{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,7,30]],"date-time":"2025-07-30T11:47:39Z","timestamp":1753876059451,"version":"3.41.2"},"reference-count":35,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,5,1]],"date-time":"2025-05-01T00:00:00Z","timestamp":1746057600000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Ensuring the accurate measurement of the active components in herbal medicine is critical in maintaining their clinical effectiveness. One quality-controlled medicine is the rosemary\u2013pepper tincture, a hydroalcoholic extract of Lippia sidoides leaves that contains the active ingredient, thymol. It is used as an antiseptic to treat injuries. Thus, a validated method for standardizing the active component was developed to extract the thymol and eliminate polar and nonpolar interferents, thereby extending the lifespan of the chromatographic column. To evaluate the adsorption of thymol into the flow, the adsorption data were adjusted using the Thomas model to obtain the maximum amount of adsorption, 4.33\u00a0mg\/g. Factorial planning revealed that the best conditions for the adsorption and desorption of thymol were 1.4\u00a0mL of tincture in an SPE-C18 column and 2\u00a0ml of methanol\/water (80\/20) as eluent. Subsequently, the thymol was quantified using HPLC-UV spectroscopy, and the calibration curves were linear in the working range of 0.1\u20131.0\u00a0mg\/mL (R2\u00a0=\u20090.997). The limits of detection and quantification were 0.02 and 0.06\u00a0mg\/mL, respectively. The precision and accuracy were 95.8\u2013103.8% at three different concentrations. Finally, the method proved robust against multiple applied variations and yielded satisfactory results within the analysis parameters.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf027","type":"journal-article","created":{"date-parts":[[2025,5,21]],"date-time":"2025-05-21T22:52:25Z","timestamp":1747867945000},"source":"Crossref","is-referenced-by-count":0,"title":["Development of a solid phase extraction-high\u2013performance liquid chromatography\u2013ultraviolet detector method for quality control of the rosemary\u2013pepper tincture, a phytotherapic produced by living pharmacies in Brazil"],"prefix":"10.1093","volume":"63","author":[{"given":"Geovanna","family":"Soldi","sequence":"first","affiliation":[{"name":"Instituto de Ci\u00eancias Exatas, Universidade Federal de Vi\u00e7osa, Campus Rio Parana\u00edba , Rio Parana\u00edba, MG 38810000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Bernardo Augusto de Freitas","family":"Dornelas","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Exatas, Universidade Federal de Vi\u00e7osa, Campus Rio Parana\u00edba , Rio Parana\u00edba, MG 38810000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Mario Ferreira Concei\u00e7\u00e3o","family":"Santos","sequence":"additional","affiliation":[{"name":"Departamento de Qu\u00edmica e F\u00edsica, Universidade Federal do Esp\u00edrito Santo, Alto Universit\u00e1rio S\/N , Bairro Guararema, Alegre, ES 29500-000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Giulia Stavrakas","family":"Miranda","sequence":"additional","affiliation":[{"name":"Departamento de Biologia, Universidade Federal do Esp\u00edrito Santo, Alto Universit\u00e1rio s\/n , Bairro Guararema, Alegre, ES 29500-000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Geraldo Humberto","family":"Silva","sequence":"additional","affiliation":[{"name":"Instituto de Ci\u00eancias Exatas, Universidade Federal de Vi\u00e7osa, Campus Rio Parana\u00edba , Rio Parana\u00edba, MG 38810000 ,","place":["Brazil"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,5,21]]},"reference":[{"year":"2014","author":"Minist\u00e9rio Da","key":"2025052118521663700_ref1"},{"key":"2025052118521663700_ref2","doi-asserted-by":"publisher","first-page":"1861","DOI":"10.1177\/1934578X1501001116","article-title":"General characteristics, phytochemistry and pharmacognosy of Lippia sidoides","volume":"10","author":"De Guimar\u00e3es","year":"2015","journal-title":"Natural Product Communications"},{"volume-title":"Caracteriza\u00e7\u00e3o e comportamento de acessos de Alecrim-Pimenta (Lippia sidoides Cham.) mantidos em banco ativo de germoplasma em S\u00e3o Crist\u00f3v\u00e3o\u2014SE","year":"2008","author":"Oliveira","key":"2025052118521663700_ref3"},{"key":"2025052118521663700_ref4","doi-asserted-by":"publisher","first-page":"598","DOI":"10.1021\/np50017a018","article-title":"Essential oils from Brazilian Verbenaceae genus Lippia","volume":"44","author":"Crayeiro","year":"1981","journal-title":"Journal of Natural Products"},{"key":"2025052118521663700_ref5","doi-asserted-by":"publisher","first-page":"349","DOI":"10.1590\/S0100-879X2007000300010","article-title":"Antimicrobial activity of the essential oil from Lippia sidoides, carvacrol and thymol against oral pathogens","volume":"40","author":"Botelho","year":"2007","journal-title":"Brazilian Journal of Medical and Biological Research"},{"issue":"1","key":"2025052118521663700_ref6","doi-asserted-by":"publisher","first-page":"283","DOI":"10.1016\/j.jep.2014.02.030","article-title":"Topical continuous use of Lippia sidoides Cham. 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