{"status":"ok","message-type":"work","message-version":"1.0.0","message":{"indexed":{"date-parts":[[2026,5,5]],"date-time":"2026-05-05T02:46:08Z","timestamp":1777949168136,"version":"3.51.4"},"reference-count":29,"publisher":"Oxford University Press (OUP)","issue":"5","license":[{"start":{"date-parts":[[2026,4,30]],"date-time":"2026-04-30T00:00:00Z","timestamp":1777507200000},"content-version":"vor","delay-in-days":0,"URL":"https:\/\/academic.oup.com\/pages\/standard-publication-reuse-rights"}],"content-domain":{"domain":[],"crossmark-restriction":false},"short-container-title":[],"published-print":{"date-parts":[[2026,4,30]]},"abstract":"<jats:title>Abstract<\/jats:title>\n                  <jats:p>Clomiphene citrate (CLO) and co-enzyme Q10 (Q10) are \u201covulatory stimulants\u201d used to treat female infertility and CLO-resistant Polycystic Ovary Syndrome (PCOS). The study aimed to create a unique and well-validated stability-indicating RP-HPLC method for quantifying CLO and Q10 in bulk and tablet formulation, supported by UV analysis. The \u03bb max values of CLO and Q10 were determined at 293\u00a0nm and 273\u00a0nm, respectively. The chromatographic separation was achieved on a Waters XBridge phenyl 5\u00a0\u03bcm column (4.6\u2009\u00d7\u2009250\u00a0mm) using an acetonitrile: tetrahydrofuran (80:20\u00a0v\/v) with 0.5% triethylamine as a mobile phase at a flow rate of 0.5\u00a0mL\/min with photodiode array detector at 279\u00a0nm (isobestic point). Resveratrol (RES) served as an internal standard (ISTD), with retention durations of 6.504, 9.319 and 5.831\u00a0minutes for CLO, Q10 and RES, respectively. Calibration curves were linear (R2\u2009=\u20090.9987 for CLO and 0.9993 for Q10) and %RSD values for all validation parameters were below 2%, confirming precision and accurate. Forced degradation studies through various stress conditions, assessed the method\u2019s stability-indicating nature by exhibiting clear separation of degradation products from the analytes and degradation levels within ICH limits (\u2264 20%). The validated method was found to be reliable, specific, and applicable for routine analysis in quality control.<\/jats:p>","DOI":"10.1093\/chromsci\/bmag013","type":"journal-article","created":{"date-parts":[[2026,4,9]],"date-time":"2026-04-09T11:45:00Z","timestamp":1775735100000},"source":"Crossref","is-referenced-by-count":0,"title":["Development and validation of stability-indicating RP-HPLC method for simultaneous estimation of clomiphene citrate and co-enzyme Q10 in bulk and pharmaceutical dose"],"prefix":"10.1093","volume":"64","author":[{"given":"Malavika","family":"Thiruvenkatakumar","sequence":"first","affiliation":[{"name":"Department of Pharmaceutical Analysis, PSG College of Pharmacy , Coimbatore, Tamil Nadu 641004,","place":["India"]},{"name":"Manipal Institute of Technology, Manipal Academy of Higher Education , Manipal, Karnataka 576104,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"ORCID":"https:\/\/orcid.org\/0000-0002-4198-6318","authenticated-orcid":false,"given":"Hariprasad","family":"Ranganathan","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutical Analysis, PSG College of Pharmacy , Coimbatore, Tamil Nadu 641004,","place":["India"]},{"name":"Department of Pharmaceutical analysis, Faculty of Pharmacy, Karpagam Academy of Higher Education , Coimbatore, Tamil Nadu 641021,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]},{"given":"Habibur Rahman","family":"S M","sequence":"additional","affiliation":[{"name":"Department of Pharmaceutics, PSG College of Pharmacy , Coimbatore, Tamil Nadu 641004,","place":["India"]}],"role":[{"role":"author","vocabulary":"crossref"}]}],"member":"286","published-online":{"date-parts":[[2026,5,3]]},"reference":[{"key":"2026050307483739100_ref1","first-page":"68","article-title":"Instrumental method of 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