisher","first-page":"415","DOI":"10.1016\/0022-510x(73)90175-5","article-title":"Brain dopamine and the syndromes of Parkinson and Huntington. 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