{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T17:19:14Z","timestamp":1771003154057,"version":"3.50.1"},"reference-count":16,"publisher":"Oxford University Press (OUP)","issue":"3","license":[{"start":{"date-parts":[[2026,2,13]],"date-time":"2026-02-13T00:00:00Z","timestamp":1770940800000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"funder":[{"DOI":"10.13039\/100006545","name":"NIMHD","doi-asserted-by":"publisher","award":["U54MD007590 (Project 1) (Wu)"],"award-info":[{"award-number":["U54MD007590 (Project 1) (Wu)"]}],"id":[{"id":"10.13039\/100006545","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NCI","doi-asserted-by":"publisher","award":["R01CA256058"],"award-info":[{"award-number":["R01CA256058"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100000054","name":"NCI","doi-asserted-by":"publisher","award":["R42CA217491"],"award-info":[{"award-number":["R42CA217491"]}],"id":[{"id":"10.13039\/100000054","id-type":"DOI","asserted-by":"publisher"}]},{"DOI":"10.13039\/100006545","name":"NIMHD","doi-asserted-by":"publisher","award":["U54MD007605"],"award-info":[{"award-number":["U54MD007605"]}],"id":[{"id":"10.13039\/100006545","id-type":"DOI","asserted-by":"publisher"}]}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,2,13]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>SLP-1A6 is a nicardipine-derived analog that modulates the embryonic ectoderm development\/Enhancer of Zeste Homolog 2 axis and holds promise as novel therapeutics for castration-resistant prostate cancer (PCa). We report here the development and validation of a robust, sensitive and specific LC\u2013MS\/MS method for quantifying SLP-1A6 in rat plasma, as part of its preclinical investigation. The method demonstrated excellent linearity, precision, accuracy, extraction recovery and stability with negligent matrix effect over a 5\u20132500\u00a0ng\/mL concentration range. The method was successfully applied for a single intravenous dose pharmacokinetic (PK) study in adult male Sprague\u2013Dawley rats and the resulting PK parameters were comparable to those of nicardipine, indicating that SLP-1A6 retains the favorable PK profile of the parent compound while offering in vivo efficacy for chemoresistant PCa. These findings support the further preclinical development of SLP-1A6 to overcome chemoresistance in PCa.<\/jats:p>","DOI":"10.1093\/chromsci\/bmag004","type":"journal-article","created":{"date-parts":[[2026,1,27]],"date-time":"2026-01-27T12:11:57Z","timestamp":1769515917000},"source":"Crossref","is-referenced-by-count":0,"title":["Method Development and Validation of Liquid Chromatography Tandem Mass Spectrometry for SLP-1A6 in Rat Plasma"],"prefix":"10.1093","volume":"64","author":[{"given":"Yuan","family":"Chen","sequence":"first","affiliation":[{"name":"College of Pharmacy and Health Sciences, Texas Southern University , 3100 Cleburne Street, Houston, TX 77004 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Tolulope","family":"Adebusuyi","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Health Sciences, Texas Southern University , 3100 Cleburne Street, Houston, TX 77004 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Texas Southern University , 3100 Cleburne Street, Houston, TX 77004 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Dong","family":"Liang","sequence":"additional","affiliation":[{"name":"College of Pharmacy and Health Sciences, Texas Southern University , 3100 Cleburne Street, Houston, TX 77004 ,","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"San L","family":"Pham","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Emory University , 1515 Dickey Dr., Atlanta, GA 30322 \u00a0","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Frank E","family":"McDonald","sequence":"additional","affiliation":[{"name":"Department of Chemistry, Emory University , 1515 Dickey Dr., Atlanta, GA 30322 \u00a0","place":["USA"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Alira","family":"Danaher","sequence":"additional","affiliation":[{"name":"Center for Cancer Research and 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