{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,5]],"date-time":"2026-01-05T10:58:23Z","timestamp":1767610703289,"version":"3.41.2"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2025,1,14]],"date-time":"2025-01-14T00:00:00Z","timestamp":1736812800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"name":"Scientific Research Projects Units of Bezmialem Vak\u0131f University","award":["20210219"],"award-info":[{"award-number":["20210219"]}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,1,14]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>This study presents a combination of High Performance Liquid Chromatography (HPLC) and ultraviolet (UV) detection that provides the quantification of agnuside in human plasma specimens. Reverse-phase chromatographic separation was carried out with C18 column (150\u00a0mm\u2009\u00d7\u20094.6\u00a0mm\u2009\u00d7\u20095\u00a0\u03bcm), at 25\u00b0C with isocratic elution of the mobile phase containing methanol: 0.1% formic acid (35:65\u00a0v\/v) at 0.6\u00a0mL\/min flow rate. Experiments were carried out at a wavelength of 258\u00a0nm. The retention time of the analyte is 9.70\u2009\u00b1\u20090.01\u00a0min. The developed technique was validated based on the International Conference on Harmonization guideline. The correlation coefficient of the technique was 0.9915, and the calibration range was 5\u2013125\u00a0\u03bcg\/mL. The recovery value of the proposed method was found to be 101.4%, and the precision of the method was calculated as 6.35 with the highest RSD% value. A pharmacokinetic study was performed by administering agnuside to a healthy volunteer.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf007","type":"journal-article","created":{"date-parts":[[2025,1,21]],"date-time":"2025-01-21T11:31:02Z","timestamp":1737459062000},"source":"Crossref","is-referenced-by-count":1,"title":["Quantification of Agnuside in Human Plasma with a Novel High-Performance Liquid Chromatographic Method and Pharmacokinetic Study"],"prefix":"10.1093","volume":"63","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-0933-881X","authenticated-orcid":false,"given":"Derya","family":"Egeli","sequence":"first","affiliation":[{"name":"Faculty of Pharmacy, Department of Pharmacognosy, Bezmialem Vakif University Vatan Street , Fatih, Istanbul 34093 ,","place":["Turkey"]},{"name":"Department of Pharmacognosy and Natural Products Chemistry, Institute of Health Sciences Bezmialem Vakif University , Fatih, Istanbul 34093 ,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-2547-6086","authenticated-orcid":false,"given":"Gizem","family":"Tiris","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry, Bezmialem Vakif University Fatih , Istanbul 34093 ,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1871-017X","authenticated-orcid":false,"given":"Evrim","family":"Kepekci Tekkeli","sequence":"additional","affiliation":[{"name":"Faculty of Pharmacy, Department of Analytical Chemistry, Istanbul Health and Technology University , Istanbul 34469 ,","place":["Turkey"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,1,21]]},"reference":[{"issue":"13","key":"2025012111305680500_ref1","doi-asserted-by":"crossref","first-page":"1201","DOI":"10.1080\/14786410802696494","article-title":"A new iridoid glycoside fromVitex negundoLinn (Verbenacea)","volume":"23","author":"Sharma","year":"2009","journal-title":"Natural Product Research"},{"issue":"4","key":"2025012111305680500_ref2","doi-asserted-by":"crossref","first-page":"655","DOI":"10.1248\/cpb.46.655","article-title":"Pharmacologically active components of Viticis fructus (Vitex rotundifolia). 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