{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,1,26]],"date-time":"2026-01-26T07:09:42Z","timestamp":1769411382858,"version":"3.49.0"},"reference-count":27,"publisher":"Oxford University Press (OUP)","issue":"2","license":[{"start":{"date-parts":[[2026,1,23]],"date-time":"2026-01-23T00:00:00Z","timestamp":1769126400000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,1,23]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>A validated high-performance thin-layer chromatography (HPTLC) method was developed for the simultaneous quantification of bergenin and oleanolic acid in Pashanbhed (Bergenia ligulata), Lambadi (Celosia argentea) and Kalipath (Cissampelos pareira) for quality assessment. The developed HPTLC method employed a mobile phase of toluene, ethyl acetate, methanol and formic acid (4:5:1:2\u00a0v\/v) on silica gel 60F254 plates. The plates were scanned at 254 and 366\u00a0nm using a Camag Scanner IV with vision CATS software. The method was validated for linearity, accuracy, precision, specificity, limit of detection and limit of quantification. The linearity range for bergenin and oleanolic acid was 4\u201320 and 10\u201350\u00a0\u03bcg\/spot, respectively, with correlation coefficients of 0.9998. The average recoveries for bergenin and oleanolic acid were 98.75\u201399.94% and 98.62\u2013100.83%, respectively. The bergenin content in the methanolic extract and crude drug of B. ligulata was 4.8% and 1.25% w\/w, respectively. The oleanolic acid content in the methanolic extract of C. argentea and C. pareira was 1.98% and 1.55%, while in the crude drug, it was 0.24% and 0.16% w\/w, respectively. The developed HPTLC method is simple, accurate and reproducible, making it suitable for routine analysis, standardization and quality control of bergenin and oleanolic acid in various natural herbs and Ayurvedic formulations.<\/jats:p>","DOI":"10.1093\/chromsci\/bmaf065","type":"journal-article","created":{"date-parts":[[2026,1,14]],"date-time":"2026-01-14T12:42:47Z","timestamp":1768394567000},"source":"Crossref","is-referenced-by-count":0,"title":["Development and Validation of a High-Performance Thin-Layer Chromatography Method for Simultaneous Quantification of Bergenin and Oleanolic Acid in Pashanbhed, Lambadi and Kalipath"],"prefix":"10.1093","volume":"64","author":[{"ORCID":"https:\/\/orcid.org\/0000-0003-2997-7053","authenticated-orcid":false,"given":"Sneha S","family":"Oza","sequence":"first","affiliation":[{"name":"School of Pharmacy, RK University , Rajkot, Gujarat 360005 ,","place":["India"]},{"name":"Department of Pharmacognosy, Saraswati Institute of Pharmaceutical Sciences , Dhanap, Gandhinagar, Gujarat 382355 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Vaibhavi","family":"Savalia","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, School of Pharmacy, RK University , Rajkot, Gujarat 360005 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Umang","family":"Gajjar","sequence":"additional","affiliation":[{"name":"Department of Pharmacognosy, Saraswati Institute of Pharmaceutical Sciences , Dhanap, Gandhinagar, Gujarat 382355 ,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,1,23]]},"reference":[{"issue":"1","key":"2026012307321836700_ref1","doi-asserted-by":"publisher","first-page":"25","DOI":"10.1016\/j.jep.2005.09.024","article-title":"Integrated approaches towards drug development from Ayurveda and other Indian system of medicines","volume":"103","author":"Mukherjee","year":"2006","journal-title":"Journal of Ethnopharmacology"},{"key":"2026012307321836700_ref2","doi-asserted-by":"publisher","first-page":"352","DOI":"10.1556\/1326.2024.01265","article-title":"Quantify biomarkers in Asthimajja Pachak Kwath by high performance thin layer chromatography","volume":"37","author":"Patel","year":"2024","journal-title":"Acta Chromatographica"},{"issue":"4","key":"2026012307321836700_ref3","first-page":"267","article-title":"Recurrence after a single renal stone in a community practice","volume":"11","author":"Sutherland","year":"1985","journal-title":"Mineral and Electrolyte Metabolism"},{"issue":"2","key":"2026012307321836700_ref4","first-page":"97","article-title":"Pashanbheda\u2014a golden herb of Himalaya: A review","volume":"2","author":"Ruby","year":"2012","journal-title":"International Journal of Pharmacy Review & Research"},{"key":"2026012307321836700_ref5","doi-asserted-by":"publisher","first-page":"85","DOI":"10.1016\/j.jep.2014.10.013","article-title":"The most potent antilithiatic agent ameliorating renal dysfunction and oxidative stress from Bergenia ligulata rhizome","volume":"158","author":"Aggarwal","year":"2014","journal-title":"Journal of Ethnopharmacology"},{"issue":"4","key":"2026012307321836700_ref6","doi-asserted-by":"publisher","first-page":"351","DOI":"10.1002\/ptr.833","article-title":"In vitro studies on antilithiatic activity of seeds of Dolichos biflorus Linn. and rhizomes of Bergenia ligulata wall","volume":"15","author":"Garimella","year":"2001","journal-title":"Phytotherapy Research"},{"issue":"33","key":"2026012307321836700_ref7","doi-asserted-by":"publisher","first-page":"7","DOI":"10.5530\/pj.2012.33.2","article-title":"Pharmacognostic and phytochemical evaluation of Celosia argentea","volume":"4","author":"Ghorpade","year":"2012","journal-title":"Pharmacognosy Journal"},{"issue":"4","key":"2026012307321836700_ref8","doi-asserted-by":"publisher","first-page":"526","DOI":"10.1248\/bpb.25.526","article-title":"Anti-diabetic activity of alcoholic extract of Celosia argentea Linn. 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