{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,6,15]],"date-time":"2026-06-15T12:15:20Z","timestamp":1781525720951,"version":"3.54.1"},"reference-count":37,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T00:00:00Z","timestamp":1748563200000},"content-version":"vor","delay-in-days":29,"URL":"https:\/\/creativecommons.org\/licenses\/by-nc\/4.0\/"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,5,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n               <jats:p>Current vitamin D quantification methods do not account for 25-hydroxyl epimers, which can falsely increase concentrations and mask actual deficiencies. Previously, we developed an ultra-high performance liquid chromatography\u2013tandem mass spectrometry method to measure 25(OH)D3, 3-epi-25(OH)D3 and 25(OH)D2; here, we extended this method to include 3-epi-25(OH)D2. Analytes were separated using a Shimadzu UPLC with a Kinetex F5 column (100\u2009\u00d7\u20092.1\u00a0mm, 2.6\u00a0\u03bcm). The mobile phase contained 0.1% formic acid in methanol and water (70:30, v\/v). The internal standard, deuterated 25(OH)D3 and analytes were extracted with hexane. Detection was performed by a mass spectrometer equipped with a triple quadrupole after prior electrospray ionization. It demonstrated sufficient precision and spike recovery within and between days, with a coefficient of variation \u226415% and an error of determination \u226418%. The method exhibited linearity in the 2\u2013100-ng\/mL concentration range. The limits of quantification and limits of detection were 2 and 1\u00a0ng\/mL, respectively. Extraction recoveries ranged from 70.05% to 97.13%. The matrix effect, carryover and dilution integrity were evaluated and met the FDA acceptance criteria. The stability of all metabolites in plasma was confirmed after 3\u00a0h of storage at room temperature and after three cycles of freezing at \u221280\u00b0C and thawing. Applying the method to clinical samples showed a high 25-hydroxyl epimer derived from vitamin D.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf031","type":"journal-article","created":{"date-parts":[[2025,5,30]],"date-time":"2025-05-30T10:24:53Z","timestamp":1748600693000},"source":"Crossref","is-referenced-by-count":1,"title":["Extension of an Ultra-High Performance Liquid Chromatography\u2013MS\/MS Method for the Determination of C3 Epimers of 25-Hydroxyl Derivatives of Vitamin D in Human Plasma"],"prefix":"10.1093","volume":"63","author":[{"given":"Mohamed","family":"Abouzid","sequence":"first","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]},{"name":"Doctoral School, Poznan University of Medical Sciences , Bukowska 70, 60-812 Poznan ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Julia","family":"Kerner","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Aniceta","family":"Mikulska-Sauermann","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]},{"name":"Doctoral School, Poznan University of Medical Sciences , Bukowska 70, 60-812 Poznan ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Dorota","family":"Filipowicz","sequence":"additional","affiliation":[{"name":"Department of Endocrinology, Metabolism and Internal Medicine, Poznan University of Medical Sciences , 49 Przybyszewskiego Street, Poznan 60-355 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Matylda","family":"Resztak","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Franciszek","family":"G\u0142\u00f3wka","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Leonid","family":"Kagan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics and Center of Excellence for Pharmaceutical Translational Research and Education, Ernest Mario School of Pharmacy, Rutgers, The State University of New Jersey , Piscataway, NJ 0885 ,","place":["USA"]}],"role":[{"vocabulary":"crossref","role":"author"}]},{"given":"Marta","family":"Kara\u017aniewicz-\u0141ada","sequence":"additional","affiliation":[{"name":"Department of Physical Pharmacy and Pharmacokinetics, Poznan University of Medical Sciences , 3 Rokietnicka Street, Pozna\u0144 60-806 ,","place":["Poland"]}],"role":[{"vocabulary":"crossref","role":"author"}]}],"member":"286","published-online":{"date-parts":[[2025,5,30]]},"reference":[{"key":"2025053006244525200_ref1","volume-title":"Vitamin and mineral requirements in human nutrition","author":"Organization WH, FAO","year":"2004"},{"key":"2025053006244525200_ref2","doi-asserted-by":"publisher","first-page":"122350","DOI":"10.1016\/j.jchromb.2020.122350","article-title":"Measurement of plasma 25-hydroxyvitamin D2, 25-hydroxyvitamin D3 and 3-epi-25-hydroxyvitamin D3 in population of patients with cardiovascular disease by UPLC-MS\/MS 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