{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T20:27:11Z","timestamp":1762720031285,"version":"build-2065373602"},"reference-count":19,"publisher":"Oxford University Press (OUP)","issue":"9","license":[{"start":{"date-parts":[[2025,10,1]],"date-time":"2025-10-01T00:00:00Z","timestamp":1759276800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2025,10,7]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Sensitive and specific eco-friendly chromatographic approaches were developed to quantitatively determine Dacarbazine along with its United States Pharmacopeia (USP) related impurities simultaneously. Method I was reveresed phase HPLC (RP-HPLC) by which separation of the ternary mixture was achieved. Zorbax Eclipse Plus octadecyl column as stationary phase, a mobile phase formed of double distilled water and methanol at a ratio of (70: 30, v\/v) and 0.6\u00a0mL\/min as a flow rate were used for isocratic separation. Ultra violet (UV) detection was adjusted at 230\u00a0nm. Method II is high performance thin layer chromatography (HPTLC), where the separation was performed on silica gel plates using green developing system composed of butanol: ethyl acetate: 0.05\u00a0M ammonium acetate (2: 4: 0.4, by volume) with UV detection at 280\u00a0nm. Green behavior of the developed techniques was evaluated. The International Council for Harmonisation (ICH) guidelines were considered for the proposed approaches validation. Moreover, Dacarbazine was successfully determined in Dacarbazine medac\u00ae powder for injection by the proposed techniques. No significant difference aroused between the results of the developed techniques and the results aroused from the official USP spectrophotometric method. Furthermore, the proposed methods had the merits of being more friendly towards the environment, sensitive and time saver than other reported HPLC methods.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf053","type":"journal-article","created":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T20:22:12Z","timestamp":1762719732000},"source":"Crossref","is-referenced-by-count":0,"title":["Validated Environmentally Friendly Chromatographic Determinations of Dacarbazine and its Related Substances"],"prefix":"10.1093","volume":"63","author":[{"given":"Ibrahim A","family":"Naguib","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Chemistry, College of Pharmacy, Taif University , P.O.Box 11099, Taif 21944 ,","place":["Saudi Arabia"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Eglal A","family":"Abdelaleem","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University , Alshaheed Shehata Ahmad Hegazy St., 62514 Beni-Suef ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-1011-2837","authenticated-orcid":false,"given":"Eman S","family":"Hassan","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University , Alshaheed Shehata Ahmad Hegazy St., 62514 Beni-Suef ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-0785-4960","authenticated-orcid":false,"given":"Aml A","family":"Emam","sequence":"additional","affiliation":[{"name":"Pharmaceutical Analytical Chemistry Department, Faculty of Pharmacy, Beni-Suef University , Alshaheed Shehata Ahmad Hegazy St., 62514 Beni-Suef ,","place":["Egypt"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2025,11,9]]},"reference":[{"key":"2025110915220673900_ref1","volume-title":"The complete drug reference \u201cthe extra pharmacopoeia\u201d","author":"Martindale","year":"2020","edition":"40th"},{"key":"2025110915220673900_ref2","volume-title":"The United States Pharmacopeia, National Formulary","year":"2021"},{"key":"2025110915220673900_ref3","volume-title":"Safety data sheet of 2- Azahypoxanthine impurity standard","author":"The British Pharmacopoeia, MHRA","year":"2022"},{"key":"2025110915220673900_ref4","doi-asserted-by":"publisher","first-page":"675","DOI":"10.1016\/0731-7085(92)80096-6","article-title":"Degradation of dacarbazine in aqueous solution","volume":"10","author":"Shetty","year":"1992","journal-title":"Journal of Pharmaceutical and Biomedical 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A comprehensive framework for the environmental assessment of solvents","volume":"9","author":"Capello","year":"2007","journal-title":"Green Chemistry"},{"key":"2025110915220673900_ref13","volume-title":"Validation of analytical procedures","author":"ICH, Q2 (R1)","year":"2005"},{"key":"2025110915220673900_ref14","doi-asserted-by":"publisher","first-page":"3813","DOI":"10.1039\/C4OB00705K","article-title":"Practical synthesis of natural plant-growth regulator 2-azahypoxanthine, its derivatives, and biotin-labeled probes","volume":"12","author":"Ikeuchi","year":"2014","journal-title":"Organic & Biomolecular Chemistry"},{"issue":"3","key":"2025110915220673900_ref15","doi-asserted-by":"publisher","first-page":"387","DOI":"10.1093\/jaoacint\/qsae013","article-title":"A robust HPLC approach for quantitation of Camptothecin in mesoporous silica nanoparticles matrix and in the presence of its degradation products","volume":"107","author":"Dave","year":"2024","journal-title":"Journal of AOAC International"},{"key":"2025110915220673900_ref16","doi-asserted-by":"publisher","first-page":"316","DOI":"10.1556\/1006.2015.28.4.8","article-title":"Development and validation of a stability-indicating high-performance thin layer chromatographic method for determination of pyridostigmine bromide in the presence of its alkaline-induced degradation product","volume":"28","author":"Ali","year":"2015","journal-title":"Journal of Planar Chromatography"},{"key":"2025110915220673900_ref17","year":"2004"},{"key":"2025110915220673900_ref18","doi-asserted-by":"publisher","first-page":"757","DOI":"10.1002\/jssc.201401151","article-title":"Validated green high-performance liquid chromatographic methods for the determination of coformulated pharmaceuticals: A comparison with reported conventional methods","volume":"38","author":"Elzanfaly","year":"2015","journal-title":"Journal of Separation Science"},{"key":"2025110915220673900_ref19","doi-asserted-by":"publisher","first-page":"61","DOI":"10.1016\/j.trac.2012.03.013","article-title":"Analytical eco-scale for assessing the greenness of analytical procedures","volume":"37","author":"Ga\u0142uszka","year":"2012","journal-title":"TrAC Trends in Analytical Chemistry"}],"container-title":["Journal of Chromatographic Science"],"original-title":[],"language":"en","link":[{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/63\/9\/bmaf053\/65248287\/bmaf053.pdf","content-type":"application\/pdf","content-version":"vor","intended-application":"syndication"},{"URL":"https:\/\/academic.oup.com\/chromsci\/article-pdf\/63\/9\/bmaf053\/65248287\/bmaf053.pdf","content-type":"unspecified","content-version":"vor","intended-application":"similarity-checking"}],"deposited":{"date-parts":[[2025,11,9]],"date-time":"2025-11-09T20:22:13Z","timestamp":1762719733000},"score":1,"resource":{"primary":{"URL":"https:\/\/academic.oup.com\/chromsci\/article\/doi\/10.1093\/chromsci\/bmaf053\/8317410"}},"subtitle":[],"short-title":[],"issued":{"date-parts":[[2025,10]]},"references-count":19,"journal-issue":{"issue":"9","published-print":{"date-parts":[[2025,10,7]]}},"URL":"https:\/\/doi.org\/10.1093\/chromsci\/bmaf053","relation":{},"ISSN":["0021-9665","1945-239X"],"issn-type":[{"value":"0021-9665","type":"print"},{"value":"1945-239X","type":"electronic"}],"subject":[],"published-other":{"date-parts":[[2025,10]]},"published":{"date-parts":[[2025,10]]},"article-number":"bmaf053"}}