{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,8,17]],"date-time":"2026-08-17T06:14:08Z","timestamp":1786947248685,"version":"build-2736575974"},"reference-count":32,"publisher":"Oxford University Press (OUP)","issue":"4","license":[{"start":{"date-parts":[[2025,3,27]],"date-time":"2025-03-27T00:00:00Z","timestamp":1743033600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,3,27]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ephedrine is one component of a popular traditional Chinese multi-plant formulation used to treat asthma, fever, coughs and lately COVID-19. The aim of this study is to develop and validate a simple and rapid quali-quantitative High Performance Thin Layer Chromatography (HPTLC) densitometry method to detect ephedrine, the most abundant alkaloid of Ephedra plant, and to analyze free sale ephedra-based capsules. This quick, simple and low requirement method is cost-effective and allows screening up to 19 samples all together in a single layer. During the development of this method, an extraction solvent mixture composed of methanol\/water (2:1, v\/v) and a mobile phase constituted of ammonia\/methanol\/dichloromethane (1:10:40, v\/v\/v) gave the best results. The identity and purity of ephedrine peak were confirmed by matching Rf value and UV-spectrum with its reference standard. Lastly, samples were quantitated by densitometry at 500\u00a0nm after ninhydrin derivatization. Linearity was obtained in the concentration range of 0.062\u20130.146\u00a0\u03bcg\/band. Limit of detection and limit of quantification (LOQ) were found to be 0.0020\u00a0\u03bcg\/band and 0.0067\u00a0\u03bcg\/band, respectively. This technique is useful to screen multiple samples at the same time with a limited use of solvents and reagents so that it can be considered a fast and simple procedure.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf020","type":"journal-article","created":{"date-parts":[[2025,4,11]],"date-time":"2025-04-11T09:25:01Z","timestamp":1744363501000},"source":"Crossref","is-referenced-by-count":2,"title":["An High Performance Thin Layer Chromatography (HPTLC) Validated Method for the Determination of Ephedrine Alkaloids in Multi-Plant Formulations"],"prefix":"10.1093","volume":"63","author":[{"given":"Simonetta","family":"Gemma","sequence":"first","affiliation":[{"name":"Centro Nazionale per la Ricerca e Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanit\u00e0 , Viale Regina Elena 299, Roma 00161 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Giuseppina","family":"Multari","sequence":"additional","affiliation":[{"name":"Centro Nazionale per la Ricerca e Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanit\u00e0 , Viale Regina Elena 299, Roma 00161 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Francesca R","family":"Gallo","sequence":"additional","affiliation":[{"name":"Centro Nazionale per la Ricerca e Valutazione Preclinica e Clinica dei Farmaci, Istituto Superiore di Sanit\u00e0 , Viale Regina Elena 299, Roma 00161 ,","place":["Italy"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,4,11]]},"reference":[{"key":"2025041109245572400_ref1","volume-title":"Chinese herbal medicine Materia Medica","author":"Bensky","year":"1986","edition":"1st"},{"key":"2025041109245572400_ref2","first-page":"711","volume-title":"Pharmacognosy, phytochemistry, medicinal plants","author":"Bruneton","year":"1995"},{"issue":"8\u20139","key":"2025041109245572400_ref3","doi-asserted-by":"publisher","first-page":"895","DOI":"10.1016\/j.phytochem.2010.03.019","article-title":"Composition and stereochemistry of ephedrine alkaloids accumulation in Ephedra sinica Stapf","volume":"71","author":"Krizevski","year":"2010","journal-title":"Phytochemistry"},{"issue":"11","key":"2025041109245572400_ref4","doi-asserted-by":"publisher","first-page":"1494","DOI":"10.1248\/cpb.53.1494","article-title":"Chromatographic separation of (\u2212)-ephedrine and (+)-pseudoephedrine in the traditional Chinese medicinal preparation Jiketing granule","volume":"53","author":"Luhua","year":"2005","journal-title":"Chemical & Pharmaceutical Bulletin"},{"issue":"5","key":"2025041109245572400_ref5","doi-asserted-by":"publisher","first-page":"1633","DOI":"10.1016\/j.jpba.2006.02.043","article-title":"A rapid and simple procedure for the determination of ephedrine alkaloids in dietary supplements by gas chromatography-mass spectrometry","volume":"41","author":"Marchei","year":"2006","journal-title":"Journal of Pharmaceutical and Biomedical Analysis"},{"key":"2025041109245572400_ref6","doi-asserted-by":"publisher","first-page":"1441","DOI":"10.1136\/bmj.312.7044.1441","article-title":"Herbal stimulant causes US deaths","volume":"312","author":"Josefson","year":"1996","journal-title":"BMJ"},{"key":"2025041109245572400_ref7","first-page":"81","volume-title":"Divieto di prescrizione e di esecuzione di preparazioni magistrali a scopo dimagrante contenenti le sostanze medicinali efedrina e pseudoefedrina","author":"Salute","year":"2017"},{"key":"2025041109245572400_ref8","volume-title":"Revisione della lista Dei farmaci, delle sostanze biologicamente o farmacologicamente attive e delle pratiche mediche, il cui impiego \u00e8 considerato doping, ai sensi della legge 14 dicembre 2000e, n. 376","author":"Salute"},{"key":"2025041109245572400_ref9","doi-asserted-by":"publisher","first-page":"828","DOI":"10.1016\/j.microc.2019.02.012","article-title":"Novel determination of a new antiviral combination; 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